235 research outputs found
Practical use with the computer in Arithmetic and Mathematics
この論文では,算数・数学におけるコンピュータ使用法の利点と問題点を考察するため,鳥取市内の小中学校の児童生徒にアンケートを行った。その結果,コンピュータの使われ方とともに算数・数学の弱いと思われる分野が明らかになった。また,算数・数学への効果的な利用法を提案した
<Abstract of Published Report>Structural and Spectral Characteristics of the Electrogenerated Tetracyanoethylene Dianion.
<Abstract of Published Report>Non-aqueous Capillary Electrophoresis of p-Quinone Anion Radicals.
<Abstract of Published Report>n-σCharge-Transfer Interaction and Molecular and Electronic Structural Properties in the Hydrogen-Bonding Systems Consisting of p-Quinone Dianions and Methyl Alcohol.
Development of a Vertex Finding Algorithm using Recurrent Neural Network
Deep learning is a rapidly-evolving technology with possibility to
significantly improve physics reach of collider experiments. In this study we
developed a novel algorithm of vertex finding for future lepton colliders such
as the International Linear Collider. We deploy two networks; one is simple
fully-connected layers to look for vertex seeds from track pairs, and the other
is a customized Recurrent Neural Network with an attention mechanism and an
encoder-decoder structure to associate tracks to the vertex seeds. The
performance of the vertex finder is compared with the standard ILC
reconstruction algorithm.Comment: 8 pages, 8 figures, preliminary version currently under review by IL
The phagocytosis oxidase/Bem1p domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity
Perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors activates RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) through direct phosphorylation by BOTRYTIS-INDUCED KINASE 1 (BIK1) and induces the production of reactive oxygen species (ROS). RBOHD activity must be tightly controlled to avoid the detrimental effects of ROS, but little is known about RBOHD downregulation. To understand the regulation of RBOHD, we used co-immunoprecipitation of RBOHD with mass spectrometry analysis and identified PHAGOCYTOSIS OXIDASE/BEM1P (PB1) DOMAIN-CONTAINING PROTEIN (PB1CP). PB1CP negatively regulates RBOHD and the resistance against the fungal pathogen Colletotrichum higginsianum. PB1CP competes with BIK1 for binding to RBOHD in vitro. Furthermore, PAMP treatment enhances the PB1CP-RBOHD interaction, thereby leading to the dissociation of phosphorylated BIK1 from RBOHD in vivo. PB1CP localizes at the cell periphery and PAMP treatment induces relocalization of PB1CP and RBOHD to the same small endomembrane compartments. Additionally, overexpression of PB1CP in Arabidopsis leads to a reduction in the abundance of RBOHD protein, suggesting the possible involvement of PB1CP in RBOHD endocytosis. We found PB1CP, a novel negative regulator of RBOHD, and revealed its possible regulatory mechanisms involving the removal of phosphorylated BIK1 from RBOHD and the promotion of RBOHD endocytosis
<Abstract of published report>Spectroscopic Study of the Conformational Dependence on Acid Dissociation of Phenoxypyridinium Cations.
Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells
Embryonic stem cells (ESCs) could potentially compensate for the lack of blood platelets available for use in transfusions. Here, we describe a new method for generating mouse ESC-derived platelets (ESPs) that can contribute to hemostasis in vivo. Flow cytometric sorting of cells from embryoid bodies on day 6 demonstrated that c-Kit+ integrin αIIb (αIIb)+ cells, but not CD31+ cells or vascular endothelial cadherin+ cells, are capable of megakaryopoiesis and the release of platelet-like structures by day 12. αIIbβ3-expressing ESPs exhibited ectodomain shedding of glycoprotein (GP)Ibα, GPV, and GPVI, but not αIIbβ3 or GPIbβ. ESPs showed impaired αIIbβ3 activation and integrin-mediated actin reorganization, critical events for normal platelet function. However, the administration of metalloproteinase inhibitors GM6001 or TAPI-1 during differentiation increased the expression of GPIbα, improving both thrombogenesis in vitro and posttransfusion recovery in vivo. Thus, the regulation of metalloproteinases in culture could be useful for obtaining high-quality, efficacious ESPs as an alternative platelet source for transfusions
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