Human Pappilomavirus Genotype in Cervical Tissue of Patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18

Perbaikan Konstruksi Bubu Elver Skala Laboratorium (Correction of Elver Trap Contruction in Laboratory Scale)

PVC trap is used by fisherman in Java Island, southern waters to capture elver, or juvenileeels measuring < 10 gr. The problems are that trap catches too less and elver in injured condition,while buyers need a lot of good elvers for cultivating. This study tried to fix a trap construction sothat function more effective and doesn't hurt elver. All experiments were conducted in the FishingGear Laboratory, Bogor Agricultural University. Three sections of traps were examined were therear cover of trap construction, entrance of trap construction and application two doors of traps.Furthermore, new traps made by three criteria were obtained. The entire test was conducted in theexperimental tank containing between 30-100 elvers. The test was done as much as 20-25 timeswith 20 minutes soaking time. The results showed that more elvers enter the trap which does notseal, the entrance was made from nets and had two doors of the trap. The construction trap basedon three criteria was more effective which could trap 355 elvers or 6.12 times more than thefisherman's trap (58 elvers)

Oxalate and silica contents of seven varieties of Napier grass (Pennisetum purpureum)

Oxalate and silica are considered antinutrients. Large quantities of oxalate and silica in plants can interfere with the uptake of essential minerals in ruminants. Therefore, the aim of this study was to compare the total silica and oxalate contents of seven varieties of Napier grass to find out which is best for cultivation. Taiwan, Zanzibar, Pakchong, Purple, Kobe, Indian, and Dwarf Napier grass were grown in a completely randomized design with three replications to determine their soluble oxalate, total oxalate, and silica contents. Plants were harvested at two months of plant maturity. Whole plant of the Dwarf Napier grass contained significantly higher soluble oxalate content than tall varieties. Total oxalate content in whole plant differed significantly among varieties. Dwarf showed the highest total oxalate content (3.23% dry matter (DM)) followed by Kobe (2.61%), Zanzibar (2.60%), Purple (2.44%), Taiwan (2.43%), Indian (2.15%), and Pakchong (1.95%). Regardless of variety, leaf tissue contained significantly higher soluble oxalate and total oxalate than stem tissue. There were no differences in silica content among them. In conclusion, the tall varieties could produce lower levels of soluble oxalate than the Dwarf variety, whereas silica content might not vary among them.Keywords: botanical fractions, mineral bioavailability, ruminan

Penguatan Cahaya Pada Bagan Menggunakan Reflektor Kerucut Sebagai Upaya Meningkatkan Hasil Tangkapan Cumi-cumi (Light Strengthening on Lift Net with Conical Reflectors to Squid Catch Improvement)

Study of light strengthening on lift net with conical reflectors was conducted in Kao Bay waters. Three units of lift net were operated 14 nights at the full moon condition. Every lift nets were completed with three different kind of lamp cover, called tudung, reflector αr23,3o and αr32,6o. Fishing operation time of lift net were divided into two intervals of time i.e 20.00-01.00 and 01.00-05.00 WIT (East Indonesian Timezone). Total yield of lift net with reflector αr23,3o was 5.774 kg (41,45%), while that of the lift net with reflector αr 32,6o was 4.977 kg (35,72%), and that of total yield of the lift net with tudung was 3.180 kg (22,83 %). Fishing operation time of lift net at 01.00-05.00 WIT (East Indonesian Timezone) produced 12.661 kg (91 %) weight of total catch, higher than fishing operation of lift net at 20.00-01.00 am that produced 1.270 kg (9 %) weight of total catch. However, statistical analysis concluded that design of the reflector did not significantly affect the catch per trip (Pvalue > 0,05) while fishing time significantly affected the catch per trip (Pvalue < 0,05)

Projeto-piloto de promoção comercial de frutas da produção integrada: fundamentos e resultados.

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Essai d'infection de chèvres tchadienne par le virus de la rhinotrachéite infectieuse bovine. Enquête sérologique dans l'Ouest Tchadien

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Pecuária de corte no Pantanal brasileiro: realidade e perspectivas futuras de melhoramento.

Apesar da pecuária de corte estar presente no Pantanal à mais de duzentos anos, até o momento, não foi estabelecido um programa de melhoramento genético animal para a região que contemplasse as diferentes condições de manejo e de ambiente no processo seletivo dos bovinos de corte. Um programa desta natureza envolveria, melhorias no manejo geral do rebanho, maior cuidado com as práticas de sanidade animal e gestão mais eficiente da atividade como um todo.bitstream/CPAP/56028/1/DOC93.pdfFormato Eletrônic

Skrining Kanker Serviks Dengan Pemeriksaan Pap Smear Di Puskesmas Tanah Kali Kedinding Surabaya Dan Rumah Sakit Mawadah Mojokerto

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks