An early evaluation of translocation actions for endangered plant species on Mediterranean islands

In situ conservation is widely considered a primary conservation strategy. Plant translocation, specifically, represents an important tool for reducing the extinction risk of threatened species. However, thus far, few documented translocations have been carried out in the Mediterranean islands. The Care-Mediflora project, carried out on six Mediterranean islands, tackles both short- and long-term needs for the insular endangered plants through in situ and ex situ conservation actions. The project approach is based on using ex situ activities as a tool to improve in situ conservation of threatened plant species. Fifty island plants (representing 45 taxa)were selected for translocations using common criteria. During the translocations, several approaches were used, which differed in site selection method, origin of genetic material, type of propagative material, planting method, and more. Although only preliminary data are available, some general lessons can be learned from the experience of the Care-Mediflora project. Among the factors restricting the implementation of translocations, limited financial resources appear to be the most important. Specific preliminary management actions, sometimes to be reiterated after translocation, increase the overall cost, but often are necessary for translocation success. Translocation using juvenile/reproductive plants produces better results over the short term, although seeds may provide good results over the long run (to be assessed in the future). Regardless, plant translocation success can only be detected over long periods; therefore, proper evaluation of plant translocations requires a long-term monitoring protocol. Care-Mediflora project represents the first attempt to combine the existing approaches in a common plant conservation strategy specifically focusing on the Mediterranean islands

A common approach to the conservation of threatened island vascular plants: First results in the mediterranean basin

The Mediterranean islands represent a center of vascular plant diversity featuring a high rate of endemic richness. Such richness is highly threatened, however, with many plants facing the risk of extinction and in need of urgent protection measures. The CARE-MEDIFLORA project promoted the use of ex situ collections to experiment with in situ active actions for threatened plants. Based on common criteria, a priority list of target plant species was elaborated, and germplasm conservation, curation and storage in seed banks was carried out. Accessions were duplicated in the seed banks of the partners or other institutions. Germination experiments were carried out on a selected group of threatened species. A total of 740 accessions from 429 vascular plants were stored in seed banks, and 410 seed germination experiments for 283 plants species were completed; a total of 63 in situ conservation actions were implemented, adopting different methodological protocols. For each conservation program, a specific monitoring protocol was implemented in collaboration with local and regional authorities. This project represents the first attempt to develop common strategies and an opportunity to join methods and methodologies focused on the conservation of threatened plants in unique natural laboratories such as the Mediterranean islands

Novel quality control methods in conjunction with chemometrics (multivariate analysis) for detecting honey authenticity

The importance of honey has been recently upgraded because of its nutrient and therapeutic effect. The adulteration of honey increased exponentially in terms of both geographic and/or botanical origin. Therefore, the need has arisen for more effective quality control methods aiming at detecting adulteration. Various novel, fast, and accurate methods like AAS, HPLC, GC-MS, ES-MS, TLC, HPAED-PAD, NMR, FT-Raman, and NIR have enriched the arsenal of analytical chemist in this direction. However, apart from these novel methods, the application of multivariate analysis and, in particular PCA, CLA, and CA, proved to be extremely useful for grouping and detecting honey of various origins. Mineral and trace element analysis were repeatedly shown to be a very effective means for the classification purposes of honey of various origins (geographical and botanical)

Free behind bars: Effects of browsing exclusion on the growth and regeneration of Zelkova abelicea

Caprine overbrowsing is a main threat to trees of pastoral mountain woodlands in the Mediterranean region of Europe. In this study, we assess the impact of browsing exclusion on the growth and regeneration of the endangered Zelkova abelicea, a Paleogene relict tree endemic to Crete (Greece). Shoot elongation, height growth and natural regeneration were monitored during two to five years in 31 fenced plots across the distribution range of the species. We found that in the absence of browsing pressure, Z. abelicea produces shoots on average two times longer than when browsed, therefore clearly demonstrating the negative impact of current browsing pressure on the growth performance of the species. Shoot elongation and height growth were maximal in most plots during the first two years following fencing, after which growth rates decreased. The proportion of trees that reached a height considered out of reach of caprine browsing had more than doubled already after four years, although this was dependent on initial tree height. Shoot elongation and height growth were positively correlated with tree height, and smaller trees took more time to reach a specific height. Seedlings were only found in three fenced plots as well as in two control browsed areas during a single year and only in western Crete. This underlines the possible strong and concerning impact of climatic factors on seed formation as well as on seedling growth and survival, although site specificities such as stand structure and micro-topography should also be considered. Our findings have important implications for conservation actions, as they can help choose which stands and which individuals to fence in priority for efficient long-term conservation. © 2021 The Author

Physicochemical Characterization and Biological Properties of Pine Honey Produced across Greece

Pine honey is a honeydew honey produced in the East Mediterranean region (Greece and Turkey) from the secretions of the plant sucking insect Marchalina hellenica (Gennadius) (Coccoidea: Marchalini-dae) feeding on living parts of Pinus species. Nowadays, honeydew honey has attracted great attention due to its biological activities. The aim of this study was to study unifloral pine honey samples produced in Greece regarding their physicochemical parameters and antioxidant and antibacterial activity against five nosocomial and foodborne pathogens. These honeys showed physicochemical and microscopic characteristics within the legal limits, except for diastase activity, a parameter known to be highly variable, depending on various factors. Substantially higher levels of H2 O2 were estimated compared to other types of honeydew honey, whereas protein content was similar. The total phenolic content was 451.38 ± 120.38 mg GAE/kg and antiradical activity ranged from 42.43 to 79.33%, while FRAP values (1.87 to 9.43 mmol Fe+2 /kg) were in general higher than those reported in the literature. Various correlations could be identified among these parameters. This is the first attempt to investigate in depth the antibacterial activity of pine honey from Greece and correlate it with honey quality parameters. All tested honeys exerted variable but significant antibacterial activity, expressed as MIC and MBC values, comparable or even superior to manuka honey for some tested samples. Although honey antibacterial activity is mainly attributed to hydrogen peroxide and proteins in some cases (demonstrated by elevated MICs after catalase and Proteinase K treatment, respectively), no strong correlation between the antibacterial activity and hydrogen peroxide concentration or total protein content was demonstrated in this study. However, there was a statistically significant correlation of moisture, antioxidant and antibacterial activity against Klebsiella pneuomoniae, as well as antioxidant and antibacterial activity against Salmonella ser. Typhimurium. Interestingly, a statistically significant negative correlation has been observed between. © 2022 by the authors. Licensee MDPI, Basel, Switzerland