3,956 research outputs found
Surface excitonic emission and quenching effects in ZnO nanowire/nanowall systems: limiting effects on device potential.
We report ZnO nanowire/nanowall growth using a two-step vapour phase transport method on a-plane sapphire. X-ray diffraction and scanning electron microscopy data establish that the nanostructures are vertically well-aligned with c-axis normal to the substrate, and have a very low rocking curve width. Photoluminescence data at low temperatures demonstrate the exceptionally high optical quality of these structures, with intense emission and narrow bound exciton linewidths. We observe a high energy excitonic emission at low temperatures close to the band-edge which we assign to the surface exciton in ZnO at ~ 3.366 eV, the first time this feature has been reported in ZnO nanorod systems. This assignment is consistent with the large surface to volume ratio of the nanowire systems and indicates that this large ratio has a significant effect on the luminescence even at low temperatures. The band-edge intensity decays rapidly with increasing temperature compared to bulk single crystal material, indicating a strong temperature-activated non-radiative mechanism peculiar to the nanostructures. No evidence is seen of the free exciton emission due to exciton delocalisation in the nanostructures with increased temperature, unlike the behaviour in bulk material. The use of such nanostructures in room temperature optoelectronic devices appears to be dependent on the control or elimination of such surface effects
In vitro tissue microarrays for quick and efficient spheroid characterisation
Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques for 3D models are currently limited to simplistic live/dead assays. By sectioning and staining in vitro microtissues researchers can examine their structure, detect DNA, RNA and protein targets and visualise them at the level of single cells. The morphological examination and immunochemistry staining for in vitro cultures has historically been done in a laborious manner involving testing one set of cultures at a time. We have developed a technology to rapidly screen spheroid phenotype and protein expression by arranging 66 spheroids in a gel array for paraffin-embedding, sectioning and immunohistochemsitry. The process is quick, mostly automatable and uses 11 times less reagents compared to conventional techniques. Here we showcase the capabilities of the technique in an array made up of 11 different cell lines stained in conventional H&E staining, as well as immunohistochemistry staining for estrogen (ER), progesterone (PR) human epidermal growth factor receptors (Her-2) and TP53. This new methodology can be used in optimising stem cell-based models of disease and development, for tissue engineering, safety screening and for efficacy screens in cancer research
Modular classes of skew algebroid relations
Skew algebroid is a natural generalization of the concept of Lie algebroid.
In this paper, for a skew algebroid E, its modular class mod(E) is defined in
the classical as well as in the supergeometric formulation. It is proved that
there is a homogeneous nowhere-vanishing 1-density on E* which is invariant
with respect to all Hamiltonian vector fields if and only if E is modular, i.e.
mod(E)=0. Further, relative modular class of a subalgebroid is introduced and
studied together with its application to holonomy, as well as modular class of
a skew algebroid relation. These notions provide, in particular, a unified
approach to the concepts of a modular class of a Lie algebroid morphism and
that of a Poisson map.Comment: 20 page
Kinetics of gas emission from aluminosilicates used as a relaxing additive for moulding and core sands
The article presents the results of gas emissions generated during heating of mineral additives – aluminosilicates (perlite ore and vermiculite). The test on a laboratory stand for a 1 g sample at 1 000 °C was carried out. It has been shown, that there is a correlation between the degree of fragmentation and the amount of gas generated. The finest fraction of perlite ore caused a similar quantitative gas emission as ground vermiculite. The presence of additives in molding sands, regardless of the size of fraction, should not affect the formation of casting defects. The addition of perlite ore and vermiculite does not effect the ecological properties of moulding sand
Time-dependent Mechanics and Lagrangian submanifolds of Dirac manifolds
A description of time-dependent Mechanics in terms of Lagrangian submanifolds
of Dirac manifolds (in particular, presymplectic and Poisson manifolds) is
presented. Two new Tulczyjew triples are discussed. The first one is adapted to
the restricted Hamiltonian formalism and the second one is adapted to the
extended Hamiltonian formalism
Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines
Introduction. Studies on expression of orexins (OXs) and their receptors in human prostate gland and human prostatic cell lines are scanty and their results contradictory. Regarding this, we carefully reinvestigated this problem on human prostatic cell lines.
Material and methods. Expression of preproorexin (ppOX) (6 primer pairs), and orexin receptors 1 and 2 (OXR1, OXR2) (4 and 2 primer pairs, respectively) was assessed by conventional PCR and QPCR in human normal (PrEC, PrSc, PrSmC) and prostate carcinoma (Du145, LNCaP, and PC3) cell lines. We designed intron spanning primers and also we applied primers from earlier publications and commercially available ones.
Results. With the designed primer pairs, in all studied cell lines we failed to demonstrate expression of ppOX, OXR1 and OXR2 genes at the mRNA level, while reaction products were observed in control tissues (human placenta and adrenals). Primers applied in earlier studies did not form amplification products specific for preproorexin or orexin 1 receptor. Some commercially available primers for orexin receptor 1 produced false positive results.
Conclusions. We found no evidence for the presence of preproorexin–orexin receptors system genes’ mRNAs in human prostate cell lines. The reported premises for these genes’ expression in prostate and prostatic cell lines may have arisen either from the presence of non-prostate cells included in the samples or from faulty PCR settings
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