QUALITY AND HYGIENIC CONDITIONS OF WHITE LUPIN SILAGE, AFFECTED BY FORAGE STAGE OF GROWTH AND USE OF SILAGE ADDITIVES

ABSTRACT A two-factor field experiment with white lupin cv. Butan was carried out. The first factor was the green forage harvest date (the flat pod stage -Cut 1 and the stage of green ripe seeds -Cut 2), while the second one -application of silage additives: biological (strains of lactic acid bacteria) and chemical (a mixture of organic acids), and the control treatment (without additives). In Cut 2 higher fresh matter (FM) and dry matter (DM) yields were obtained. Silage inoculated with the biological additive contained a significantly greater count of lactic acid bacteria. Both additives reduced counts the Clostridium bacteria, yeasts and mould fungi. The silage with the chemical additive had a three-fold higher content of water-soluble carbohydrates (WSC), while the biological additive increased lactic acid (LA) levels. White lupin can be used as a silage raw material, but plants before ensiling should be partially wilted and silage additives should be applied

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals in <em>A. thaliana</em> plants grown under iron deficiency

International audienceMounting evidence indicate that nitric oxide (NO) acts as a signaling molecule mediating iron deficiency responses through the upregulation of the expression of iron uptake-related genes. Accordingly, NO donors such as nitrosoglutathione (GSNO) were reported to improve the fitness of plants grown under iron deficiency. Here, we showed that glutathione, a by-product of GSNO, triggered the upregulation of the expression of iron uptake- and transport-related gene and an increase of iron concentration in Arabidopsis thaliana seedlings facing iron deficiency. Furthermore, we provided evidence that under iron deficiency, NO released by GSNO did not improve the root iron concentration but impacted the content of copper. Collectively, our data highlight the complexity of interpreting data based on the use of NO donors when investigating the role of NO in iron homeostas