Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato.

Auxin response factors (ARFs) are involved in auxin-mediated transcriptional regulation in plants. In this study, we performed functional characterization of SlARF6A in tomato. SlARF6A is located in the nucleus and exhibits transcriptional activator activity. Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants, whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type (WT) plants. Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants. Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars, whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits. RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism, photosynthesis and sugar metabolism. SlARF6A directly bound to the promoters of SlGLK1, CAB, and RbcS genes and positively regulated the expression of these genes. Overexpression of SlARF6A also inhibited fruit ripening and ethylene production, whereas downregulation of SlARF6A increased fruit ripening and ethylene production. SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression. Taken together, these results expand our understanding of ARFs with regard to photosynthesis, sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops

A feature fusion optimization algorithm for predicting the remaining useful life of lithium-ion batteries

As the percentage of lithium-ion batteries as in a system for storing energy gradually rises, accidents brought by the deterioration of battery performance continue to occur. The solution to guaranteeing the steady operation of this system is learning how to precisely forecast the lithium-ion batteries’ remaining useful life (RUL). A prediction framework that combines elements of incremental capacity analysis (ICA) and electrochemical impedance spectroscopy (EIS) is proposed to address issues of RUL. The framework initially examines the charging and discharging features of the battery before establishing a mapping association between fusion features and RUL using convolutional neural network (CNN) and improved long short-term memory network (ILSTM). The parameters of the improved particle swarm optimization algorithm (IPSO) are optimized to build the IPSO-CNN-ILSTM model by modifying updating rules of the inertia weight and learning factor of the particle swarm optimization (PSO) algorithm to improve its optimization ability. Lastly, numerical outcomes of the NASA PCoE datasets confirm this method’s applicability and efficacy

Determination of Mass Transfer Coefficient of Methane in Heavy Oil-Saturated Unconsolidated Porous Media Using Constant-Pressure Technique

Measuring the solubility and diffusivity of methane in porous media saturated with oil is critical for analyzing the process of solvent-based recovery of heavy oil reservoir. In this study, a modified pressure-pulse decay (PPD) method is presented to measure the mass transfer coefficients of methane in porous media saturated with heavy oil as well as the solubility and diffusivity of methane in bulk oil under the same experimental conditions as those used in a comparison. The experiments are conducted under constant boundary pressure in a PVT cell, and the pressure is controlled using an auto pump, which continuously compresses the volume of an intermediate container. The accumulated volume change instead of the pressure change is recorded with time. The nonequilibrium boundary condition (BC) model is used to analyze the experimental process. The general solution of the mathematical model is derived using the Laplace transform and the approximate analytical solution of the accumulated dissolved gas is further presented for post processing of the experimental data. The mass transfer coefficients are estimated through using a multilevel single-linkage (MLSL) method to match the approximate solution with the recorded experimental data. The estimation of the parameters shows that the mass transfer coefficients are pressure dependent and that a high boundary pressure contributes to the mass transfer of methane in heavy oil due to a reduction in viscosity or density. The interface mass transfer coefficient (<i>k</i>_int) and interface saturated concentration (<i>c</i>_int) in porous media saturated with oil and bulk oil are almost the same under the same experimental conditions. The sensitivity analysis shows that the increase of the effective diffusion coefficient (<i>D</i>_eff) and <i>k</i>_int contributes to enhancing the rate of mass transfer in the oil phase and that Henry’s law constant (<i>H</i>) has no effect on the equilibrium time but only affects the initial saturated concentration at the interface or the total dissolved gas. The modified PPD method is robust, efficient, and easy to use in the laboratory

Phase Behavior for Poly(vinylacetate) + Carbon Dioxide + Cosolvent Ternary Systems

Experimental cloud point and bubble point data of ternary mixtures for poly­(vinylacetate) (PVAc) and cosolvents in carbon dioxide (CO₂) are studied using the supercritical CO₂ phase behavior device. The cloud point and bubble point pressures are determined by measuring the resistance variation of photoresistance. Acetate acid and ethyl acetate have been selected as the cosolvents. These systems show phase behavior over a temperature range of 308.2–338.2 K and pressure of up to 50 MPa. The transition point pressure increases as the temperature increases. The transition point isopleths for the PVAc + CO₂ + cosolvent ternary systems show upper critical solution pressure types. The cloud point and bubble point curves for the PVAc + CO₂ + cosolvent systems decrease rapidly as the cosolvent concentration increases. When the mass ratio of PVAc and cosolvents is fixed at 1:7, the transition point pressures increase as CO₂ increases. Compared with acetic acid and ethyl acetate, the transition point pressures of the PVAc + CO₂ mixtures, containing the two cosolvents, show that the pressure for acetate acid is less than that of ethyl acetate under the same conditions. The phase behaviors of these ternary systems are calculated using the perturbed-chain statistical associating fluid theory equation of state by adjusting the binary interaction parameter <i>k</i>_ij. Our models are in very good agreement with the experimental data

SKP-SC-EVs Mitigate Denervated Muscle Atrophy by Inhibiting Oxidative Stress and Inflammation and Improving Microcirculation

Denervated muscle atrophy is a common clinical disease that has no effective treatments. Our previous studies have found that oxidative stress and inflammation play an important role in the process of denervated muscle atrophy. Extracellular vesicles derived from skin precursor-derived Schwann cells (SKP-SC-EVs) contain a large amount of antioxidants and anti-inflammatory factors. This study explored whether SKP-SC-EVs alleviate denervated muscle atrophy by inhibiting oxidative stress and inflammation. In vitro studies have found that SKP-SC-EVs can be internalized and caught by myoblasts to promote the proliferation and differentiation of myoblasts. Nutrient deprivation can cause myotube atrophy, accompanied by oxidative stress and inflammation. However, SKP-SC-EVs can inhibit oxidative stress and inflammation caused by nutritional deprivation and subsequently relieve myotube atrophy. Moreover, there is a remarkable dose-effect relationship. In vivo studies have found that SKP-SC-EVs can significantly inhibit a denervation-induced decrease in the wet weight ratio and myofiber cross-sectional area of target muscles. Furthermore, SKP-SC-EVs can dramatically inhibit highly expressed Muscle RING Finger 1 and Muscle Atrophy F-box in target muscles under denervation and reduce the degradation of the myotube heavy chain. SKP-SC-EVs may reduce mitochondrial vacuolar degeneration and autophagy in denervated muscles by inhibiting autophagy-related proteins (i.e., PINK1, BNIP3, LC3B, and ATG7). Moreover, SKP-SC-EVs may improve microvessels and blood perfusion in denervated skeletal muscles by enhancing the proliferation of vascular endothelial cells. SKP-SC-EVs can also significantly inhibit the production of reactive oxygen species (ROS) in target muscles after denervation, which indicates that SKP-SC-EVs elicit their role by upregulating Nrf2 and downregulating ROS production-related factors (Nox2 and Nox4). In addition, SKP-SC-EVs can significantly reduce the levels of interleukin 1β, interleukin-6, and tumor necrosis factor α in target muscles. To conclude, SKP-SC-EVs may alleviate the decrease of target muscle blood perfusion and passivate the activities of ubiquitin-proteasome and autophagy-lysosome systems by inhibiting oxidative stress and inflammatory response, then reduce skeletal muscle atrophy caused by denervation. This study not only enriches the molecular regulation mechanism of denervated muscle atrophy, but also provides a scientific basis for SKP-SC-EVs as a protective drug to prevent and treat muscle atrophy

Identification of Prognostic and Therapeutic Biomarkers among FAM83 Family Members for Pancreatic Ductal Adenocarcinoma

Family with sequence similarity 83 (FAM83) members were shown recently to have oncogenic effect in a variety of cancer types, but the biological roles and prognostic value of FAM83 family in pancreatic ductal adenocarcinoma remain unknown. In the current study, the clinical significance and molecular function of the FAM83 family were assessed by multiple bioinformatics analysis. Besides, potential associations between differentially expressed genes (DEGs) of FAM83 family and antitumor immunity were evaluated using TIMER and TISIDB analyses. As the results show, FAM83A, FAM83D, FAM83E, and FAM83H were significantly upregulated in PDAC and were identified as DEGs. Higher expression of FAM83A, FAM83B, FAM83D, FAM83E, and FAM83H were associated with advanced tumor stage or worse patient prognosis. Importantly, the overexpression of DEGs was found to be significantly correlated with activated KRAS and loss of SMAD4, which are important drivers for PDAC. Further, FAM83A, FAM83D, and FAM83H were associated with CD8+ T cell, Gamma Delta T cell, and CD4+ T cell infiltration in PDAC and FAM83H was found closely correlated with some immunomodulators including immunoinhibitors, immunostimulators, and MHC molecules. In conclusion, FAM83A, FAM83D, FAM83E, and FAM83H have significant prognostic value in PDAC and they may play important roles in regulating tumor progression and the immune cell infiltration

A Novel Prognostic Nomogram for Gallbladder Cancer after Surgical Resection: A Single-Center Experience

Background. Gallbladder cancer (GBC), which accounts for more than 80% of biliary tract malignancies, has a poor prognosis with an overall 5-year survival less than 10%. The study aimed to identify risk factors and develop a predictive model for GBC following surgical resection. Methods. 98 GBC patients who underwent surgical resection from Guangdong Provincial People’s Hospital were enrolled in the study. Cox-regression analysis was performed to identify significant prognostic factors. A nomogram was constructed and Harrell’s concordance index, calibration plot, and decision cure analysis were used to evaluate the discrimination and calibration of the nomogram. Results. Liver resection, tumor size, perineural invasion, surgical margin, and liver invasion were identified as independent risk factors for overall survival (OS) in GBC patients who underwent surgical resection. Based on the selected risk factors, a novel nomogram was constructed. The C-index of the nomogram was 0.777, which was higher than the American Joint Committee on Cancer (AJCC) staging system (0.724) and Nevin staging system (0.659). Decision cure analysis revealed that the nomogram had a better net benefit and the calibration curves for the 1-, 3-, and 5-year survival probabilities were also well matched with the actual survival rates. Lastly, high-risk GBC were stratified based on the scores of the nomogram and we found high-risk GBC were associated with both worse OS and disease-free survival (DFS). Conclusion. We developed a nomogram showing a better predictive capacity for patients’ survival of resected GBC than the AJCC staging systems. The established model may help to stratify high-risk GBC and facilitate decision-making in the clinic

Control of fruit softening and Ascorbic acid accumulation by manipulation of SlIMP3 in tomato

Postharvest deterioration is among the major challenges for the fruit industry. Regulation of the fruit softening rate is an effective strategy for extending shelf-life and reducing the economic losses due postharvest deterioration. The tomato myoinositol monophosphatase 3 gene SlIMP3, which showed highest expression level in fruit, was expressed and purified. SlIMP3 demonstrated high affinity with the L-Gal 1-P and D-Ins 3-P, and acted as a bifunctional enzyme in the biosynthesis of AsA and myoinositol. Overexpression of SlIMP3 not only improved AsA and myoinositol content, but also increased cell wall thickness, improved fruit firmness, delayed fruit softening, decreased water loss, and extended shelf-life. Overexpression of SlIMP3 also increased uronic acid, rhamnose, xylose, mannose, and galactose content in cell wall of fruit. Treating fruit with myoinositol obtained similar fruit phenotypes of SlIMP3-overexpressed fruit, with increased cell wall thickness and delayed fruit softening. Meanwhile, overexpression of SlIMP3 conferred tomato fruit tolerance to Botrytis cinerea. The function of SlIMP3 in cell wall biogenesis and fruit softening were also verified using another tomato species, Ailsa Craig (AC). Overexpression of SlDHAR in fruit increased AsA content, but did not affect the cell wall thickness or fruit firmness and softening. The results support a critical role for SlIMP3 in AsA biosynthesis and cell wall biogenesis, and provide a new method of delaying tomato fruit softening, and insight into the link between AsA and cell wall metabolism

R2R3 MYB‐dependent auxin signalling regulates trichome formation, and increased trichome density confers spider mite tolerance on tomato

Unicellular and multicellular tomato trichomes function as mechanical and chemical barriers against herbivores. Auxin treatment increased the formation of II, V and VI type trichomes in tomato leaves. The auxin response factor gene SlARF4, which was highly expressed in II, V and VI type trichomes, positively regulated the auxin-induced formation of II, V and VI type trichomes in the tomato leaves. SlARF4 overexpression plants with high densities of these trichomes exhibited tolerance to spider mites. Two R2R3 MYB genes, SlTHM1 and SlMYB52, were directly targeted and inhibited by SlARF4. SlTHM1 was specifically expressed in II and VI type trichomes and negatively regulated the auxin-induced formation of II and VI type trichomes in the tomato leaves. SlTHM1 down-regulation plants with high densities of II and VI type trichomes also showed tolerance to spider mites. SlMYB52 was specifically expressed in V type trichomes and negatively regulated the auxin-induced formation of V type trichome in the tomato leaves. The regulation of SlARF4 on the formation of II, V and VI type trichomes depended on SlTHM1 and SlMYB52, which directly targeted cyclin gene SlCycB2 and increased its expression. In conclusion, our data indicates that the R2R3 MYB-dependent auxin signalling pathway regulates the formation of II, V and VI type trichomes in tomato leaves. Our study provides an effective method for improving the tolerance of tomato to spider mites