Costs of Chronic Waterborne Zinc Exposure and the Consequences of Zinc Acclimation on the Gill/Zinc Interactions of Rainbow Trout in Hard and Soft Water

Juvenile rainbow trout were exposed to zinc in both moderately hard water (hardness 5 120 mg CaCO3/L, pH = 8.0, Zn = 150 μg/L or 450 μg/L) and soft water (hardness = 20 mg CaCO3/L, pH = 7.2, Zn = 50 μg/L or 120 μg/L) for 30 d. Only the 450 mg/L zinc–exposed fish experienced significant mortality (24% in the first 2 d). Zinc exposure caused no effect on growth rate, but growth affected tissue zinc levels. Whole body zinc levels were elevated, but gills and liver showed no consistent increases relative to controls over the 30-d. Therefore, tissue zinc residues were not a good indicator of chronic zinc exposure. After the 30-d exposure, physiological function tests were performed. Zinc was 5.4 times more toxic in soft water (control 96 h LC50s in hard and soft water were 869 μg/L and 162 μg/L, respectively). All zinc-exposed trout had acclimated to the metal, as seen by an increase in the LC50 of 2.2 to 3.9 times over that seen in control fish. Physiological costs related to acclimation appeared to be few. Zinc exposure had no effect on whole body Ca2+ or Na+ levels, on resting or routine metabolic rates, or on fixed velocity sprint performance. However, critical swimming speed (UCrit) was significantly reduced in zinc-exposed fish, an effect that persisted in zinc-free water. Using radioisotopic techniques to distinguish new zinc incorporation, the gills were found to possess two zinc pools: a fast turnover pool (T1/2 = 3–4 h) and a slow turnover pool (T1/2 = days to months). The fast pool was much larger in soft water than in hard water, but at most it accounted for \u3c3.5% of the zinc content of the gills. The size of the slow pool was unknown, but its loading rate was faster in soft water. Chronic zinc exposure was found to increase the size of the fast pool and to increase the loading rate of the slow pool

Correspondence regarding 'Clouded leopards, the secretive top-carnivore of South-East Asian rainforests: their distribution, status and conservation needs in Sabah, Malaysia'

Correspondence regarding Wilting A, Fischer F, Bakar SA, Linsenmair KE: Clouded leopards, the secretive top-carnivore of South-East Asian rainforests: their distribution, status and conservation needs in Sabah, Malaysia. BMC Ecol 2006, 6:16

An Approach for Planning Sensor-Based Inspection of the Built Environment

The promise of lower costs for sensors that can be used for construction inspection means that inspectors will continue to have new choices to consider in creating inspection plans. However, these emerging inspection methods can require different activities, resources, and decisions such that it can be difficult to compare the emerging methods with other methods that satisfy the same inspection needs. Furthermore, the context in which inspection is performed can significantly influence how well certain inspection methods are suited for a given set of goals for inspection. Context information, such as weather, security, and the regulatory environment, can be used to understand what information about a component should be collected and how an inspection should be performed. The research described in this paper is aimed at developing an approach for comparing and selecting inspection plans. This approach consists of (1) refinement of given goals for inspection, if necessary, in order to address any additional information needs due to a given context and in order to reach a level of detail that can be addressed by an inspection activity; (2) development of constraints to describe how an inspection should be achieved; (3) matching of goals to available inspection methods, and generation of activities and resource plans in order to address the goals; and (4) selection of an inspection plan from among the possible plans that have been identified. The authors illustrate this approach with observations made at a local construction site

Planetary Collisions outside the Solar System: Time Domain Characterization of Extreme Debris Disks

Luminous debris disks of warm dust in the terrestrial planet zones around solar-like stars are recently found to vary, indicative of ongoing large-scale collisions of rocky objects. We use Spitzer 3.6 and 4.5 {\mu}m time-series observations in 2012 and 2013 (extended to 2014 in one case) to monitor 5 more debris disks with unusually high fractional luminosities ("extreme debris disk"), including P1121 in the open cluster M47 (80 Myr), HD 15407A in the AB Dor moving group (80 Myr), HD 23514 in the Pleiades (120 Myr), HD 145263 in the Upper Sco Association (10 Myr), and the field star BD+20 307 (>1 Gyr). Together with the published results for ID8 in NGC 2547 (35 Myr), this makes the first systematic time-domain investigation of planetary impacts outside the solar system. Significant variations with timescales shorter than a year are detected in five out of the six extreme debris disks we have monitored. However, different systems show diverse sets of characteristics in the time domain, including long-term decay or growth, disk temperature variations, and possible periodicity.Comment: 50 pages, 14 figures, 9 tables; Accepted for publication in the Astrophysical Journa

Differential effects of nutritional folic acid deficiency and moderate hyperhomocysteinemia on aortic plaque formation and genome-wide DNA methylation in vascular tissue from ApoE-/- mice

Low folate intake is associated with vascular disease. Causality has been attributed to hyperhomocysteinemia. However, human intervention trials have failed to show the benefit of homocysteine-lowering therapies. Alternatively, low folate may promote vascular disease by deregulating DNA methylation. We investigated whether folate could alter DNA methylation and atherosclerosis in ApoE null mice. Mice were fed one of six diets (n = 20 per group) for 16 weeks. Basal diets were either control (C; 4% lard) or high fat (HF; 21% lard and cholesterol, 0.15%) with different B-vitamin compositions: (1) folic acid and B-vitamin replete, (2) folic acid deficient (−F), (3) folic acid, B6 and B12 deficient (−F−B). −F diets decreased plasma (up to 85%; P < 0.05), whole blood (up to 70%; P < 0.05), and liver folate (up to 65%; P < 0.05) and hepatic SAM/SAH (up to 80%; P < 0.05). −F−B diets reduced plasma (up to 76%; P < 0.05), whole blood (up to 72%; P < 0.05), and liver B12 (up to 39%; P < 0.05) and hepatic SAM/SAH (up to 90%; P < 0.05). −F increased homocysteine 2-fold, while −F−B increased homocysteine 3.6- and 6.8-fold in the C and HF groups (P < 0.05). Plaque formation was increased 2-fold (P < 0.0001) in mice fed a HF diet. Feeding a HF–F diet increased lesion formation by 17% (P < 0.05). There was no change in 5-methyldeoxycytidine in liver or vascular tissue (aorta, periadventitial tissue and heart). These data suggest that atherogenesis is not associated with genome-wide epigenetic changes in this animal model

The estimated burden of fungal disease in South Africa

Publication fees were paid via funding from a grant from Fonds.Peer reviewedPublisher PD

MOST photometry of the RRd Lyrae variable AQ Leo: Two radial modes, 32 combination frequencies, and beyond

Highly precise and nearly uninterrupted optical photometry of the RR Lyrae star AQ Leo was obtained with the MOST (Microvariability & Oscillations of STars) satellite over 34.4 days in February-March 2005. AQ Leo was the first known double-mode RR Lyrae pulsator (RRd star). Three decades after its discovery, MOST observations have revealed that AQ Leo oscillates with at least 42 frequencies, of which 32 are linear combinations (up to the sixth order) of the radial fundamental mode and its first overtone. Evidence for period changes of these modes is found in the data. The other intrinsic frequencies may represent an additional nonradial pulsation mode and its harmonics (plus linear combinations) which warrant theoretical modeling. The unprecedented number of frequencies detected with amplitudes down to millimag precision also presents an opportunity to test nonlinear theories of mode growth and saturation in RR Lyrae pulsators.Comment: accepted for publication in MNRAS; revision v2 : broken references have been fixe

Deep Imaging of AXJ2019+112: The Luminosity of a ``Dark Cluster''

We detect a distant cluster of galaxies centered on the QSO lens and luminous X-ray source AXJ2019+112, a.k.a. ``The Dark Cluster'' (Hattori et al 1997). Using deep V,I Keck images and wide-field K_s imaging from the NTT, a tight red sequence of galaxies is identified within a radius of 0.2 h^{-1} Mpc of the known z=1.01 elliptical lensing galaxy. The sequence, which includes the central elliptical galaxy, has a slope in good agreement with the model predictions of Kodama et al (1998) for z~1. We estimate the integrated rest-frame luminosity of the cluster to be L_V > 3.2 x 10^{11}h^{-2}L_{\sun} (after accounting for significant extinction at the low latitude of this field), more than an order of magnitude higher than previous estimates. The central region of the cluster is deconvolved using the technique of Magain, Courbin & Sohy (1998), revealing a thick central arc coincident with an extended radio source. All the observed lensing features are readily explained by differential magnification of a radio loud AGN by a shallow elliptical potential. The QSO must lie just outside the diamond caustic, producing two images, and the arc is a highly magnified image formed from a region close to the center of the host galaxy, projecting inside the caustic. The mass--to--light ratio within an aperture of 0.4 h ^{-1} Mpc is M_x/L_V= 224^{+112}_{-78}h(M/L_V)_{\sun}, using the X-ray temperature. The strong lens model yields a compatible value, M/L_V= 372^{+94}_{-94}h(M/L_V)_{\sun}, whereas an independent weak lensing analysis sets an upper limit of M/L_V <520 h(M/L_V)_{\sun}, typical of massive clusters.Comment: AAS Latex format, 24 pages, 9 figures. Fig 1a,b available at http://astro.berkeley.edu/~benitezn/cluster.html . Submitted to ApJ on August 15t

The FIRST Bright Quasar Survey. II. 60 Nights and 1200 Spectra Later

We have used the VLA FIRST survey and the APM catalog of the POSS-I plates as the basis for constructing a new radio-selected sample of optically bright quasars. This is the first radio-selected sample that is competitive in size with current optically selected quasar surveys. Using only two basic criteria, radio-optical positional coincidence and optical morphology, quasars and BL Lacs can be identified with 60% selection efficiency; the efficiency increases to 70% for objects fainter than magnitude 17. We show that a more sophisticated selection scheme can predict with better than 85% reliability which candidates will turn out to be quasars. This paper presents the second installment of the FIRST Bright Quasar Survey with a catalog of 636 quasars distributed over 2682 square degrees. The quasar sample is characterized and all spectra are displayed. The FBQS detects both radio-loud and radio-quiet quasars out to a redshift z>3. We find a large population of objects of intermediate radio-loudness; there is no evidence in our sample for a bimodal distribution of radio characteristics. The sample includes ~29 broad absorption line quasars, both high and low ionization, and a number of new objects with remarkable optical spectra.Comment: 41 pages plus 39 gifs which contain all quasar spectra. Accepted for publication in the Astrophysical Journal Supplement Serie