26 research outputs found
Transcriptomic analysis supports similar functional roles for the two thymuses of the tammar wallaby
Background: The thymus plays a critical role in the development and maturation of T-cells. Humans have a single thoracic thymus and presence of a second thymus is considered an anomaly. However, many vertebrates have multiple thymuses. The tammar wallaby has two thymuses: a thoracic thymus (typically found in all mammals) and a dominant cervical thymus. Researchers have known about the presence of the two wallaby thymuses since the 1800s, but no genome-wide research has been carried out into possible functional differences between the two thymic tissues. Here, we used pyrosequencing to compare the transcriptomes of a cervical and thoracic thymus from a single 178 day old tammar wallaby.Results: We show that both the tammar thoracic and the cervical thymuses displayed gene expression profiles consistent with roles in T-cell development. Both thymuses expressed genes that mediate distinct phases of T-cells differentiation, including the initial commitment of blood stem cells to the T-lineage, the generation of T-cell receptor diversity and development of thymic epithelial cells. Crucial immune genes, such as chemokines were also present. Comparable patterns of expression of non-coding RNAs were seen. 67 genes differentially expressed between the two thymuses were detected, and the possible significance of these results are discussed.Conclusion: This is the first study comparing the transcriptomes of two thymuses from a single individual. Our finding supports that both thymuses are functionally equivalent and drive T-cell development. These results are an important first step in the understanding of the genetic processes that govern marsupial immunity, and also allow us to begin to trace the evolution of the mammalian immune system
Gatekeeper of pluripotency: A common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells
A comprehensive, time-resolved SANS investigation of temperature-change-induced sponge-to-lamellar and lamellar-to-sponge phase transformations in comparison with
Time-resolved small-angle neutron scattering (TR-SANS) was employed to observe temperature-induced phase transitions from the sponge (L
3 to the lamellar ( L
α phase, and vice versa, in the water-oil (n -decane)-non-ionic surfactant ( C12E5 system using both bulk and film contrast. Samples of different bilayer volume fractions φ and solvent viscosities η were investigated applying various amplitudes of temperature jump ΔT . The findings of a previous 2H -NMR study could be confirmed, where the lamellar phase formation was determined to occur through a nucleation and growth process, while it was concluded that the L
3 -phase develops in a mechanistically different and more rapid manner involving uncorrelated passage formation. Likewise, the kinetic trends of the nucleation and growth transition (decreased transition time with increase of φ and ΔT were witnessed once again. Additionally, NMR and SANS data that demonstrate a strong dependency of that process on solvent viscosity η are presented. Contrariwise, it is made evident via both SANS and NMR results that the L
α -to-L
3 transition time is independent (within experimental sensitivity) of the varied parameters (φ , ΔT , η . Unusual scattering evolution in one experiment, originating from a highly ordered lamellar phase, intriguingly hints that a major rate determining factor is the disruption of long-range order. Furthermore, the bulk contrast investigations give insight into structure peak shifts/development during the transitions, while the film contrast experiments prove the bilayer thickness to be constant throughout the phase transitions and show that there is no evidence for a change in the short-range order of the bilayer structure. The latter was considered possible, due to the different topology of the L
3 and L
α phases. Lastly, an unexpected yet consistent appearance of anisotropic scattering is detected in the L
3 -to- L
α transitions
A comprehensive, time-resolved SANS investigation of temperature-change-induced sponge-to-lamellar and lamellar-to-sponge phase transformations in comparison with 2H -NMR results
Time-resolved small-angle neutron scattering (TR-SANS) was employed to observe temperature-induced phase transitions from the sponge (L-3 to the lamellar ( L (alpha) phase, and vice versa, in the water-oil (n-decane)-non-ionic surfactant (C12E5 system using both bulk and film contrast. Samples of different bilayer volume fractions phi and solvent viscosities eta were investigated applying various amplitudes of temperature jump Delta T . The findings of a previous H-2-NMR study could be confirmed, where the lamellar phase formation was determined to occur through a nucleation and growth process, while it was concluded that the L-3-phase develops in a mechanistically different and more rapid manner involving uncorrelated passage formation. Likewise, the kinetic trends of the nucleation and growth transition (decreased transition time with increase of phi and Delta T were witnessed once again. Additionally, NMR and SANS data that demonstrate a strong dependency of that process on solvent viscosity eta are presented. Contrariwise, it is made evident via both SANS and NMR results that the L-alpha-to-L-3 transition time is independent (within experimental sensitivity) of the varied parameters (phi, Delta T, eta) . Unusual scattering evolution in one experiment, originating from a highly ordered lamellar phase, intriguingly hints that a major rate determining factor is the disruption of long-range order. Furthermore, the bulk contrast investigations give insight into structure peak shifts/development during the transitions, while the film contrast experiments prove the bilayer thickness to be constant throughout the phase transitions and show that there is no evidence for a change in the short-range order of the bilayer structure. The latter was considered possible, due to the different topology of the L-3 and L-alpha phases. Lastly, an unexpected yet consistent appearance of anisotropic scattering is detected in the L-3-to-L-alpha transitions