Determination of the Lipophilicity of Some New Derivatives of Thiosemicarbazide and 1,2,4-triazoline-5-thione with Potential Antituberculosis Activity

The chromatographic behavior of newly obtained derivatives of thiosemicarbazide and 1,2,4- triazoline-5-thione was determined. The lipophilicity was confirmed by the use of the Reversed Phase Thin-Layer Chromatography (RP-TLC) method. For both groups of solutes the lipophilicity depended on the substituents. All obtained compounds were tested for their antimycotic activity. The strongest antituberculosis activity was observed for 4-(2-iodophenyl)-1-(pyridine-4-ylacetyl)thiosemicarbazide 4 and 4- phenyl-3-(pyridine-4-ylmethyl)-1,2,4-triazoline-5-thione 27

Determination of the Lipophilicity of Some New Derivatives of Thiosemicarbazide and 1,2,4-triazoline-5-thione with Potential Antituberculosis Activity

The chromatographic behavior of newly obtained derivatives of thiosemicarbazide and 1,2,4- triazoline-5-thione was determined. The lipophilicity was confirmed by the use of the Reversed Phase Thin-Layer Chromatography (RP-TLC) method. For both groups of solutes the lipophilicity depended on the substituents. All obtained compounds were tested for their antimycotic activity. The strongest antituberculosis activity was observed for 4-(2-iodophenyl)-1-(pyridine-4-ylacetyl)thiosemicarbazide 4 and 4- phenyl-3-(pyridine-4-ylmethyl)-1,2,4-triazoline-5-thione 27

Essential oils from the Herba and fruits of Peucedanum luxurians and their antituberculosis activity

The Apiaceae family has been accompanying people for thousands of years, being present in the kitchen, as well as in the pharmacy. Plants belonging to this family are well known as sources of coumarins and essential oils.                Essential oils from the Herba, as well as fruits, of Peucedanum luxurians Tamamsch. (an endemic umbelliferous plant taxon from Armenia) were obtained by hydrodistillation in a Deryng apparatus for the first time. The GC-MS analyses showed the presence of trans-β-farnesene (16%) and germacrene D (13%) as the most abundant components of the essential oils.                One of the most valuable properties of essential oils is their antimicrobial activity. It is a very desirable feature, especially in the case of some bacteria, which cause huge health problems. A good example is Mycobacterium tuberculosis, one of the leading causes of human morbidity and mortality.                The activity of essential oils from different parts of P. luxurians was tested for antituberculosis activity. Minimal Inhibitory Concentrations (MIC) values for the essential oils were determined by a 96-well microplate method with alamarBlue (Invitrogen). The inoculum of the reference strain of Mycobacterium tuberculosis H37Ra in Middlebrook 7H9 broth (Difco) was 5 x 105 cfu/mL per well, according to CLSI standards. Serial twofold dilutions of essential oils ranged from 8 to 256 µg/mL. As the internal control of the method, serial twofold dilutions of four first-line antibiotics dedicated to tuberculosis treatment: isoniazid (INH), rifampicin (RMP), ethambutol (EMB), and streptomycin (SM) were used [1,2]

Volatile compounds from different species of Lycopodium with anti-tuberculosis activity

Different species belonging to the genus Lycopodium L. (Lycopodiaceae) were used in folk medicine due to their antibacterial, healing effects on wounds, and properties used in the treatment of mental diseases, like amnesia, schizophrenia and different types of dementia.                Extracts containing volatiles obtained from different Lycopodium species: L. clavatum, L. annotinum, and Huperzia serrata (syn. Lycopodium serratum Thunb.), were tested against Mycobacterium tuberculosis. Dichloromethane and petroleum ether extracts of the mentioned species collected in different geographical sites (in Poland and Ukraine) have shown interesting activities.                Minimal Inhibitory Concentrations (MIC) values for the extracts were determined by a 96-well microplate method with alamarBlue (Invitrogen). The inoculum of the reference strain of Mycobacterium tuberculosis H37Ra in Middlebrook 7H9 broth (Difco) was 5 x 105 cfu/mL per well, according to CLSI standards. Serial twofold dilutions of the extracts ranged from 8 to 256 µg/mL. As the internal control of the method, serial twofold dilutions of four first-line antibiotics dedicated to tuberculosis treatment: isoniazid (INH), rifampicin (RMP), ethambutol (EMB), and streptomycin (SM) were used [1,2]

Synthesis, Characterization and Testing of Antimicrobial Activity of Composites of Unsaturated Polyester Resins with Wood Flour and Silver Nanoparticles

This paper presents the properties of the wood-resin composites. For improving their antibacterial character, silver nanoparticles were incorporated into their structures. The properties of the obtained materials were analyzed in vitro for their anti-biofilm potency in contact with aerobic Gram-positive Staphylococcus aureus and Staphylococcus epidermidis; and aerobic Gram-negative Escherichia coli and Pseudomonas aeruginosa. These pathogens are responsible for various infections, including those associated with healthcare. The effect of silver nanoparticles incorporation on mechanical and thermomechanical properties as well as gloss were investigated for the samples of composites before and after accelerating aging tests. The results show that bacteria can colonize in various wrinkles and cracks on the composites with wood flour but also the surface of the cross-linked unsaturated polyester resin. The addition of nanosilver causes the death of bacteria. It also positively influences mechanical and thermomechanical properties as well as gloss of the resin

Synthesis, Characterization and Testing of Antimicrobial Activity of Composites of Unsaturated Polyester Resins with Wood Flour and Silver Nanoparticles

This paper presents the properties of the wood-resin composites. For improving their antibacterial character, silver nanoparticles were incorporated into their structures. The properties of the obtained materials were analyzed in vitro for their anti-biofilm potency in contact with aerobic Gram-positive Staphylococcus aureus and Staphylococcus epidermidis; and aerobic Gram-negative Escherichia coli and Pseudomonas aeruginosa. These pathogens are responsible for various infections, including those associated with healthcare. The effect of silver nanoparticles incorporation on mechanical and thermomechanical properties as well as gloss were investigated for the samples of composites before and after accelerating aging tests. The results show that bacteria can colonize in various wrinkles and cracks on the composites with wood flour but also the surface of the cross-linked unsaturated polyester resin. The addition of nanosilver causes the death of bacteria. It also positively influences mechanical and thermomechanical properties as well as gloss of the resin