Currently used in clinical practice beam rate changes have no significant effect on the reduction of clonogenic capacity of PNT1A cells in vitro

Background: Due to the lack of selectivity of ionizing radiation between normal and cancer cells, it is important to improve the existing radiation patterns. Lowering the risk of cancer recurrence and comfort during treatment are priorities in radiotherapy. Materials and methods: In the experiment we used dose verification to determine the irradiation time calculated by a treatment planning system for 6XFFF and 10XFFF beams. Cells cultured under standard conditions were irradiated with a dose of 2 Gy at different beam rates 400 MU/min, 600 MU/min, 800 MU/min, 1000 MU/min, 1400 MU/min,  1600 MU/min and 2400 MU/min using 6XFFF, 10XFFF and 6XFF beams. Results: The experiment was aimed at comparing the biological response of normal prostate cells after clinically applied radiation patterns. No statistically significant differences in the cellular response were observed. The wide range of beam rates as well as the beam profiles did not significantly affect cell proliferation. Conclusions: High beam rates, without significantly affecting the clonogenic capacity of cells, have an impact on the quality of patient's treatment. With the increasing beam rate the irradiation time is shortened, which has an important impact on patients’ health. This experiment can have a practical significance

Pro Libris: Lubuskie Pismo Literacko-Kulturalne, nr 4 (2010)

145 s. : il., portr.

Pro Libris: Lubuskie Pismo Literacko-Kulturalne, nr 2 (2009)

123 s. : il.

Comparison of three different phantoms used for Winston-Lutz test with Artiscan software

BackgroundOne of the most important test in every quality assurances process of medical linear accelerators is the Winston-Lutz test, allowing an evaluation of the treatment isocentre in the light of uncertainty of the position of the collimator, the gantry and the couch.AimThe purpose of this work was analysis of the results of the Winston-Lutz test performed with three different phantoms for two different accelerators.Materials and methodsMeasurements were performed on two Varian machines: TrueBeam equipped with aS1200 EPID and TrueBeam equipped with aS1000 EPID. During the study three different phantoms dedicated for verification of the radiation isocentre were used: PTW Isoball, AQUILAB Isocentre Phantom and Varian Isocentre Cube. Analysis of the DICOM images was performed in Artiscan software.ResultsFor TrueBeam with as1200 EPID, gantry MV isocentre was about 0.18 mm larger for Varian Isocentre Cube than for two other phantoms used in this study. The largest variability of this parameter was observed for the couch. The results differed to 1.16 mm. For TrueBeam with as1000 EPID, results for collimator isocentre with PTW Isoball phantom were about 0.10 mm larger than for two other phantoms. For the gantry, results obtained with Varian Isocentre Cube were 0.21 mm larger.ConclusionThe obtained results for all three phantoms are within the accepted tolerance range. The largest differences were observed for treatment couch, which may be related to the phantom mobility during couch movement

An Analysis of Variability in the Content of Phenolic Acids and Flavonoids in Camelina Seeds Depending on Weather Conditions, Functional Form, and Genotypes

Camelina oil obtained from the seeds of Camelina sativa exhibits strong antioxidative properties. This study was based on four years of field experiments conducted on 63 genotypes of spring and 11 genotypes of winter camelina. The aim of the study was to determine the variability in the content of the selected bioactive compounds, depending on the weather conditions during the cultivation, the functional form, and genotype. The cultivation form of the genotypes analysed in our study did not exhibit significant differences in the quantitative profiles of the tested phenolic acids and flavonoids. Sinapic acid was the most abundant of all phenolic acids under analysis (617–668 mg/kg), while quercetin was the main flavonoid (91–161 mg/kg). Camelina has great potential not only for the food industry but also for researchers attempting to breed an oil plant with the stable biosynthesis of bioactive compounds to ensure oxidative protection of obtained fat

The Effect of Mineral Fertilization on the Content of Bioactive Compounds in Hemp Seeds and Oil

The popularity of hemp cultivation for industrial purposes has been steadily growing for many years. With the addition of products derived from these plants to the Novel Food Catalogue, maintained by the European Commission, a significant increase in interest in hemp food is also expected. The aim of the study was to determine the characteristics of hempseed, oil, and oil cake samples produced from experimental plots grown in different conditions. The research was conducted on the Henola variety, one of the newest and most popular varieties of hemp, recently bred for grain and oil. The content of bioactive compounds in grain and oil has been subjected to detailed chemical analyses in order to determine the effect of fertilization, the method of plant cultivation, and processing conditions on their quantity. The test results and the statistical analysis carried out showed a significant impact of the tested factors on the content of some of the tested bioactive compounds. The obtained results will help in the development of an effective method of cultivation for this hemp variety in order to maximize the content of the desired bioactive compounds per unit of cultivation area

Food-dependent exercise-induced anaphylaxis (FDEIA) in a nectarine-sensitized patient – case study

Diagnostyka reakcji anafilaktycznych po spożyciu pokarmów przysparza trudności, szczególnie w przypadku współwystępowania kofaktorów reakcji alergicznych. Wprowadzenie możliwości oznaczenia IgE swoistych dla komponent alergenowych zwiększyło możliwości diagnostyczne. Prezentujemy ciekawy przypadek pacjentki diagnozowanej z powodu kilkukrotnie przebytych reakcji anafilaktycznych w trakcie podejmowanego wysiłku fizycznego po spożyciu pokarmów takich jak nektarynka, jabłko, śliwka, winogrono, orzech laskowy, orzech włoski, piwo pszeniczne jagodowe. W badaniu ImmunoCAP ISAC stwierdzono podwyższony poziom IgE dla białek transportujących lipidy orzecha włoskiego, brzoskwini, bylicy, platana, pszenicy (Jug r 3, Pru p 3, Art v 3, Pla a 3, Tri a 14). U pacjentki rozpoznano anafilaksję zależną od pokarmu indukowaną wysiłkiem fizycznym oraz alergię na białka transportujące lipidy.Diagnostics of allergic reactions after food consumption cause trouble, especially when allergic reactions cofactors coexist. Introducing the possibility of marking IgE specific to allergen components increase diagnostics possibilities. We present interesting case of patient who was diagnosed due to an interview of several anaphylactic reaction during physical effort after consumption of food like nectarine, apple, plum, grapes, hazelnut, walnut, wheat berry beer. During diagnosis, in the ImmunoCAP ISAC test, an increased level of IgE was found for lipid transfer proteins of walnut, peach, mugwort, plane tree, wheat (Jug r 3, Pru p 3, Art v 3, Pla a 3, Tri a 14). The patient was diagnosed with food-dependent exercise-induced anaphylaxis associated with an allergy to lipid transfer proteins (LTPs)