Stable Mutated tau441 Transfected SH-SY5Y Cells as Screening Tool for Alzheimer’s Disease Drug Candidates

The role of hyperphosphorylation of the microtubule-associated protein tau in the pathological processes of several neurodegenerative diseases is becoming better understood. Consequently, development of new compounds capable of preventing tau hyperphosphorylation is an increasingly hot topic. For this reason, dependable in vitro and in vivo models that reflect tau hyperphosphorylation in human diseases are needed. In this study, we generated and validated an in vitro model appropriate to test potential curative and preventive compound effects on tau phosphorylation. For this purpose, a stably transfected SH-SY5Y cell line was constructed over-expressing mutant human tau441 (SH-SY5Y-TMHT441). Analyses of expression levels and tau phosphorylation status in untreated cells confirmed relevance to human diseases. Subsequently, the effect of different established kinase inhibitors on tau phosphorylation (e.g., residues Thr231, Thr181, and Ser396) was examined. It was shown with several methods including immunosorbent assays and mass spectrometry that the phosphorylation pattern of tau in SH-SY5Y-TMHT441 cells can be reliably modulated by these compounds, specifically targeting JNK, GSK-3, CDK1/5, and CK1. These four protein kinases are known to be involved in in vivo tau phosphorylation and are therefore authentic indicators for the suitability of this new cell culture model for tauopathies

The SIB Swiss Institute of Bioinformatics' resources: focus on curated databases

The SIB Swiss Institute of Bioinformatics (www.isb-sib.ch) provides world-class bioinformatics databases, software tools, services and training to the international life science community in academia and industry. These solutions allow life scientists to turn the exponentially growing amount of data into knowledge. Here, we provide an overview of SIB's resources and competence areas, with a strong focus on curated databases and SIB's most popular and widely used resources. In particular, SIB's Bioinformatics resource portal ExPASy features over 150 resources, including UniProtKB/Swiss-Prot, ENZYME, PROSITE, neXtProt, STRING, UniCarbKB, SugarBindDB, SwissRegulon, EPD, arrayMap, Bgee, SWISS-MODEL Repository, OMA, OrthoDB and other databases, which are briefly described in this article

The conservation of neurotrophic factors during vertebrate evolution

No abstract availabl

Deixis, Anapher und Katapher und Verwandtes im Grammatischen System des Turkeitürkischen

Donated by Klaus KreiserReprinted from : Zeitschrift der Deutschen Morgenlandischen Gesellschaft Supplement III, 2 / XIX. Deutscher Orientalistentag vom 28. September bis 4. Oktober Vortrage (Wiesbaderi, 1977)

Vollkommene Scheich" (Mürsid-i Kâmil) nach dem İrsadname von Haqiqi.

Donated by Klaus KreiserReprinted from : ARCHIVUM OTTOMANICUM 20 (2002), Harrassowitz, Verlag

Prof Dr. Manfred, Götz.

Donated by Klaus KreiserReprinted from : Ankara : Ataturk Kultur, Dil ve Tarih Yüksek Kurumu Bülteni, Sayı 20, Cilt VII, 1994

A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p

No abstract availabl

Isolation and characterization of cold-shock domain protein genes, Oryzias latipes Y-box protein 2 (OlaYP2) and Fugu rubripes Y-box protein 1 (FruYP1), in medakafish and pufferfish

Copyright © 2002 Elsevier Science B.V. All rights reserved.Enchshargal Zend-Ajusch, Ute Hornung, Carola Burgtorf, Götz Lütjens, Zhihong Shan, Manfred Schartl and Thomas Haafhttp://www.elsevier.com/wps/find/journaldescription.cws_home/506033/description#descriptio

Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone

Addition of increasing doses of synthetic growth hormone-release inhibiting hormone (GH-RIH) leads to a progressive decrease of the basal and N 6monobutyryl cyclic AMP-,theophylline- and prostaglandin E 2-induced release of immunoreactive growth hormone (GH) and thyrotropin (TSH) release from rat anterior pituitary cells in monolayer culture. A halfmaximal effect is measured at 3 × 10 −9 M GH-RIH while a maximal inhibition to 10–20% of the control level is found at 1 × 10 −7 M. Using rat hemipituitaries and measurement of GH release by both polyacrylamide gel electrophoresis and radioimmunoassay, a maximal effect of GH-RIH was found in the first 5 min of incubation. The inhibitory effect of GH-RIH on GH release remained constant for at least 3 h. GH-RIH does not affect the basal or induced release of prolactin and luteinizing hormone nor the high K +-induced release of GH and TSH