Forage grass productivity and quality in south-western part of Pannonian basin

The aim of the research was to compare the productivity and quality of several forage grasses in the climate conditions of south-eastern Europe. The research was conducted during 2012 and 2013, in the south-western part of the Pannonian basin (vicinity of Osijek, Croatia). The experiment included 5 grass species: Italian ryegrass (Lolium multiflorum), cocksfoot (Dactylis glomerata), hybrid ryegrass (Lolium x boucheanum), perennial ryegrass (Lolium perenne) and timothy (Phleum pratense) cut three times per year, i.e. 6 cuttings in total for two years. The results have shown that there were significant statistical differences for all investigated traits between the years of the species and their interaction at the P lt 0.05 levels. In average, the highest dry matter yield was achieved during the first year of usage (10.4 and 9.1 t/ha). The average usage of two years, the highest yields of dry matter had Italian ryegrass and cocksfoot. Quality results showed that the cocksfoot was the least digestible because it contained over 700 g/kg of neutral detergent fiber (NDF) and 450 g/kg of acid detergent fiber (ADF), unlike the perennial ryegrass which had 559 and 327 g/kg of NDF and ADF. Energetically most productive was Italian ryegrass with a production potential of 19,739 liters of milk and with milk fat of 4%. On the protein productivity base, the most prominent was the cocksfoot which could produce 11,878 liters of milk from 713 kg proteins in one year. The results show that none of the tested grasses had a balanced relationship between protein and energy

AGRONOMIC VALUE OF SPRING FIELD PEA BREEDING LINES AND VARIETIES FOR GREEN FORAGE PRODUCTION (Pisum sativum L.)

Jari stočni grašak jedna je od najznačajnijih krupnozrnih mahunarki. Kod većine oplemenjivačkih programa graška selekcijski je proces usmjeren na razvoj visokoprinosnoga i kvalitetnoga sortimenta za proizvodnju zrna i/ili visokokvalitetne voluminozne stočne hrane. Proizvodnja zrna bogatoga bjelančevinama uglavnom je povezana s jarim sortama, dok je proizvodnja voluminozne mase, odnosno korištenje cijele biljke, koja je, osim bjelančevina, značajan izvor minerala i vitamina, odlika ozimih sorti. Zbog nemogućnosti jesenje sjetve (kasno napuštanje polja prethodne kulture, obilne kiše u vrijeme pripreme tla i sjetve, promjena poslovnoga plana, veći broj stoke i sl.), sve su češći zahtjevi proizvođača za jarim sortama graška, koje daju veliku masu u kratkome periodu (100 dana) i omogućuju sjetvu druge kulture na istoj površini. Ciljevi ovoga istraživanja bili su: tijekom dvije uzastopne godine (2012. i 2013.) procijeniti agronomsku vrijednost sorti (jarih, fakultativno ozimih) i novostvorenih oplemenjivačkih linija jaroga stočnoga graška (7 linja, 6 sorti) te odabrati najperspektivnije linije za daljnji selekcijski proces i/ili pokretanje postupka prijave nove potencijalne sorte jaroga graška za proizvodnju voluminoznoga krmiva. Najveće prosječne prinose zelene mase ostvarile su oplemenjivačke linije MBK-7 (33 t ha-1), MBK 41 (28 t ha-1) i MBK-51(28 t ha-1) te sorte Dora (35 t ha-1) i Poneka (34 t ha-1). Navedene sorte predstavljaju vrijedan materijal za daljnji oplemenjivački rad, a identificirane superiorne linije imaju potencijal za nove sorte.Spring field pea is one of the most important coarse legumes. In most pea breeding programs, the selection process is focused on the development of high-yielding and top-quality varieties for grain and/or green forage production. Production of protein rich grain is mainly associated with spring varieties, while production of green mass rich in protein, minerals and vitamins is characteristic for winter varieties. Due to problems with planting in autumn (late harvest of previous crop, heavy rains during soil preparation and planting, business plan modifications, abundance of cattle etc.), farmers often prefer spring pea varieties that can produce large green mass over shorter period of time, as well as ensure planting of the next crop in the same field. Objectives of this research were: agronomic value assessment of (spring and potentially winter) varieties and new breeding lines of spring field pea over a two-year period (2012-2013), as well as the selection of lines with the most potential for further breeding process and/or registration of new spring field varieties for green forage production. The highest mean yields of green mass and dry matter, as well as favourable values of other assessed traits were obtained by breeding lines MBK-7, MBK-41, MBK-51, and varieties Dora and Poneka. These varieties represent valuable germplasm for further breeding process, while selected lines have high potential for the development of new varieties

Influence of growth stimulators on the initial growth of maize hybrids

Tretiranje sjemena kukuruza stimulatorima rasta novija je mjera u procesu dorade i proizvodnje sjemena. Cilj istraživanja bio je utvrditi utjecaj različitih stimulatora rasta na početni porast mladih biljaka kukuruza. Tijekom 2020. godine u Osijeku su provedena istraživanja na tri hibrida kukuruza čije je sjeme tretirano uz mješavinu fungicida i insekticida s još četiri komercijalne varijante tretmana stimulatora rasta. Nakon nicanja provedena su mjerenja visine biljke i mase nadzemnog dijela biljke u vegetacijskoj fazi početnog porasta kukuruza. Rezultati istraživanja pokazali su statistički vrlo značajan utjecaj stimulatora rasta na visinu biljke, a isto tako i na masu nadzemnog dijela biljke. Najveći pozitivan učinak izmjeren je kod hibrida kukuruza OSSK 403 za oba promatrana agronomska svojstva. Primjena stimulatora rasta na sjemenu hibrida kukuruza je vrlo jednostavna u samom postupku dorade te se zbog kasnijeg pozitivnog učinka na početni porast hibrida kukuruza može preporučiti u proizvodnji kukuruza. Pozitivno djelovanje stimulatora rasta na metabolizam biljaka u mnogome pomažu biljci kukuruza prevladavanju negativnih agroekoloških utjecaja.Treatment of corn seeds with growth stimulants is a newer measure in the process of seed processing and production. The aim of the study was to determine the effect of different growth stimulators on the initial growth of young maize plants. The research was conducted on three maize hybrids whose seeds were treated with four commercial variants of growth stimulator treatment. After germination, measurements of plant height and mass of the aboveground part of the plant in the vegetation phase of the initial growth of maize were performed. The results of the research showed a statistically very significant influence of growth stimulators on the height of the plant and also on the mass of the aboveground part of the plant. The greatest positive effect was measured in maize hybrids OSSK 403 for both observed agronomic traits. The application of growth stimulators on maize hybrid seeds is very simple in the finishing process and due to the subsequent positive effect on the initial growth of maize hybrids can be recommended in maize production. The positive effects of growth stimulants on plant metabolism greatly help the maize plant to overcome negative agroecological impacts

POLIFENOLI I NJIHOVA ANTIOKSIDACIJSKA AKTIVNOST U HRVATSKOJ KOLEKCIJI CRVENE DJETELINE

The leaf samples of two varieties, twenty breeding populations, and seven local populations of the Croatian red clover were collected in the full flowering stage, with an aim to evaluate their phenolic content and antioxidant activity by using the spectrophotometric methods. A significant variation among the varieties/populations in the content of total phenolics and flavonoids was determined. The results demonstrated that the red clover is a rich source of phenolics and flavonoids, ranging from 38.67 to 59.96 mg GAE/g of DM and 21.19 to 51.48 mg CE/g of DM, respectively. The high levels in both phenolics and flavonoids were found in breeding populations/variety CD-8, Rc-11/7, Rc-11/8, Rc-11/15 and OS Osiris. The leaf extracts manifested a strong antioxidant activity towards diphenyl-1-picrylhydrazyl (DPPH), with a variation from 31.50 to 63.14%. A significant correlation was found between the antioxidant activity of the extracts and their total phenolic and flavonoid content (r = 0.737 and 0.839, respectively). Considering the obtained results, the crude extracts of red clover manifested a significant antioxidant potential and can be used as a natural source of biologically active components in the human and animal nutrition.Listovi crvene djeteline uzorkovani su u fazi pune cvatnje s dvije sorte, dvadeset oplemenjivačkih populacija i sedam lokalnih populacija crvene djeteline u Hrvatskoj s ciljem određivanja ukupnih fenola i flavonoida i njihove antioksidacijske aktivnosti spektrofotometrijskim metodama. Pronađena je statistički značajna varijabilnost među sortama/populacijama u ukupnome sadržaju fenola i flavonoida. Rezultati su pokazali da je crvena djetelina bogat izvor fenola s rasponom koncentracija od 38,67 do 59,96 mg GAE/g u ST te flavonoida od 21,19 do 51,48 mg CE/g u ST. Visoka koncentracija fenola i flavonoida izmjerena je u oplemenjivačkim populacijama/sortama CD-8, Rc-11/7, Rc-11/8, Rc-11/15 i OS Osiris. Biljni ekstrakt dobiven iz lista crvene djeteline imao je snažnu antioksidacijsku aktivnost prema difenil-1-pikrilhidrazilu (DPPH) s varijabilnošću od 31,50 do 63,14%, koja statistički značajno korelira sa sadržajem ukupnih fenola (r = 0,737) i flavonoida (r = 0,839). Dobiveni rezultati pokazuju da sirovi ekstrakti crvene djeteline imaju značajan antioksidacijski potencijal, pa se stoga mogu koristiti kao prirodan izvor biološki aktivnih tvari u prehrani ljudi i ishrani životinja

ENVIRONMENTAL IMPACT ON OIL CONTENT AND FATTY ACID COMPOSITION OF NEW OS-HYBRID COMBINATIONS OF SUNFLOWER

U radu su prikazani rezultati istraživanja 17 novih OS-hibridnih kombinacija suncokreta kroz tri godine (2009.-2011.) na dvije lokacije (Nova Gradiška i Osijek), s ciljem da se utvrdi varijabilnost sadržaja ulja i sastav masnih kiselina u ulju u različitim okolinama. Utvrđene su statistički značajne razlike u sadržaju ulja i sastavu masnih kiselina između godina, lokacija i hibrida. Prosječan sadržaj ulja iznosio je 48,74 %, sadržaj palmitinske kiseline (C16:0) 5,626%, stearinske (C18:0) 3,682%, oleinske (C18:1) 24,741% i linolne (C18:2) 64,447%. Na obje lokacije, najveći je sadržaj ulja bio 2011. godine (50,97%), 2009. oleinske kiseline (27,379%), a 2010. najveći sadržaj linolne kiseline (66,153%). Statistički značajno veći sadržaj ulja, palmitinske i linolne kiseline ostvaren je na lokaciji Nova Gradiška. Na lokaciji Osijek ostvaren je statistički značajno veći sadržaj oleinske kiseline (25,820%). Palmitinska, stearinska i linolna kiselina bile su u statistički visoko značajnoj srednje jakoj do jakoj pozitivnoj korelaciji sa sadržajem ulja, a oleinska kiselina u jakoj negativnoj korelaciji (-0,660**). Odnos oleinske i linolne kiseline bio je statistički značajno u visoko negativnoj korelaciji (-0,974**).This paper presents the results of 17 new OS-hybrid combinations of sunflower during three years (2009-2011) on two locations (Nova Gradiška and Osijek) aiming to determine the variability of oil content and fatty acid composition of the oil in different environments. There were statistically significant differences in oil content and fatty acid composition between years, locations and hybrids. The average oil content was 48.74%, the content of palmitic acid (C16:0) 5.626%, stearic (C18:0) 3.682%, oleic (C18:1), 24.741% and linoleic (C18:2) 64.447%. On both locations, the highest oil content was in 2011 (50.97%), oleic acid (27.379%) in 2009, and in 2010 the highest content of linoleic acid (66.153%). Statistically significant higher oil content, palmitic and linoleic acids were achieved on Nova Gradiška location. Statistically significant higher content of oleic acid (25.820%) was achieved on Osijek location. Palmitic, stearic, and linoleic acids were in statistically high significant medium strong to strong positive correlation with oil content, and oleic acid in a strong negative correlation (-0.660**). The ratio of oleic and linoleic acid was statistically significant in high negative correlation (-0.974**)

KARAKTERIZACIJA SADRŽAJA IZOFLAVONA U HRVATSKOJ KOLEKCIJI CRVENE DJETELINE

Red clover is a perennial forage crop rich in isoflavones, the bioactive compounds with a positive effect on human and animal health. The aim of the research was to determine a variation in the isoflavone contents in the Croatian red clover collection and to identify the most promising materials to be used in the breeding program for the development of new cultivars for specific purposes. Leaf samples of 29 red clover cultivars/populations (two cultivars, twenty breeding populations, and seven local populations) were collected in a full flowering stage, and the identification and quantification of isoflavones was performed using the HPLC analysis. The most common isoflavones in the red clover cultivars/populations were formononetin and biochanin A. A significant variation among the cultivars/populations in the total and individual isoflavone content was determined. The populations with very high and low contents of both the total and of the individual isoflavones were identified to be used for the breeding purposes in order to develop new forage cultivars, or for specific goals in the pharmaceutical industry.Crvena djetelina višegodišnja je krmna kultura bogata izoflavonima, bioaktivnim spojevima s pozitivnim utjecajem na zdravlje ljudi i životinja. Cilj istraživanja bio je utvrditi varijabilnost sadržaja izoflavona hrvatske kolekcije crvene djeteline te identificirati najzanimljivije materijale koji će se koristiti u oplemenjivačkome programu za razvoj novih sorata za specifične namjene. Uzorci lista prikupljeni su u stadiju pune cvatnje iz 29 sorata/populacija crvene djeteline (dvije sorte, dvadeset oplemenjivačkih populacija, sedam lokalnih populacija), a identifikacija i kvantifikacija izoflavona obavljena je pomoću HPLC analize. Najzastupljeniji izoflavoni u sortama/populacijama crvene djeteline bili su formononetin i biohanin A. Utvrđena je značajna varijabilnost među sortama/populacijama u sadržaju ukupnih i individualnih izoflavona. Identificirane su populacije s vrlo visokim i niskim sadržajem kako ukupnih, tako i pojedinačnih izoflavona koje će se koristiti u oplemenjivačke svrhe za razvoj novih krmnih kultivara ili za specifične namjene u farmaceutskoj industrij

Influence of side branches nipping in sunflower restorer lines on seed yield and quality

Tijekom 2005. godine, na pokusnom polju i u laboratoriju Poljopri-vrednog instituta Osijek, obavljena su istraživanja utjecaja zakidanja bočnih grana dviju granatih restorer (RF) linija suncokreta u slobodnoj oplodnji na urod i kakvoću sjemena. Nakon žetve, na osnovu promjera glava, formirane su grupe. Unutar svake grupe određena je masa sjemena, a zatim je sjeme pomoću sita pravokutnih otvora širine od 2 do 5 mm podjeljeno u 4-6 frakcija. Na tri najzastupljenije frakcije, unutar svih grupa sjemena, određena je masa 1000 zrna, energija klijanja i klijavost. Za obe granate linije, promjer centralnih glava je bio od 6 do 14 cm, sa prosječnom masom sjemena po glavi za RF-1 liniju 2.81-11.51 g, a za RF-2 liniju 5.32-13.58 g. Kod linija sa zakinutim bočnim granama, promjeri glava bili su od 8 do 22 cm. Prosječna masa sjemena po glavi za RF-1 liniju bila je 7.55-37.90 g, a za RF-2 liniju 11.55-33.94 g. Masa 1000 zrna se povećavala s povećanjem promjera glave, odnosno frakcije sjemena. Granata RF-1 linija je imala masu 1000 zrna 22.7-40.7 g, odnosno 26.3-73.1 g kod biljaka sa zakinutim bočnim granama. RF-2 linija je imala veću masu 1000 zrna od prethodne linije, kod granatih biljaka bila je 30.0-52.0 g, a kod biljaka sa zakinutim bočnim granama 37.6-91.9 g. Za energiju klijanja i klijavost sjemena, obzirom na promjer glave, utvrđene su statistički značajne razlike (P=0.01) za obe granate linije, te za RF-2 liniju sa zakinutim bočnim granama. Obzirom na frakcije sjemena, statistički značajne razlike (P=0.05) nađene su za klijavost sjemena na RF-1 liniji sa zakinutim bočnim granama, te na granatoj RF-2 liniji, dok za energiju klijanja nije utvrđena značajna razlika za analizirane grupe.During 2005 year, on the trial field and laboratory of the Agricultural Institute Osijek have done researches of side branches nipping influence in two sunflower branched restorer (RF) lines in open pollination on seed yield and quality. After harvesting, on the basis of head diameter, groups were formed. Within each group seed mass is determined, followed by seed separation in 4-6 fractions using sieves of oblong holes width from 2 to 5 mm. On the largest three fractions, within all seed groups, were determined mass of 1000 grains, vigor and germination. For both branched lines, diameter of central heads was from 6 to 14 cm, with average seed mass per head in RF-1 line 2.81-11.51 g, and in RF-2 line 5.32-13.58 g. In lines with nipped side branches, diameters of heads were from 8 to 22 cm. Average seed mass per head in RF-1 line was 7.55-37.90 g, and in RF-2 line 11.55-33.94 g. Mass of 1000 grains has increased with increasing of head diameter and seed fraction, respectively. Branched RF-1 line had mass of 1000 grains 22.7-40.7 g, and 26.3-73.1 g in plants with nipped side branches. RF-2 line had higher mass of 1000 grains in relation to the previous line, in branched plants 30.0-52.0 g, and in nipped side branches plants 37.6-91.9 g. For vigor and seed germination, regarding head diameter, have established statistically significant differences (P=0.01) in both branched lines, and RF-2 line with nipped side branches. Regarding seed fractions, statistically significant differences (P=0.05) have found for seed germination in RF-1 line with nipped side branches, and branched RF-2 line, while for vigor have not been significant differences in all analyzed groups

EFFICIENCY OF FINISHING MACHINES IN THE BUSINESS PROCESS OF ALFALFA SEED PRODUCTION

U procesu proizvodnje kvalitetnog sjemena lucerne potrebno je proizvesti sjeme te ga adekvatno doraditi i skladištiti u skladu sa zakonskim aktima i pravilima struke. Stoga je i cilj ovog istraživanja bio utvrditi koji su dijelovi dorade kritične točke dorade te odrediti stupnjeve iskoristivosti svake pojedine faze dorade i tako utvrditi koja faza dorade koliko doprinosi kvaliteti sjemena. U RH najveće količine sjemena proizvode se na području Istarske županije i provedenim istraživanjem je obuhvaćeno pet proizvođača s navedenog agroekološkog područja tijekom tri uzastopne proizvodne godine (2017., 2018. i 2019.). Primarna dorada odrađena je na selektoru i gravitacijskom odjeljivaču, a sekundarna na magnetnom odjeljivaču. Čistoća naturalnog sjemena dopremljenog na doradu kretala seu rasponu od 76% do 92%. Iskoristivost sjemena u doradi u trogodišnjem istraživanju iznosio je 76,14 %. U procesu dorade sjemena lucerne najveća količina otpada bila je na selektoru i kretala se od 16,6% do 19,8%, dok su otpadi na magnetnom odjeljivaču u svim godinama dorade bili sličnih vrijednosti i iznosili u prosjeku 5,9 %. Proces dorade potrebno je provoditi sve dok se ne dostigne propisana kvaliteta sjemena. Rezultati istraživanja o učinkovitosti strojeva za doradu sjemena lucerne ukazuju na ovisnost o ulaznim parametrima naturalnog sjemena, a najviše o prisustvu brojnih primjesa na konačnu količinu i kvalitetu sjemena lucerne.Alfalfa is a perennial plant that is considered the most important foddercrop for the production of quality fodder. Ensuring sufficient quantities of quality seeds is the first prerequisite in the process of producing quality foddermass of alfalfa, and high-value seeds are obtained by processing and properstorage of processed seeds. The aim of the research was to determine the yields of processing after each phase of processing in the process of alfalfa seed processing and thus determine contribution of each phase of processing to the quality of seeds. In the Republic of Croatia, the largest quantities of alfalfa seeds are produced in the Istrian County, and the conducted research covered five producers from the mentioned agro-ecological are a during three consecutive production years (2017, 2018 and 2019). The primary processing was done on a selector and a gravity separator, and the secondary on a magnetic separator. The purity of natural seeds delivered for processing ranged from 76% to 92%. The processing yield in the three-year survey was 76.14%. In processing alfalfa seeds, the largest amount of waste was on the selector and ranged from 16.6% to 19.8%, while the waste on the magnetic separator in all years of processing was of similar value and averaged 5.9%. Seed processing must be carried out until the prescribed seed quality is reached. The results of research on the efficiency of alfalfa seed processing machines indicate the dependence on the input parameters of natural seeds, and most of all on the presence of numerous impurities on the final quantity and quality of alfalfa seeds

Combining abilities and gene effect for 1000 seed weight and hectoliter mass in sunflower ( Helianthus annuus L.)

Masa 1000 zrna i hektolitarska masa su svojstva kojima se u oplemenjivačkom programu na suncokretu u Poljoprivrednom institutu Osijek poklanja značajna pozornost. Važan segment kod izbora po-željnih linija za navedena svojstva predstavlja procjena općih (OKS) i specifičnih (SKS) kombinacijskih sposobnosti, učinaka gena te izdvajanje najboljih hibridnih kombinacija. Istraživanjem je obuhvaćeno 6 inbred linija, 15 dvolinijskih križanaca, 15 eksperimentalnih trolinijskih križanaca i tri priznata hibrida. U procjeni kombinacijskih sposobnosti korištena je Griffingova analiza dialelnih križanja. Najvećom masom 1000 zrna u prvoj godini istraživanja (1999) ističe se 5A x 302 B, a u drugoj godini (101A x 103B) x RH- 28 i Fakir. Najviše vrijednosti hektolitarske mase u prvoj godini imali su križanci 5A x 103B i 5A x 302B, a u drugoj (272A x 302B) x RH- 28 i Olio. Linija L-5 ostvarila je najveće učinke OKS za oba istraživana svojstva, a pored nje dobar opći kombinator za masu 1000 zrna je linija L-103. Najbolje kombi-nacije za masu 1000 zrna su 5A x 302B i 101A x 103B. Učinci SKS za hektolitarsku masu nisu bili značajni. Veći je udio genetske u odnosu na okolinsku, te aditivne u odnosu na dominantnu varijancu za masu 1000 zrna.Thousand seed weight and hectoliter mass are traits which in sunflower breeding program at Agricultural Institute Osijek is given significant attention. Important part in selection of desirable lines for mentioned traits presents estimation of general (GCA) and specific (SCA) combining ability, gene effect and selection of the best hybrid combinations. This investigation conducts 6 inbread lines, 15 two-way crosses, 15 experimental three-way crosses and three recognized hybrids. Griffings\u27 analysis of diallel crossing is used in combining ability estimation. The highest 1000 seed weight in first year of investigation (1999) had combination 5A x 302 B, and in second year (101A x 103B) x RH- 28 and Fakir. The highest hectoliter mass values in first year had crosses 5A x 103B and 5A x 302B, and in second (272A x 302B) x RH- 28 and Olio. Line L-5 obtained the highest effects of GCA for both investigated traits, and beside this line, good general combinatory for 1000 seed weight is line L-103. The best combinations for 1000 seed weight are 5A x 302B and 101A x 103B. An effect of SCA for hectoliter mass wasn’t significant. Higher is a part of genetic in relation to environmental, and additive in relation to dominant variance for 1000 seed weight

EFFECT OF ENVIRONMENT ON QUANTITATIVE TRAITS OF NEW OS SUNFLOWER HYBRIDS

Gospodarsku vrijednost nekog genotipa (hibrida ili sorte) ne čini samo njegova produktivnost, otpornost na bolesti, sušu, polijeganje itd., već sposobnost da pozitivna svojstva zadrži u različitim okolinskim uvjetima proizvodnje. U prirodi postoji jaka interakcija genotipa i okoline, koju svaki oplemenjivač nastoji procjeniti nakon stvaranja novih genotipova. U 2008. godini, na polju Poljoprivrednog instituta Osijek, napravljene su 24 nove hibridne kombinacije suncokreta križanjem tri citoplazmatski muško sterilne linije i osam restorer linija. Tijekom 2009. i 2010. godine, postavljeni su poljski pokusi na lokacijama: Beli Manastir (BM09 i BM10), Osijek (Os09 i Os10) i Nova Gradiška (NG09 i NG10), a 2010. pokus je postavljen i u Vukovaru (Vu10). Iz podataka uroda zrna, sadržaja ulja te uroda ulja, za obje godine i sve lokacije (7 okolina), napravljena je analiza varijance (ANOVA) i Additive Main effects and Multiplicative Interactions (AMMI) analiza te AMMI1 i AMMI2 biploti. Hibridi su se značajno razlikovali u urodu zrna, sadržaju ulja i urodu ulja po istraživanim lokacijama i godinama. Prosječno, za sve istraživane hibride u sedam okolina, urod zrna je bio 3,3 t/ha, sadržaj ulja 47,5 %, a urod ulja 1,423 t/ha. Značajno najveći urod zrna i ulja ostvaren je u okolini Os09 (4,220 i 1,831 t/ha), a sadržaj ulja u NG10 (50,6 %). Najveći urod zrna, u sedam okolina, ostvario je hibrid OS-H-4 (3,913 t/ha). Ovaj hibrid je imao i najveći urod ulja (1,721 t/ha). Najveći sadržaj ulja u zrnu suncokreta imao je hibrid OS-H-1 (50,6 %). AMMI analizom uroda zrna, sadržaja ulja i uroda ulja, prve dvije IPCA komponente bile su značajne na razini 0,01, odnosno 0,05, i objašnjavaju 58,5, 73,7 i 60,5 % od ukupne interakcije genotip x okolina. AMMI1 i AMMI2 biploti su pokazali da je hibrid s najvećim urodom zrna i ulja (OS-H-4), ispoljio malu interakciju sa okolinom, odnosno imao je veliku stabilnost, a hibrid koji je imao najveći sadržaj ulja (OS-H-1) imao je veliku interakciju s okolinom, odnosno malu stabilnost. Pored toga, hibridi OS-H-12, OS-H-15 i OS-H-19 ispoljili su dobru stabilnost za urod zrna, OS-H-4, OS-H-7 i OS-H-18 za sadržaj ulja, a OS-H-1, OS-H-9 i OS-H-18 z a urod u lja. O vi h ibridi s u i mali z a s va t ri istraživana svojstva vrijednosti značajno bolje od prosjeka svih okolina.The economic value of a certain genotype (hybrid or variety) is not only his productivity, resistance to diseases, drought, lodging, etc., but the ability to maintain positive properties in different environmental conditions of production. In nature there is a strong interaction between genotype and environment, which every breeder tries to evaluate after the creation of new genotypes. In 2008, in the field of Agricultural Institute Osijek were made 24 new sunflower hybrid combinations by crossing three cytoplasmic male sterile lines and eight restorer lines. During 2009 and 2010, field trials were set up at locations: Beli Manastir (BM09 and BM10), Osijek (Os09 and Os10), Nova Gradiška (NG09 and NG10), and in 2010 the field trial was set up in Vukovar (Vu10). On the data of grain yield, oil content and oil yield, for both years and all locations (7 environments), is performed analysis of variance (ANOVA) and Additive Main effects and Multiplicative Interactions (AMMI) analysis by the statistical program SAS for Windows 9.1. Also, for analyzed traits are made AMMI1 and AMMI2 biplots by the program IRRISTAT for Windows 5.0. Grain yield, oil content and oil yield were significantly different at the tested locations and years. On average, for all investigated hybrids in seven environments, grain yield was 3.300 t/ha, oil content 47.48 % and oil yield 1.423 t/ha. Significantly the highest grain and oil yield was achieved in an environment Os09 (4.220 and 1.831 t/ha) and oil content in NG10 (50.59 %). The highest grain yield, in seven environments, had a hybrid OS-H-4 (3.913 t/ha). This hybrid had also the highest oil yield (1.721 t/ha). The highest oil content in sunflower grain had a hybrid OS-H-1 (50.58 %). By AMMI analysis of grain yield, oil content and oil yield, the first two IPCA components were significant at the 0.01 or 0.05, and explained 58.5, 73.7 and 60.5% of the total genotype x environment interaction. AMMI1 and AMMI2 biplots showed that the hybrid with the largest grain and oil yield (OS-H-4), showed small interaction with the environment, and had a great stability, and hybrid that had the highest oil content (OS-H-1) had a great interaction with the environment, and small stability. In addition, hybrid OS-H-12, 15 and OS-H 19 have displayed a good stability for grain yield, OS-H-4, 7 and OS-H 18 for oil content, and OS-H-1, OS-H 9 and O S-H 18 for oil yield. These hybrids had all three tested traits significantly better than the average of 7 environments