125 research outputs found
Unsteady MHD Boundary Layer Flow of an Incompressible Micropolar Fluid over a Stretching Sheet
Aim of the paper is to investigate the MHD effects on the unsteady boundary layer flow of an incompressible micropolar fluid over a stretching sheet when the sheet is stretched in its own plane. The stretching velocity is assumed to vary linearly with the distance along the sheet. Two equal and opposite forces are impulsively applied along x axis so that the sheet is stretched, keeping the origin fixed in a micropolar fluid. The governing non-linear equations and their associated boundary conditions are first cast into dimensionless form by a local non-similarity transformation. The resulting equations are solved numerically using the Adams- Predictor Corrector method for the
whole transient from the initial state to final steady- state flow. Numerical results are obtained and a representative set
is diaplaced graphically to illustrate the influence of the various physical parameters on the velocity profiles, microrotation profiles as well as the Skin friction coefficient for various values of the material parameter K. It is found that there is a smooth transition from the small- time solution to the large- time solution. Results for the local skin friction coefficient are presented in table as well as in graph
Generation and screening of a comprehensive \u3ci\u3eMycobacterium avium\u3c/i\u3e subsp. \u3ci\u3eparatuberculosis\u3c/i\u3e transposon mutant bank
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne’s Disease in ruminants. This enteritis has significant economic impact and world wide distribution. Vaccination is one of the most cost effective infectious disease control measures. Unfortunately, current vaccines reduce clinical disease and shedding, but are of limited efficacy and do not provide long-term protective immunity. Several strategies have been followed to mine the MAP genome for virulence determinants that could be applied to vaccine and diagnostic assay developent. In this study, a comprehensive mutant bank of 13,536 MAP K-10 Tn5367 mutants (P\u3e95% )was constructed and screened in vitro for phenotypes related to virulence. This strategy was designated to maximize identification of genes important to MAP pathogenesis without relying on studies of other mycobacterial species that may not translate into similar effects in MAP. This bank was screened for mutants with colony morphology alterations, susceptibility to D-cycloserine, impairment in siderophore production or secretion, reduced cell association, and decreased biofilm and clump formation. Mutants with interesting phenotypes were analyzed by PCR, Southern blotting and DNA sequencing to determine transposon insertion sites. These insertion sites mapped up stream from the MAP1152-MAP1156 cluster, internal to either the Mod operon gene MAP1566 or within the coding sequence of lsr2, and several intergenic regions. Growth curves in broth cultures, invasion assays and kinetics of survival and replication in primary bovine macrophages were also determined. The ability of vectors carrying Tn5370 to generate stable MAP mutants was also investigated
Active gas replenishment and sensing of the wetting state in a submerged superhydrophobic surface
Role of water in Protein Aggregation and Amyloid Polymorphism
A variety of neurodegenerative diseases are associated with the formation of
amyloid plaques. Our incomplete understanding of this process underscores the
need to decipher the principles governing protein aggregation. Most
experimental and simulation studies have been interpreted largely from the
perspective of proteins: the role of solvent has been relatively overlooked.
In this Account, we provide a perspective on how interactions with water
affect folding landscapes of A monomers, A oligomer
formation, and protofilament formation in a Sup35 peptide. Simulations show
that the formation of aggregation-prone structures (N) similar to the
structure in the fibril requires overcoming high desolvation barrier. The
mechanism of protofilament formation in a polar Sup35 peptide fragment
illustrates that water dramatically slows down self-assembly. Release of water
trapped in the pores as water wires creates protofilament with a dry interface.
Similarly, one of the main driving force for addition of a solvated monomer to
a preformed fibril is the entropy gain of released water.
We conclude by postulating that two-step model for protein crystallization
must also hold for higher order amyloid structure formation starting from
N. Multiple N structures with varying water content results in a number
of distinct water-laden polymorphic structures. In predominantly hydrophobic
sequences, water accelerates fibril formation. In contrast, water-stabilized
metastable intermediates dramatically slow down fibril growth rates in
hydrophilic sequences.Comment: 27 pages, 4 figures; Accounts of Chemical Research, 201
Purification and characterisation of a carboxylesterase from the latex ofSynadenium grantii Hook, ‘f’
VISUALIZATION OF FLOW THROUGH THE TURBINE BLADE CASCADE WITH OPTIMIZED STREAMWISE BOUNDARY LAYER FENCE
- …