Met signaling in cardiomyocytes is required for normal cardiac function in adult mice

et al.Hepatocyte growth factor (HGF) and its receptor, Met, are key determinants of distinct developmental processes. Although HGF exerts cardio-protective effects in a number of cardiac pathologies, it remains unknown whether HGF/Met signaling is essential for myocardial development and/or physiological function in adulthood. We therefore investigated the requirement of HGF/Met signaling in cardiomyocyte for embryonic and postnatal heart development and function by conditional inactivation of the Met receptor in cardiomyocytes using the Cre-α-MHC mouse line (referred to as α-MHCMet-KO). Although α-MHCMet-KO mice showed normal heart development and were viable and fertile, by 6. months of age, males developed cardiomyocyte hypertrophy, associated with interstitial fibrosis. A significant upregulation in markers of myocardial damage, such as β-MHC and ANF, was also observed. By the age of 9. months, α-MHCMet-KO males displayed systolic cardiac dysfunction. Mechanistically, we provide evidence of a severe imbalance in the antioxidant defenses in α-MHCMet-KO hearts involving a reduced expression and activity of catalase and superoxide dismutase, with consequent reactive oxygen species accumulation. Similar anomalies were observed in females, although with a slower kinetics. We also found that Met signaling down-regulation leads to an increase in TGF-β production and a decrease in p38MAPK activation, which may contribute to phenotypic alterations displayed in α-MHCMet-KO mice. Consistently, we show that HGF acts through p38α to upregulate antioxidant enzymes in cardiomyocytes. Our results highlight that HGF/Met signaling in cardiomyocytes plays a physiological cardio-protective role in adult mice by acting as an endogenous regulator of heart function through oxidative stress control.This work was supported by grants: (AFM)-13683 from Association Française contre les myopathies, France, FIS-PI07/0071 and SAF-2010-20198-C02-01 from Ministry of Science and Innovation, Spain, and grants from Comunidad de Madrid/Universidad Complutense de Madrid: CAM/UCM 920384 and UCM-BSCH 920384, Spain to A.P.; BFU2011-25304 from Ministry of Science and Innovation, Spain, RD12/0019/0022 (TerCel network, ISCIII), P11-CTS-7564 (Junta de Andalucía) to R. M.-Ch.; FRM (Fondation pour la Recherche Médicale), Fondation Bettencourt-Schueller, and Association Française contre les myopathies (AFM) to F.M.; SAF2010-15881 from Ministry of Science and Innovation, Spain, and RD012/0021 (RedinRen network, ISCIII), and GR100 (Junta de Castilla y León) to J.M. L.-N. The cardiovascular phenotyping unit of the University of Salamanca, including the telemetry equipment, has been acquired with the support of the European Regional Development Funds (FEDER).Peer Reviewe

Remediation of metal-contaminated soils with the addition of materials - Part II: Leaching tests to evaluate the efficiency of materials in the remediation of contaminated soils

The effect of the addition of materials on the leaching pattern of As and metals (Cu, Zn, Ni, Pb, and Cd) in two contaminated soils was investigated. The examined materials included bentonites, silicates and industrial wastes, such as sugar foam, fly ashes and a material originated from the zeolitization of fly ash. Soil+material mixtures were prepared at 10% doses. Changes in the acid neutralization capacity, crystalline phases and contaminant leaching over a wide range of pHs were examined by using pH stat leaching tests. Sugar foam, the zeolitic material and MX-80 bentonite produced the greatest decrease in the leaching of pollutants due to an increase in the pH and/or the sorption capacity in the resulting mixture. This finding suggests that soil remediation may be a feasible option for the reuse of non-hazardous wastes.Ministerio de Educación y Ciencia CTQ2010-14874, CTM2008-01147Ministerio de Medio Ambiente A536/2007/3-01.2, 300/PC/08/3-01.

Ultrasound Strain Elastography Reliability in the Assessment of the Plantar Fascia and Its Relationship with the Plantar Thickness in Healthy Adults: An Intra and Interobserver Reliability Study in Novice Evaluators

Partial funding for open access charge: Universidad de Málag

Experimental adiabatic vortex ratchet effect in Nb films with asymmetric pinning trap

Nb films grown on top of an array of asymmetric pinning centers show a vortex ratchet effect. A net flow of vortices is induced when the vortex lattice is driven by fluctuating forces on an array of pinning centers without reflection symmetry. This effect occurs in the adiabatic regime and it could be mimiced only by reversible DC driven forces

Experimental vortex ratchet effect in nanostructured superconductors

Superconducting Nb thin films were grown on different arrays of triangle-shape metallic islands. The vortex lattice dynamics could be strongly modified by these asymmetric vortex traps. These asymmetric pinning potentials lead to a rectification effect on the vortex motion: Injecting an ac supercurrent on the sample yields a net dc vortex flow. This vortex ratchet ffect is adiabatic and reversible: The effect is frequency independent and the polarity of the dc voltage output could be tuned by the applied magnetic fields and the input ac currents

Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation

Fridericia caudigera and Cuspidaria convoluta (Bignoniaceae) species, which grow in the northwest of Argentina, have shown antibacterial effect against strains isolated from skin infections, and each one displayed synergism with commercial antibiotics. The aims of this work were to evaluate the antibacterial activity and toxicity of the combination of these two plant species, and to design a stable gel for topical use including the blend of extracts. The combination of extracts was evaluated for synergistic effects (chequerboard assay), genotoxicity (Ames test) and cytotoxicity (Artemia salina test). A gel was subsequently formulated with the combination of extracts using carboxymethylcellulose as a polymer. The following physicochemical characteristics of the gel formulation: pH, viscosity, spreadability and total phenol content, as well as resistance to severe temperature changes, biological activity (diffusion in agar), in vitro permeation (Franz cells) and primary dermal irritation (Draize test) were analyzed. The combination of extracts showed a synergistic effect on pathogenic bacteria and was not toxic in the in vitro tests. The gel was stable and retained the antimicrobial activity of the original extracts. The formulation proposed in this work could constitute an alternative for primary skin infections since it proved to be safe for topical administration.Fil: Torres, Carola Analía. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Nordeste. Instituto de Investigaciones En Procesos Tecnologicos Avanzados. - Universidad Nacional del Chaco Austral. Instituto de Investigaciones En Procesos Tecnologicos Avanzados.; ArgentinaFil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Nordeste. Instituto de Investigaciones En Procesos Tecnologicos Avanzados. - Universidad Nacional del Chaco Austral. Instituto de Investigaciones En Procesos Tecnologicos Avanzados.; ArgentinaFil: Nuñez, María Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Nordeste. Instituto de Investigaciones En Procesos Tecnologicos Avanzados. - Universidad Nacional del Chaco Austral. Instituto de Investigaciones En Procesos Tecnologicos Avanzados.; ArgentinaFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentin

Short-term effect of eucalyptus leachates on green food webs in headwaters

Despite the reported allelopathic nature of the Eucalyptus genus, eucalypt leachates have unknown effects on the trophic base of stream green food webs. Eucalypt plantations have increased worldwide, including riparian ecosystems. We aimed to test whether short-term eucalypt leachates might alter water chemistry, periphytic algal biomass and diatoms, and herbivorous invertebrates’ (i.e., scrapers). We studied two oligotrophic and well-preserved headwaters from NW Spain. The experiment followed a before-after control-impact paired (BACIp) design, with weekly sampling before and after eucalypt leaves addition to streams. In the stream with lower discharge, the eucalypt treatments seemed to affect increases in biomass accrual (Chl-a) and diatom assemblages, disfavouring sensitive species (e.g., Eunotia minor and Achnanthidium pyrenaicum). Therefore, the ecological status was reduced from high to good. In the stream with higher discharge, invertebrate assemblages changed with scrapers having their abundances modified in comparison with the control (e.g., Habrophlebia sp. and Elmidae). Results suggest that eucalypt leaves leachates exert toxic effects on periphyton and on diatom and invertebrate assemblages, and this effect might be mediated by discharge. Alterations at the base of stream food webs may compromise their good ecological status. Further studies are necessary to identify whether this toxicity is due to allelopathic processes

Actividad antilipooxigenasa de extractos de rhipsalis lumbricoides

Introducción. Las plantas del género Rhipsalis son cactus epífitos que crecen sobre troncos y ramas de los árboles, caracterizados por sus delgados tallos verdes péndulos. El nombre vulgar de Rhipsalis lumbricoides es suelda consuelda y se emplea tradicionalmente por sus efectos anti-inflamatorios, en esguinces, fisuras y fracturas.Objetivo. Evaluar el potencial efecto antiinflamatorio de distintos extractos de R. lumbricoides mediante un método in vitro.Experimental. El material vegetal fue colectado en Villa Río Bermejito-Chaco, secado al sol, molido y llevado a un tamaño de polvo uniforme. Posteriormente se obtuvieron los extractos usando dos métodos: maceración en frío y Soxhlet. Se utilizaron tres solventes de diferentes polaridades, éter de petróleo, acetato de etilo y etanol. Cada uno de los extractos fue concentrado en rotavapor, llevado a sequedad y posteriormente redisuelto en alcohol 96° a una concentración de 10 mg/ml.La actividad antiinflamatoria in vitro se evaluó mediante el efecto inhibitorio sobre la actividad de la lipooxigenasa siguiendo la metodología descrita por Taraporewala & Kauffman (1990). Se midió la cantidad de hidroperóxidos lipídicos producidos a partir del ácido linoleico a 234 nm. La mezcla de reacción contenía 5 mM de ácido linoleico en buffer borato 0,2 M - pH 9 y 0,9 nM de 1-lipoxigenasa de soja en buffer pH 9 y diferentes concentraciones del extracto seco redisuelto en etanol. Se usó indometacina como control positivo. Los resultados se expresaron como valores de IC50 expresados en µg de extracto seco usados/ml de reacción.Resultados y Discusión. Los dos extractos etanólicos y el de acetato de etilo demostraron tener efecto inhibitorio frente a la enzima lipooxigenasa. El extracto de acetato de etilo y el macerado etanólico, mostraron una mayor inhibición, con valores de IC50 de 39,23 para el de acetato de etilo y 60,19 µg/mL en el macerado etanólico. Conclusiones. Estos resultados aportan información respecto del uso popular de R. lumbricoides mediante un modelo in vitro. Se necesitan pruebas adicionales y ensayos in vivo para continuar este estudio.Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Saravia, Jimena Agostina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaI Encuentro Iberoamericano de Investigación en Ciencias Farmacéuticas y XIV Jornadas Día del FarmacéuticoComodoro RivadaviaArgentinaUniversidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales y Ciencias de la Salu

Typification of names in Manettia (Rubiaceae, Spermacoceae) for the Flora of Argentina and Paraguay

Manettia is a Neotropical genus that includes 80 to 130 species. As part of the taxonomical review of the genus for the “Flora Argentina” and “Flora del Paraguay” projects, seven names were found in need of nomenclatural studies. As a result five lectotypes and two neotypes are here designated and notes on these typifications are included.Manettia es un género neotropical que incluye unas 80 a 130 especies. Como parte de la revisión taxonómica del género para los proyectos de “Flora Argentina” y “Flora del Paraguay” se hallaron siete nombres que necesitan estudios nomenclaturales. Como resultado se designan cinco lectotipos y dos neotipos y se incluyen notas sobre las tipificaciones.Fil: Gauto, Silvana Yasmin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Nuñez Florentin, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Salas, Roberto Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentin

Aprendizaje asistido por dispositivos móviles: UP2B2

Este trabajo se enmarca en el enfoque denominado Mobile Assisted Language Learning (MALL) y presenta un prototipo de juego de preguntas y respuestas de opción múltiple que revisa la gramática, el vocabulario y el uso de la lengua inglesa del nivel B2 (Marco Común Europeo de Referencia para las Lenguas-MCERL). La aplicación se caracteriza por presentar una componente de gamificación cuyo objetivo es motivar a los estudiantes a que la usen mediante un sistema de puntuación que los sitúa en un ranking global y que considera tanto los aciertos de cada uno como el tiempo en completar los tests. Se definen distintos índices para la monitorización de la plataforma e indicadores tanto de carácter cualitativo como cuantitativo para la medida de los resultados. ABSTRACT This work is framed within the approach called Mobile Assisted Language Learning (MALL) and presents a game prototype consisting of multiple choice questions and answers that assess the grammar, vocabulary and the use of the English language at level B2 according to the Common European Framework of Reference for Languages. The application is characterized by a strong gamification component that aims to motivate the students using it. Therefore, response time, correct answers and a ranking with the punctuation centre the most prominent aspects related to its playability. Different indexes are defined for the monitoring of the platform as well as qualitative and quantitative indicators for the study of the results