Motif Berperilaku Kepala Desa Pada Penyelenggaraan Pemerintahan Desa di Kabupaten Minahasa Selatan

The behavior of the village chief is expected to build the image quality public services, but in reality the implementation of public services have not been able to walk properly due to the unavailability of the service personnel are professional, dedicated, accountable and responsive and loyal to his duties as civil servants and public servants. By using a qualitative approach was found that social status (achievement, prestige / status position and power / power, and needs (income and appreciation) are two underlying motives in the village head behaves In Governance in South Minahasa Regency Village. Loyalty and professional as media which can bridge the social status and the need to make quality services. Keywords: Motive behavior, social status, Supplies, Service

The Impact of Social Distancing and Physical Distancing Policies in the Management of Artificial Tourism Objects

Social distancing and physical distancing policies are actions taken by the government to stop the spread of Covid 19. In its implementation, it has had an impact on the management of artificial tourism objects. The research data was collected directly through the interview process to the managers of artificial tourism objects and tourists directly at the research site. Data collection was also carried out for the government and the Covid 19 task force using the google form. Data was also collected secondaryly by accessing Google Scholar. The presentation of the data in this study was carried out in a descriptive-analytic manner and deductively drawing conclusions. Research findings that the application of social distancing and physical distancing on artificial tourism objects have had a direct or indirect impact on the economic and social aspects

KETERSEDIAAN SARANA AKSESIBILITAS PENYANDANG DISABILITAS di PUSAT LAYANAN PUBLIK PEMERINTAH KOTA MANADO

Persons with Disabilities have the right to access public facilities. However, until now there are still very few facilities that can support their activities in public service centers such as government offices. The right to accessibility is part of human rights and is regulated based on state policy. This study aims to analyze the availability of facilities or facilities related to disability accessibility in Manado city government public service centers. The research design used is a qualitative case study approach. Data was collected through direct interviews and interviews using new technological tools (zoom) adapted to the situation of the informants, as well as observations at Manado City Government public service centers to identify the availability of facilities. Data analysis used an interactive analysis model with the stages of data reduction, data display and conclusion drawing/verification. The research findings are that the accessibility of persons with disabilities at the Manado City Government Service Center in terms of the availability of facilities is not in accordance with the policy mandate. The facilities available to support the delivery of services in the fields of population administration, health and education in the City of Manado are general in nature and intended for all people. Not specifically available for persons with disabilities or people with special needs. The main cause of this is due to non-compliance in implementing policies. Another contributing factor is that the government has not made people with special needs a priority in service

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field