New chemotype of essential oil of Achillea santolina L. collected from different regions of Algeria

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Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium

This study explores the synergistic antibacterial effects of essential oils (EOs) and phenolic extracts from three plants against foodborne pathogenic bacteria. The present work aimed to investigate the synergistic effects of the binary and the ternary combinations of extracts using different blend proportions of the following plant extracts: Artemisia campestris (AC), Artemisia herba alba (AHA), and Citrus aurantium (CA). The antimicrobial activities of EOs and phenolic extracts were determined and evaluated against five strains. For the EOs, the results of the DIZ showed the existence of synergism for different combinations of binary blends, such as AC/AHA or AHA/CA against Escherichia coli, and AC/CA against Enterobacter faecalis. In addition, ternary blends of AC:AHA:CA at a ratio of 1/6:2/3:1/6 exhibited a synergy effect, as measured by the CI, against E. coli. On the other hand, for the phenolic extracts, synergistic effects were noticed for binary blends of AC/CA at different ratios against E. coli, E. faecalis, and Pseudomonas aeruginosa strains. Similarly, ternary blends of phenolic extracts presented synergy against E. coli, E. faecalis, P. aeruginosa strains, and even C. albicans. In this case, the blending ratios were crucial determining factors for maximizing the synergy effect. The study established that the proportion of a single drug could play an essential role in determining the bioefficacy of a drug combination treatment. Therefore, the results showed the importance of studying the modulation of antibacterial activities based on the proportions of extracts in the mixture and finding the range of proportions (as determined by SLMD) that have a synergistic/additive/antagonistic effect with no or low side effects, which can be used in a food preservation system

Essential oils composition of different Achillea santolina L. plant parts growing in Algeria

International audienceThe essential oils (EOs) of leaves, flowers and stems of Achillea santolina L. (Asteraceae) collected at complete flowering stage from Southwest of Algeria, were isolated by hydrodistillation and subsequently analyzed by means of GC and GC/MS. Quantitative and qualitative differences in chemical compositions between the studied parts of this plant were observed. The EOs were rich in oxygenated monoterpenes (65.91–79.94%). The major constituents in the flowers, leaves and stems were: camphor (68.12, 65.17, 55.72%), 1,8-cineole (8.22, 4.77, 0.7%) and α-terpineol (2.84, 5.35, 2.76%). The highest EO yields were obtained for the leaves and the flowers (0.59 and 0.49% “v/w”, respectively), whereas, the stems were characterized by very weak yield value (0.05%)

Chemical Composition of the Essential Oil of Pituranthos scoparius

International audienceThe essential oils obtained by hydro distillation of the aerial parts of Pituranthos scoparius were collected from different regions of Algeria and gave various yields ranging from 0.6 up to 2.8% (v/w). These samples were analyzed by GC and GC-MS showing the occurrence of monoterpene hydrocarbons and some oxygenated compounds. The major components were alpha-pinene (4.4-35.8%), limonene (0.8-66.5%), bornyl acetate (tr-9.6%), myristicin (tr-31.1) and dill apiole (0.4-47.3%). The aerial parts of P. scoparius could be either very rich or very poor in limonene, myristicin or dill apiole. Cluster analysis shows differences in essential oil compositions of samples coming from the different area collected

Send Orders for Reprints to [email protected] Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Three Algerian Lamiaceae Species

International audienceBackground: The present work investigates the chemical composition, the antioxidant and the antimicrobial activities of the Essential Oils (EOs) of three species of the Lamiaceae family gowning in Algeria: Thymus vulgaris L., Thymus algeriensis Boiss. & Reut. and Mentha pulegium L. Methods: Essential Oils (EOs) obtained by hydrodistillation of the aerial parts of the studied plants were analyzed by GC and GC-MS. The antioxidant activity of the EOs was determined using two different assays: free radical scavenging activity of DPPH • (2,2-diphenyl-1-picrylhydrazyl) and Phosphomolybdenum reducing power. The EOs were also tested for their antibacterial and antifungal activities against eight pathogenic bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Methicillin resistant strain of S. aureus " MRSA " and Enterococcus faecalis); a yeast (Candida albicans); and a fungi (Fusarium oxysporum)

Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Three Algerian Lamiaceae Species

International audienceCITATIONS 0 READS 240 5 authors, including: Some of the authors of this publication are also working on these related projects: lipase inhibition: obesity treatment View project how the different properties structural influence in chelation of iron as dipole moment or lipophilicity View project Nadhir Gourine Université Amar Telidji Laghouat Abstract: Background: The present work investigates the chemical composition, the antioxidant and the antimicrobial activities of the Essential Oils (EOs) of three species of the Lamiaceae family gowning in Algeria: Thymus vulgaris L., Thymus algeriensis Boiss. & Reut. and Mentha pulegium L. Methods: Essential Oils (EOs) obtained by hydrodistillation of the aerial parts of the studied plants were analyzed by GC and GC-MS. The antioxidant activity of the EOs was determined using two different assays: free radical scavenging activity of DPPH • (2,2-diphenyl-1-picrylhydrazyl) and Phosphomolybdenum reducing power. The EOs were also tested for their antibacterial and antifungal activities against eight pathogenic bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Methicillin resistant strain of S. aureus " MRSA " and Enterococcus faecalis); a yeast (Candida albicans); and a fungi (Fusarium oxysporum)

Lipid Classes, Fatty Acids, Tocopherols Compositions and Antioxidant Activity of Lawsonia alba Seed Oils Growing in Algeria

International audienceBackground: Lawsonia alba seed oils are not widely used commercially even though they have characteristics that well suit for industrial applications and can contribute to healthy human diets. The most important aim of the present work is the evaluation of the tocopherols composition of these oils. Moreover, lipid classes, fatty acids compositions and antioxidant activity of the seed oils were also investigated. Methods: Lawsonia alba seed oils were extracted with Soxhlet device using n-hexane and chloroform/methanol. Tocopherols were analyzed by HPLC The antioxidant activity were determined using two different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl), and Phosphomoly-bdenum. Results: The content of the oils ranged from 7.52 to 10.45%. Polyunsaturated fatty acids dominated all the studied samples. Linoleic, followed by palmitic and oleic, were the major fatty acids found in the crude seed oils and their lipid classes. Results of tocopherols identification showed that the seed oils were rich in tocopherols, especially-and-tocopherols. For DPPH assay, the antioxidant powers varied from medium to low values (IC 50 = 0.11 to 16.54 mg/mL). The results of the Phosphomolybdenum assay showed very important activities of the oils that were decreasing in the following order: polar lipids > neutral lipids (Chloroform/Methanol) > total lipids > neutral lipids (n-hexane). Conclusion: this current report reveals the richness of the Lawsonia alba seeds in tocopherols, which in fact is studied for the first time. Moreover, cluster analysis revealed that fatty acid composition of the seed oils of Lawsonia alba were very close to those of Walnut

Synergistic antinociceptive activity of combined aqueous extracts of <i>Artemisia campestris</i> and <i>Artemisia herba</i>-<i>alba</i> in several acute pain models

<p>In this study, total phenolic and flavonoid contents, acute toxicity and the antinociceptive activity of <i>Artemisia campestris</i> and <i>Artemisia herba</i>-<i>alba</i>, individually and in combination, were investigated using multiple forms of pain in animals. Our results have been shown that plants are relatively safe without clinical signs of toxicity in animals. Thus, extracts were presented high levels in phenolic and flavonoid contents. <i>Artemisia</i> decoctions with 100, 200, 400 mg/kg b-w studied dose, clearly attenuate chemical and thermal noxious stimuli in writhing, formalin and hot-plate tests, and significantly reduced paw oedema in formalin test. Additionally, binary combination forms exhibited a great improvement in intensity and amplitude of antinociceptive activity in comparison with both plants used individually by a relative interference with opioid system. Our findings suggested the central and peripheral analgesic properties and confirmed the folkloric medicinal use of these plants in pain symptom treatment.</p

Chemical characterization and in vitro antioxidant capacity of nine Algerian date palm cultivars (Phoenix dactylifera L.) seed oil

International audienceDate pits are another major date palm crops and low grade rejected, used locally in the management of diabetes and as animal feed ingredient or turned into non-caffeinated coffee by the Arabs although their richness with valuable bioactive and antioxidant compounds. In this study and for the first time, lipids, fatty acids profiles, total tocopherols and sterols of nine (9) Algerian date palm cultivars (Phoenix dactylifera L.) seed oil were investigated and their antioxidant activities were evaluated. Oil yield ranged from 4.86% to 6.7%. Gas liquid chromatography revealed that the oil fraction contained unsaturated fatty acids in majority with the preponderance of oleic acid (37.83-55.00%) followed by lauric and myristic acids (6.63-25.36% and 9.30-19.33%) respectively. The study of the unsaponifiable fraction revealed the presence of sterols and tocopherols. The sterols contents ranged from 4.7 to 8.45 mg/g DW. Whereas, the levels of total tocopherols ranged from 0.32 to 0.74 mg/DW, which-tocopherol was the predominant tocopherol (15.57%-74.1%) followed by (+)-tocopherol (25.12%-56.01%). The antioxidant activity of date seeds oil was determined with DPPH radical scavenging assays. The oils had a moderate bleaching effect on DPPH free radicals, where Ghars (GH), Sbo3 Lossif (SL) and Timjhourt (TIM) seed oils were the active ones. The richest of date seed oil by active components such sterols, tocopherols also high amounts of oleic, lauric and other fatty acids suggest it to be as a useful source of edible oils for human consumption as well as using as additives in food, for medicinal use, in cosmetics, pharmaceuticals and food industries