161 research outputs found

    Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

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    Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and accurately identify bacteria in human blood samples by combining optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and efficient immunoaffinity enrichment/separation. A library of bacteria reference mass spectra at different cell numbers was firstly built. Due to a reduced sample spot size, the reference spectra could be obtained from as few as 10 to 10(2) intact bacterial cells. Bacteria in human blood samples were then extracted using antibodies-modified magnetic beads for MS fingerprinting. By comparing the sample spectra with the reference spectra based on a cosine correlation, bacteria with concentrations as low as 500 cells per mL in blood serum and 8000 cells per mL in whole blood were identified. The proposed method was further applied to positive clinical blood cultures (BCs) provided by a local hospital, where Escherichia coli and Staphylococcus aureus were identified. Because of the method's high sensitivity, the BC time required for diagnosis can be greatly reduced. As a proof of concept, whole blood spiked with a low initial concentration (10(2) or 10(3) cells per mL) of bacteria was cultured in commercial BC bottles and analysed by the developed method after different BC times. Bacteria were successfully identified after 4 hours of BC. Therefore, an entire diagnostic process could be accurately accomplished within half a day using the newly developed method, which could facilitate the timely determination of appropriate anti-bacterial therapy and decrease the risk of mortality from bloodstream infections

    The accelerated scaling attractor solution of the interacting agegraphic dark energy in Brans-Dicke theory

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    We investigate the interacting agegraphic dark energy in Brans-Dicke theory and introduce a new series general forms of dark sector coupling. As examples, we select three cases involving a linear interaction form (Model I) and two nonlinear interaction form (Model II and Model III). Our conclusions show that the accelerated scaling attractor solutions do exist in these models. We also find that these interacting agegraphic dark energy modes are consistent with the observational data. The difference in these models is that nonlinear interaction forms give more approached evolution to the standard Λ\LambdaCDM model than the linear one. Our work implies that the nonlinear interaction forms should be payed more attention.Comment: 9 pages, 10 figures, accepted in Eur. Phys. J.

    Combined constraints on modified Chaplygin gas model from cosmological observed data: Markov Chain Monte Carlo approach

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    We use the Markov Chain Monte Carlo method to investigate a global constraints on the modified Chaplygin gas (MCG) model as the unification of dark matter and dark energy from the latest observational data: the Union2 dataset of type supernovae Ia (SNIa), the observational Hubble data (OHD), the cluster X-ray gas mass fraction, the baryon acoustic oscillation (BAO), and the cosmic microwave background (CMB) data. In a flat universe, the constraint results for MCG model are, Ωbh2=0.022630.00162+0.00184\Omega_{b}h^{2}=0.02263^{+0.00184}_{-0.00162} (1σ1\sigma) 0.00195+0.00213^{+0.00213}_{-0.00195} (2σ)(2\sigma), Bs=0.77880.0723+0.0736B_{s}=0.7788^{+0.0736}_{-0.0723} (1σ1\sigma) 0.0904+0.0918^{+0.0918}_{-0.0904} (2σ)(2\sigma), α=0.10790.2539+0.3397\alpha=0.1079^{+0.3397}_{-0.2539} (1σ1\sigma) 0.2911+0.4678^{+0.4678}_{-0.2911} (2σ)(2\sigma), B=0.001890.00756+0.00583B=0.00189^{+0.00583}_{-0.00756} (1σ1\sigma) 0.00915+0.00660^{+0.00660}_{-0.00915} (2σ)(2\sigma), and H0=70.7113.142+4.188H_{0}=70.711^{+4.188}_{-3.142} (1σ1\sigma) 4.149+5.281^{+5.281}_{-4.149} (2σ)(2\sigma).Comment: 12 pages, 1figur

    Direct Measurements of the Branching Fractions for D0Ke+νeD^0 \to K^-e^+\nu_e and D0πe+νeD^0 \to \pi^-e^+\nu_e and Determinations of the Form Factors f+K(0)f_{+}^{K}(0) and f+π(0)f^{\pi}_{+}(0)

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    The absolute branching fractions for the decays D0Ke+νeD^0 \to K^-e ^+\nu_e and D0πe+νeD^0 \to \pi^-e^+\nu_e are determined using 7584±198±3417584\pm 198 \pm 341 singly tagged Dˉ0\bar D^0 sample from the data collected around 3.773 GeV with the BES-II detector at the BEPC. In the system recoiling against the singly tagged Dˉ0\bar D^0 meson, 104.0±10.9104.0\pm 10.9 events for D0Ke+νeD^0 \to K^-e ^+\nu_e and 9.0±3.69.0 \pm 3.6 events for D0πe+νeD^0 \to \pi^-e^+\nu_e decays are observed. Those yield the absolute branching fractions to be BF(D0Ke+νe)=(3.82±0.40±0.27)BF(D^0 \to K^-e^+\nu_e)=(3.82 \pm 0.40\pm 0.27)% and BF(D0πe+νe)=(0.33±0.13±0.03)BF(D^0 \to \pi^-e^+\nu_e)=(0.33 \pm 0.13\pm 0.03)%. The vector form factors are determined to be f+K(0)=0.78±0.04±0.03|f^K_+(0)| = 0.78 \pm 0.04 \pm 0.03 and f+π(0)=0.73±0.14±0.06|f^{\pi}_+(0)| = 0.73 \pm 0.14 \pm 0.06. The ratio of the two form factors is measured to be f+π(0)/f+K(0)=0.93±0.19±0.07|f^{\pi}_+(0)/f^K_+(0)|= 0.93 \pm 0.19 \pm 0.07.Comment: 6 pages, 5 figure

    Measurements of J/psi Decays into 2(pi+pi-)eta and 3(pi+pi-)eta

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    Based on a sample of 5.8X 10^7 J/psi events taken with the BESII detector, the branching fractions of J/psi--> 2(pi+pi-)eta and J/psi-->3(pi+pi-)eta are measured for the first time to be (2.26+-0.08+-0.27)X10^{-3} and (7.24+-0.96+-1.11)X10^{-4}, respectively.Comment: 11 pages, 6 figure

    BESII Detector Simulation

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    A Monte Carlo program based on Geant3 has been developed for BESII detector simulation. The organization of the program is outlined, and the digitization procedure for simulating the response of various sub-detectors is described. Comparisons with data show that the performance of the program is generally satisfactory.Comment: 17 pages, 14 figures, uses elsart.cls, to be submitted to NIM

    Measurement of branching fractions for the inclusive Cabibbo-favored ~K*0(892) and Cabibbo-suppressed K*0(892) decays of neutral and charged D mesons

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    The branching fractions for the inclusive Cabibbo-favored ~K*0 and Cabibbo-suppressed K*0 decays of D mesons are measured based on a data sample of 33 pb-1 collected at and around the center-of-mass energy of 3.773 GeV with the BES-II detector at the BEPC collider. The branching fractions for the decays D+(0) -> ~K*0(892)X and D0 -> K*0(892)X are determined to be BF(D0 -> \~K*0X) = (8.7 +/- 4.0 +/- 1.2)%, BF(D+ -> ~K*0X) = (23.2 +/- 4.5 +/- 3.0)% and BF(D0 -> K*0X) = (2.8 +/- 1.2 +/- 0.4)%. An upper limit on the branching fraction at 90% C.L. for the decay D+ -> K*0(892)X is set to be BF(D+ -> K*0X) < 6.6%

    Measurements of the Mass and Full-Width of the ηc\eta_c Meson

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    In a sample of 58 million J/ψJ/\psi events collected with the BES II detector, the process J/ψγηc\psi\to\gamma\eta_c is observed in five different decay channels: γK+Kπ+π\gamma K^+K^-\pi^+\pi^-, γπ+ππ+π\gamma\pi^+\pi^-\pi^+\pi^-, γK±KS0π\gamma K^\pm K^0_S \pi^\mp (with KS0π+πK^0_S\to\pi^+\pi^-), γϕϕ\gamma \phi\phi (with ϕK+K\phi\to K^+K^-) and γppˉ\gamma p\bar{p}. From a combined fit of all five channels, we determine the mass and full-width of ηc\eta_c to be mηc=2977.5±1.0(stat.)±1.2(syst.)m_{\eta_c}=2977.5\pm1.0 ({stat.})\pm1.2 ({syst.}) MeV/c2c^2 and Γηc=17.0±3.7(stat.)±7.4(syst.)\Gamma_{\eta_c} = 17.0\pm3.7 ({stat.})\pm7.4 ({syst.}) MeV/c2c^2.Comment: 9 pages, 2 figures and 4 table. Submitted to Phys. Lett.

    Study of J/ψωK+KJ/\psi \to \omega K^+K^-

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    New data are presented on J/ψωK+KJ/\psi \to \omega K^+K^- from a sample of 58M J/ψJ/\psi events in the upgraded BES II detector at the BEPC. There is a conspicuous signal for f0(1710)K+Kf_0(1710) \to K^+K^- and a peak at higher mass which may be fitted with f2(2150)KKˉf_2(2150) \to K\bar K. From a combined analysis with ωπ+π\omega \pi ^+ \pi ^- data, the branching ratio BR(f0(1710)ππ)/BR(f0(1710)KKˉ)BR(f_0(1710)\to\pi\pi)/BR(f_0(1710) \to K\bar K) is <0.11< 0.11 at the 95% confidence level.Comment: 11 pages, 5 figures. Submitted to Phys. Lett.
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