HADES RV Programme with HARPS-N at TNG. VII. Rotation and activity of M-Dwarfs from time-series high-resolution spectroscopy of chromospheric indicators

We aim to investigate the presence of signatures of magnetic cycles and rotation on a sample of 71 early M-dwarfs from the HADES RV programme using high-resolution time-series spectroscopy of the Ca II H & K and Halpha chromospheric activity indicators, the radial velocity series, the parameters of the cross correlation function and the V-band photometry. We used mainly HARPS-N spectra, acquired over four years, and add HARPS spectra from the public ESO database and ASAS photometry light-curves as support data, extending the baseline of the observations of some stars up to 12 years. We provide log(R'hk) measurements for all the stars in the sample, cycle length measurements for 13 stars, rotation periods for 33 stars and we are able to measure the semi-amplitude of the radial velocity signal induced by rotation in 16 stars. We complement our work with previous results and confirm and refine the previously reported relationships between the mean level of chromospheric emission, measured by the log(R'hk), with the rotation period, and with the measured semi-amplitude of the activity induced radial velocity signal for early M-dwarfs. We searched for a possible relation between the measured rotation periods and the lengths of the magnetic cycle, finding a weak correlation between both quantities. Using previous v sin i measurements we estimated the inclinations of the star's poles to the line of sight for all the stars in the sample, and estimate the range of masses of the planets GJ 3998 b and c (2.5 - 4.9 Mearth and 6.3 - 12.5 Mearth), GJ 625 b (2.82 Mearth), GJ 3942 b (7.1 - 10.0 Mearth) and GJ 15A b (3.1 - 3.3 Mearth), assuming their orbits are coplanar with the stellar rotation.Comment: 19 pages, 16 figures, 10 table

The HADES RV Programme with HARPS-N at TNG XI. GJ 685 b: a warm super-Earth around an active M dwarf

Small rocky planets seem to be very abundant around low-mass M-type stars. Their actual planetary population is however not yet precisely understood. Currently several surveys aim to expand the statistics with intensive detection campaigns, both photometric and spectroscopic. We analyse 106 spectroscopic HARPS-N observations of the active M0-type star GJ 685 taken over the past five years. We combine these data with photometric measurements from different observatories to accurately model the stellar rotation and disentangle its signals from genuine Doppler planetary signals in the RV data. We run an MCMC analysis on the RV and activity indexes time series to model the planetary and stellar signals present in the data, applying Gaussian Process regression technique to deal with the stellar activity signals. We identify three periodic signals in the RV time series, with periods of 9, 24, and 18 d. Combining the analyses of the photometry of the star with the activity indexes derived from the HARPS-N spectra, we identify the 18 d and 9 d signals as activity-related, corresponding to the stellar rotation period and its first harmonic respectively. The 24 d signals shows no relations with any activity proxy, so we identify it as a genuine planetary signal. We find the best-fit model describing the Doppler signal of the newly-found planet, GJ 685\,b, corresponding to an orbital period $P_b = 24.160^{+0.061}_{-0.047}$ d and a minimum mass $M_P \sin i = 9.0^{+1.7}_{-1.8}$ M$_\oplus$. We also study a sample of 70 RV-detected M-dwarf planets, and present new statistical evidence of a difference in mass distribution between the populations of single- and multi-planet systems, which can shed new light on the formation mechanisms of low-mass planets around late-type stars.Comment: 18 pages, 13 figures, accepted for publication in A&

HADES RV Programme with HARPS-N at TNG VI. GJ 3942 b behind dominant activity signals

Short- to mid-term magnetic phenomena on the stellar surface of M-type stars cannot only resemble the effects of planets in radial velocity data, but also may hide them. We analyze 145 spectroscopic HARPS-N observations of GJ 3942 taken over the past five years and additional photometry to disentangle stellar activity effects from genuine Doppler signals as a result of the orbital motion of the star around the common barycenter with its planet. To achieve this, we use the common methods of pre-whitening, and treat the correlated red noise by a first-order moving average term and by Gaussian-process regression following an MCMC analysis. We identify the rotational period of the star at 16.3 days and discover a new super-Earth, GJ 3942 b, with an orbital period of 6.9 days and a minimum mass of 7.1 Me. An additional signal in the periodogram of the residuals is present but we cannot claim it to be related to a second planet with sufficient significance at this point. If confirmed, such planet candidate would have a minimum mass of 6.3 Me and a period of 10.4 days, which might indicate a 3:2 mean-motion resonance with the inner planet

Prevención del cólera aviar con extractos acuosos de plantas en pollos de engorde.

De octubre a diciembre de 1992, con extractos acuosos de las plantas, se realizaron, anfitoquímicos y pruebas microbiológicas en la bacteria Pasteurella multocida. La etapa de campo se realizó en la Granja Escuela de Capacitación Cooperativa Agropecuaria (GECA) en Chalatenango, El Salvador. Se evaluaron 16 tratamientos: cuatro plantas individuales, sus posibles combinaciones y el testigo. Se sometieron pollos de engorde, de la línea Arbos Acres, a la inoculación de la bacteria, para determinar el resultado preventivo de los tratamientos. Con el modelo de Weibull y la función de supervivencia se obtuvo significancia estadística solamente para los tratamientos con las combinaciones (Quina-Epacina, Palo hediondo-Tempate, Palo hediondo- Epacina y Quina- Tempate - Palo hediondo-Epacina; éste último tratamiento fue el mejor para la prevención del cólera aviar

The HADES RV Programme with HARPS-N@TNG VIII. Gl15A: A multiple wide planetary system sculpted by binary interaction

We present 20 years of radial velocity (RV) measurements of the M1 dwarf Gl15A, combining 5 years of intensive RV monitoring with the HARPS-N spectrograph with 15 years of archival HIRES/Keck RV data. We carry out an MCMC-based analysis of the RV time series, inclusive of Gaussian Process (GP) approach to the description of stellar activity induced RV variations. Our analysis confirms the Keplerian nature and refines the orbital solution for the 11.44-day period super Earth, Gl15A\,b, reducing its amplitude to $1.68^{+0.17}_{-0.18}$ m s$^{-1}$ ($M \sin i = 3.03^{+0.46}_{-0.44}$ M$_\oplus$), and successfully models a long-term trend in the combined RV dataset in terms of a Keplerian orbit with a period around 7600 days and an amplitude of $2.5^{+1.3}_{-1.0}$ m s$^{-1}$, corresponding to a super-Neptune mass ($M \sin i = 36^{+25}_{-18}$ M$_\oplus$) planetary companion. We also discuss the present orbital configuration of Gl15A planetary system in terms of the possible outcomes of Lidov-Kozai interactions with the wide-separation companion Gl15B in a suite of detailed numerical simulations. In order to improve the results of the dynamical analysis, we derive a new orbital solution for the binary system, combining our RV measurements with astrometric data from the WDS catalogue. The eccentric Lidov-Kozai analysis shows the strong influence of Gl15B on the Gl15A planetary system, which can produce orbits compatible with the observed configuration for initial inclinations of the planetary system between $75^\circ$ and $90^\circ$, and can also enhance the eccentricity of the outer planet well above the observed value, even resulting in orbital instability, for inclinations around $0^\circ$ and $15^\circ - 30^\circ$. The Gl15A system is the multi-planet system closest to Earth, at $3.57$ pc, and hosts the longest period RV sub-jovian mass planet discovered so far.Comment: 19 pages, 14 figures, accepted for publication in A\&

HADES RV Programme with HARPS-N at TNG XIII. A sub-Neptune around the M dwarf GJ 720 A

Context. The high number of super-Earth and Earth-like planets in the habitable zone (HZ) detected around M-dwarf stars in the last years has revealed these stellar objects to be the key for planetary radial velocity (RV) searches. Aims. Using the HARPS-N spectrograph within The HArps-n red Dwarf Exoplanet Survey (HADES) we reach the precision needed to detect small planets with a few Earth masses using the RV technique. Methods. We obtained 138 HARPS-N RV measurements between 2013 May and 2020 September of GJ 720 A, classified as an M0.5V star located at a distance of 15.56 pc. To characterize the stellar variability and to discern the periodic variation due to the Keplerian signals from those related to stellar activity, the HARPS-N spectroscopic activity indicators and the simultaneous photometric observations were analyzed. The combined analysis of HARPS-N RVs and activity indicators let us to address the nature of the periodic signals. The final model and the orbital planetary parameters were obtained by fitting simultaneously the stellar variability and the Keplerian signal using a Gaussian process regression and following a Bayesian criterion. Results. The HARPS-N RV periodic signals around 40 d and 100 d have counterparts at the same frequencies in HARPS-N activity indicators and photometric light curves. Then we attribute these periodicities to stellar activity the former period being likely associated with the stellar rotation. GJ 720 A shows the most significant signal at 19.466$\pm$0.005 d with no counterparts in any stellar activity indices. We hence ascribe this RV signal, having a semiamplitude of 4.72$\pm$0.27 m/s , to the presence of a sub-Neptune mass planet. The planet GJ 720 Ab has a minimum mass of 13.64$\pm$0.79 M$_{\oplus}$, it is in circular orbit at 0.119$\pm$0.002 AU from its parent star, and lies inside the inner boundary of the HZ around its parent star

Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection?

Indexación: Web of Science.Background: For several decades now an antagonism between Trypanosoma cruzi infection and tumor development has been detected. The molecular basis of this phenomenon remained basically unknown until our proposal that T. cruzi Calreticulin (TcCRT), an endoplasmic reticulum-resident chaperone, translocated-externalized by the parasite, may mediate at least an important part of this effect. Thus, recombinant TcCRT (rTcCRT) has important in vivo antiangiogenic and antitumor activities. However, the relevant question whether the in vivo antitumor effect of T. cruzi infection is indeed mediated by the native chaperone (nTcCRT), remains open. Herein, by using specific modified anti-rTcCRT antibodies (Abs), we have neutralized the antitumor activity of T. cruzi infection and extracts thereof, thus identifying nTcCRT as a valid mediator of this effect. Methods: Polyclonal anti-rTcCRT F(ab')(2) Ab fragments were used to reverse the capacity of rTcCRT to inhibit EAhy926 endothelial cell (EC) proliferation, as detected by BrdU uptake. Using these F(ab')(2) fragments, we also challenged the capacity of nTcCRT, during T. cruzi infection, to inhibit the growth of an aggressive mammary adenocarcinoma cell line (TA3-MTXR) in mice. Moreover, we determined the capacity of anti-rTcCRT Abs to reverse the antitumor effect of an epimastigote extract (EE). Finally, the effects of these treatments on tumor histology were evaluated. Results: The rTcCRT capacity to inhibit ECs proliferation was reversed by anti-rTcCRT F(ab')(2) Ab fragments, thus defining them as valid probes to interfere in vivo with this important TcCRT function. Consequently, during infection, these Ab fragments also reversed the in vivo experimental mammary tumor growth. Moreover, anti-rTcCRT Abs also neutralized the antitumor effect of an EE, again identifying the chaperone protein as an important mediator of this anti mammary tumor effect. Finally, as determined by conventional histological parameters, in infected animals and in those treated with EE, less invasive tumors were observed while, as expected, treatment with F(ab')(2) Ab fragments increased malignancy. Conclusion: We have identified translocated/externalized nTcCRT as responsible for at least an important part of the anti mammary tumor effect of the chaperone observed during experimental infections with T. cruzi.http://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2764-

The Antioxidant Potential of the Mediterranean Diet in Patients at High Cardiovascular Risk: An In-Depth Review of the PREDIMED

Cardiovascular disease (CVD) is the leading global cause of death. Diet is known to be important in the prevention of CVD. The PREDIMED trial tested a relatively low-fat diet versus a high-fat Mediterranean diet (MedDiet) for the primary prevention of CVD. The resulting reduction of the CV composite outcome resulted in a paradigm shift in CV nutrition. Though many dietary factors likely contributed to this effect, this review focuses on the influence of the MedDiet on endogenous antioxidant systems and the effect of dietary polyphenols. Subgroup analysis of the PREDIMED trial revealed increased endogenous antioxidant and decreased pro-oxidant activity in the MedDiet groups. Moreover, higher polyphenol intake was associated with lower incidence of the primary outcome, overall mortality, blood pressure, inflammatory biomarkers, onset of new-onset type 2 diabetes mellitus (T2DM), and obesity. This suggests that polyphenols likely contributed to the lower incidence of the primary event in the MedDiet groups. In this article, we summarize the potential benefits of polyphenols found in the MedDiet, specifically the PREDIMED cohort. We also discuss the need for further research to confirm and expand the findings of the PREDIMED in a non-Mediterranean population and to determine the exact mechanisms of action of polyphenols

Previous SARS-CoV-2 Infection Increases B.1.1.7 Cross-Neutralization by Vaccinated Individuals

With the spread of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to assess the protection conferred by both previous infections and current vaccination. Here we tested the neutralizing activity of infected and/or vaccinated individuals against pseudoviruses expressing the spike of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1), the D614G mutant and the B.1.1.7 variant. Our data show that parameters of natural infection (time from infection and nature of the infecting variant) determined cross-neutralization. Uninfected vaccinees showed a small reduction in neutralization against the B.1.1.7 variant compared to both the WH1 strain and the D614G mutant. Interestingly, upon vaccination, previously infected individuals developed more robust neutralizing responses against B.1.1.7, suggesting that vaccines can boost the neutralization breadth conferred by natural infection