9 research outputs found
The Read-Across Hypothesis and Environmental Risk Assessment of Pharmaceuticals
Pharmaceuticals in
the environment have received increased attention
over the past decade, as they are ubiquitous in rivers and waterways.
Concentrations are in sub-ng to low μg/L, well below acute toxic
levels, but there are uncertainties regarding the effects of chronic
exposures and there is a need to prioritise which pharmaceuticals
may be of concern. The read-across hypothesis stipulates that a drug
will have an effect in non-target organisms only if the molecular
targets such as receptors and enzymes have been conserved, resulting
in a (specific) pharmacological effect only if plasma concentrations
are similar to human therapeutic concentrations. If this holds true
for different classes of pharmaceuticals, it should be possible to
predict the potential environmental impact from information obtained
during the drug development process. This paper critically reviews
the evidence for read-across, and finds that few studies include plasma
concentrations and mode of action based effects. Thus, despite a large
number of apparently relevant papers and a general acceptance of the
hypothesis, there is an absence of documented evidence. There is a
need for large-scale studies to generate robust data for testing the
read-across hypothesis and developing predictive models, the only
feasible approach to protecting the environment
Effects of fluoxetine on fish - Dataset - Margiotta-Casaluci et al. 2014
The file provides the results of a study during which male fathead minnows were exposed for 28 days to the anti-depressant fluoxetine
Effect of fluoxetine on fish exploratory behaviour quantified during a Novel Tank Diving Test performed after 14 days of exposure.
<p>A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area. C1 and C2 indicate control group 1 and control group 2, respectively. Boxes represent medians (full line), with 5th and 95th percentiles (<i>n</i> = 20). *<i>p</i><0.05.</p
Relationship between plasma concentrations of norfluoxetine and its effects on fish exploratory behaviour after 28 days of exposure.
<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 72–258 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p
Inter-individual and intra-treatment variability of fluoxetine plasma concentrations.
<p>The unit of the values indicated in the columns Mean, Median, Min, Max, 25%, and 75% is ng/mL.</p><p>Inter-individual and intra-treatment variability of fluoxetine plasma concentrations.</p
Methodological procedure for the quantification of fish behaviour.
<p>A) Experimental steps performed to quantify fish behavioural response to a new environment following 14-day and 28-day exposure to fluoxetine. Anxiety-related behavioural endpoints were quantified using a Novel Tank Diving Test. B) Example of different exploratory behaviours in a Novel Tank Diving Test. Inactive fish (left) <i>versus</i> active fish (right). The different tracking colours indicate different speeds (black, slow; green, medium; red, fast).</p
Relationship between plasma concentrations of fluoxetine and behavioural endpoints expressed as median values.
<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test after 28 days of exposure. The dose-response of behavioural endpoints appears to be visually more obvious when median values are used (in this figure) instead of mean values (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110467#pone-0110467-g004" target="_blank">Figure 4</a>). This highlights the important role of inter-individual variability in the interpretation of behavioural effects. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 91–302 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as medians (<i>n</i> = 20). *: <i>p</i><0.05.</p
Relationship between plasma concentrations of fluoxetine plus norfluoxetine and their effects on fish exploratory behaviour after 28 days of exposure.
<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine + norfluoxetine plotted in the graphs is 163–560 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p
Relationship between plasma concentrations of fluoxetine and its effects on fish exploratory behaviour after 28 days of exposure.
<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 91–302 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p