miR126-5p Downregulation Facilitates Axon Degeneration and NMJ Disruption via a Non-Cell-Autonomous Mechanism in ALS.

Axon degeneration and disruption of neuromuscular junctions (NMJs) are key events in amyotrophic lateral sclerosis (ALS) pathology. Although the disease\u27s etiology is not fully understood, it is thought to involve a non-cell-autonomous mechanism and alterations in RNA metabolism. Here, we identified reduced levels of miR126-5p in presymptomatic ALS male mice models, and an increase in its targets: axon destabilizing Type 3 Semaphorins and their coreceptor Neuropilins. Using compartmentalize

Suggested model.

<p>In order to arrive at the cell body and subsequently the CNS, rabies virus hijacks a fast route using the p75NTR endosomal pathway. In a p75NTR dependent path, RABV manipulates the axonal transport machinery to migrate faster to the cell body. An alternative, slower path, may involve alternative RABV receptors.</p

Rabies virus retrograde transport in DRG is faster and more directed than that of NGF.

<p>Retrograde transport of (<b>A</b>) EGFP-RABV and (<b>B</b>) Quantum-dot conjugated NGF in DRG explants, roughly 2 hours after addition to distal axon compartment. Arrows and arrowheads pointing at transported particles. Scale bars = 10 µm. (<b>C,D</b>) Kymograph for EGFP-RABV and NGF respectively. Horizontal scale bars = 5 µm, vertical scale bars = 40 seconds. (<b>E–J</b>) Characterization of manually tracked directed particles of RABV (n = 209) and NGF (n = 149). (<b>E</b>) RABV is transported faster than NGF, as seen by average speed. (<b>F</b>) RABV is more directed and pauses less than NGF (<b>G</b>) Average pause duration was not significantly different. (<b>H</b>) RABV spent a smaller fraction of its run paused (<b>I</b>) Instantaneous velocities of RABV particles (n = 9885 events) are higher than those of NGF particles (n = 8973 events). Positive and negative values represent retrograde and anterograde velocities, respectively. (<b>J</b>) RABV particles travel larger net distances than NGF's, as seen by mean squared displacements. Data was pulled from two separate experiments. Error bars represent SEM, *p<0.0001.</p

RABV travels faster and is more directed when transported with p75NTR.

<p>(<b>A–C</b>) Multi-channel live imaging of EGFP-RABV 2 hours after addition to distal axon compartment of DRG explant previously treated with a fluorescent antibody against p75NTR. Arrowheads: p75NTR-positive RABV puncta, scale bar = 10 µm. (<b>D,E</b>) Kymographs of and P75NTR extracted from time lapse depicted in (A–C). (<b>F</b>) RABV-only tracks (green) are less directed than RABV-p75NTR tracks (yellow), as shown when overlaying corresponding kymographs. Vertical scale bar = 5 µm, horizontal scale bar = 40 seconds. (<b>G–O</b>) Characterization of directed RABV puncta, transported with and without p75NTR, n = 184 and n = 122, respectively. (G) RABV presents higher speeds when transported with p75NTR, due to less frequent (H) and shorter pauses (I). Overall RABV-p75NTR spent less time paused on average (J), Diameter and intensity measurements revealed that RABV puncta positive for p75NTR were larger (<b>K</b>) and had higher intensity levels (<b>L</b>) than p75NTR-negative puncta. (<b>M–O</b>) p75NTR positive puncta (blue) are faster, more directed and present higher displacements over time, compared to p75NTR negative puncta (red), illustrated by distribution of instantaneous velocities in (M) (RABV+p75: n = 8051 events; RABV-p75: n = 7423 events) displacement plotted over time (N) and mean square displacement (O). Data is pulled from two separate experiments, error bars represent SEM. *p<0.05.</p

RABV binds and internalizes with p75NTR in DRG neuron tips.

<p>Co-localization of EGFP-RABV with p75NTR is shown by live TIRF imaging and sub-pixel localization algorithms. (<b>A</b>) RABV-p75 particles shift from the periphery to the center of the growth cone, where they are internalized into the cell. Lower panels zoom in on dashed square, showing co-localized puncta (left) shifting towards the center of the growth cone (middle) until finally internalized (right). (<b>B</b>) Presentation of six separate events of RABV and p75NTR binding and internalization on the surface of the growth cone shown in (A). Colored trajectories denote displacement from point of detection to point of disappearance. (<b>C</b>) RABV and p75NTR are internalized together, illustrated by corresponding plots of puncta intensity over time (normalized to background), calculated for co-localized particles shown in lower panels of (A). Scale bars = 5 µm. (<b>D</b>) Zoom-in on colocalized RABV and p75 spot, taken from panel (A), scale bar = 1 µm. (<b>E</b>) Overlay of 1D-Gaussian fits of p75 and RABV intensity profiles at the x-axis of the image in panel (D). (<b>F</b>) Representative overlay of radial symmetry fits of the x-y intensity profiles of p75 and RABV spots. σ is the standard deviation of each fitting function; distance between the two spot centers is 51.3 nm. (<b>G</b>) Knockdown of p75NTR decreases rabies virus infection for shorts time incubation. DRGs embryonic cells infected with lentiviral vectors (LV) containing 4 different EGFP-tagged shRNA's against p75NTR or LV-EGFP, were transfected with RABV for 30 or 120 minutes. Low levels of infected neurons were found in shRNA-p75-EGFP cells Average RABV infection rates were normalized to LV-EGFP controls (n = 4 experiments, error bars = SEM, *p<0.005, **p<0.0005).</p

Co-transport of RABV and NGF.

<p>(<b>A–C</b>) EGFP-RABV and Qdot-NGF were simultaneously added to the distal compartment of a DRG explant at 3DIV. Dual-channel live imaging revealed multiple events of RABV co-transported with NGF, illustrated by corresponding images from either channel and overlay. Arrowheads: co-localized puncta trafficked over time. Scale bar = 10 µm. (<b>D–F</b>) Kymographs of EGFP-RABV and Qdot-NGF show mutual transport of RABV and NGF. (<b>G</b>) Two populations of RABV-NGF particles were identified by distribution of average track speeds, as seen by fitted curve (red, minima point represented by dashed line, n = 46). (<b>H–J</b>) “Slow” (n = 38) and “fast” (n = 8) populations according to the minima (1.01 µm/sec) in (G). “Slow” tracks paused more than “fast” tracks (<b>H</b>), and though stop duration was not significantly different (<b>I</b>), spent a larger fraction of their travel paused (<b>J</b>). Data is pulled from two experiments. Error bars represent SEM, *p<0.02.</p

A microfluidic system for tracking retrograde transport in sensory axons.

<p>(<b>A</b>) A Polydimethylsiloxane (PDMS) microfluidic chamber used for explant culture. (<b>B,C</b>) 40 µl interval towards the proximal compartment (top) prevents fluorescent dye from diffusing to the proximal compartment, allowing several hours of compartmental separation. Bright field (<b>B</b>) and fluorescent images (<b>C</b>) taken 7.5 hours after addition of Sulforhodamine B fluorescent dye to the distal compartment (bottom). (<b>D</b>) DRG explants are healthy and extend axons through micro-grooves to distal compartment after 2–3 days in vitro. One microgroove typically contains 2–5 axons. Mosaic of 10× images of Calcein-stained DRG explant taken after 5 DIV. Scale bar = 50 µm.</p