Experiences and issues for environmental science sensor network deployments

Sensor network research is a large and growing area of academic effort, examining technological and deployment issues in the area of environmental monitoring. These technologies are used by environmental engineers and scientists to monitor a multiplicity of environments and services, and, specific to this paper, energy and water supplied to the built environment. Although the technology is developed by Computer Science specialists, the use and deployment is traditionally performed by environmental engineers. This paper examines deployment from the perspectives of environmental engineers and scientists and asks what computer scientists can do to improve the process. The paper uses a case study to demonstrate the agile operation of WSNs within the Cloud Computing infrastructure, and thus the demand-driven, collaboration-intense paradigm of Digital Ecosystems in Complex Environments

Experiences and issues for environmental engineering sensor network deployments

Sensor network research is a large and growing area of academic effort, examining technological and deployment issues in the area of environmental monitoring. These technologies are used by environmental engineers and scientists to monitor a multiplicity of environments and services, and, specific to this paper, energy and water supplied to the built environment. Although the technology is developed by Computer Science specialists, the use and deployment is traditionally performed by environmental engineers. This paper examines deployment from the perspectives of environmental engineers and scientists and asks what computer scientists can do to improve the process. The paper uses a case study to demonstrate the agile operation of WSNs within the Cloud Computing infrastructure, and thus the demand-driven, collaboration-intense paradigm of Digital Ecosystems in Complex Environments

Halo naevi, vitiligo and diffuse alopecia areata associated with tocilizumab therapy

We present a follow-up case report of a 33-year-old lady with juvenile onset arthritis who developed halo naevi while on treatment with tocilizumab. This case report describes the development of halo naevi, vitiligo and diffuse alopecia areata associated with tocilizumab therapy following infection with Methicillin-resistant Staphylococcus aureus (MRSA) and Panton-Valentine leukocidin positivity. This is the first case that describes these events and supports previous theories on cellular and humoral immunity as causative factors. The regression of melanocytes during treatment with tocilizumab could also implicate IL-6 and sIL-6R as future targets in the treatment of melanoma through its direct effect of melanocytic cytotoxicity, which supports previous studies

Plucked hair follicles from patients with chronic discoid lupus erythematosus show a disease-specific molecular signature

Objective: When faced with clinical symptoms of scarring alopecia—the standard diagnostic pathway involves a scalp biopsy which is an invasive and expensive procedure. This project aimed to assess if plucked hair follicles (HFs) containing living epithelial cells can offer a non-invasive approach to diagnosing inflammatory scalp lesions. Methods: Lesional and non-lesional HFs were extracted from the scalp of patients with chronic discoid lupus erythematosus (CDLE), psoriasis and healthy controls. RNA was isolated from plucked anagen HFs and microarray, as well as quantitative real-time PCR was performed. Results: Here, we report that gene expression analysis of only a small number of HF plucked from lesional areas of the scalp is sufficient to differentiate CDLE from psoriasis lesions or healthy HF. The expression profile from CDLE HFs coincides with published profiles of CDLE from skin biopsy. Genes that were highly expressed in lesional CDLE corresponded to well-known histopathological diagnostic features of CDLE and included those related to apoptotic cell death, the interferon signature, complement components and CD8+ T-cell immune responses. Conclusions: We therefore propose that information obtained from this non-invasive approach are sufficient to diagnose scalp lupus erythematosus. Once validated in routine clinical settings and compared with other scarring alopecias, this rapid and non-invasive approach will have great potential for paving the way for future diagnosis of inflammatory scalp lesions

Qualitative interpretation and sampling validity of peer recognition study

In an examination of limitations to a previous study, it was found that there was no significant difference between respondents and non-respondents with regard to country or eminence of institution. Observations from non-respondents illustrate possible constraints on the interpretation of responses.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/23847/1/0000086.pd

Type six secretion system of Bordetella bronchiseptica and adaptive immune components limit intracellular survival during infection

The Type Six Secretion System (T6SS) is required for Bordetella bronchiseptica cytotoxicity, cytokine modulation, infection, and persistence. However, one-third of recently sequenced Bordetella bronchiseptica strains of the predominantly human-associated Complex IV have lost their T6SS through gene deletion or degradation. Since most human B. bronchiseptica infections occur in immunocompromised patients, we determine here whether loss of Type Six Secretion is beneficial to B. bronchiseptica during infection of immunocompromised mice. Infection of mice lacking adaptive immunity (Rag1-/- mice) with a T6SS-deficient mutant results in a hypervirulent phenotype that is characterized by high numbers of intracellular bacteria in systemic organs. In contrast, wild-type B. bronchiseptica kill their eukaryotic cellular hosts via a T6SS-dependent mechanism that prevents survival in systemic organs. High numbers of intracellular bacteria recovered from immunodeficient mice but only low numbers from wild-type mice demonstrates that B. bronchiseptica survival in an intracellular niche is limited by B and T cell responses. Understanding the nature of intracellular survival during infection, and its effects on the generation and function of the host immune response, are important to contain and control the spread of Bordetella-caused disease.Liron Bendor, Laura S. Weyrich, Bodo Linz, Olivier Y. Rolin, Dawn L. Taylor, Laura L. Goodfield, William E. Smallridge, Mary J. Kennett, Eric T. Harvil