6 research outputs found
Caracterização genotĂpica dos isolados de Escherichia coli provenientes de frangos de corte
Bioactive properties of phenolic compounds in physalis fruits
Physalis or cape gooseberry (Physalis peruviana L.) is believed to be rich in phenolic compounds, which are synthesized in plant tissues as a response to stress, and many of these compounds have biological properties with putative health benefits. The present work intended to study some bioactive properties of physalis related to the total phenolic compounds content and antioxidant activity, as well as their bioaccessibility along the gastrointestinal tract, by using in vitro enzymatic simulators. Physalis fruits were submitted to extraction, following three methods with different combinations of solvents, which were then analysed for total phenols, antioxidant activity and bioaccessibility. The results showed that the bioaccessibility of total phenols was reduced when reaching the intestine to about half of the original amount, while the bioaccessibility of antioxidant activity was reduced to only one fourth also when reaching the intestine. Some differences were observed for different extracts but not when the sample was used fresh or freeze-dried. In conclusion, these ratios have to be considered when expecting bioactivities through the ingestion of physalis fruits.info:eu-repo/semantics/publishedVersio
Salmonella spp. em carcaças, carne mecanicamente separada, lingĂŒiças e cortes comerciais de frango Salmonella spp. in carcasses, mechanically deboned meat, sausages and chicken meat
Alimentos de origem animal representam papel fundamental na epidemiologia das salmoneloses humanas. Apesar dos avanços tecnolĂłgicos, a carne de frango ainda Ă© passĂvel de contaminação bacteriana, especialmente por microrganismos do gĂȘnero Salmonella, que podem encontrar-se albergados no trato intestinal ou em outra parte do corpo das aves. O presente trabalho objetivou pesquisar a ocorrĂȘncia de Salmonella em carne de frango e derivados procedentes da regiĂŁo Nordeste do Estado de SĂŁo Paulo. Foram analisadas, atravĂ©s do mĂ©todo convencional de cultivo, 45 amostras de carcaças, 60 de carne mecanicamente separada (CMS), 25 de lingĂŒiça de frango, 20 de peito, e 15 de coxa e sobre-coxa. Salmonella spp. foi encontrada em 13,3% (6/45) das carcaças, 25% (15/60) das amostras de CMS, 16% (4/25) das lingĂŒiças, 30% (6/20) dos peitos e 13,3% (2/15) das coxas e sobre-coxas analisadas. Do total de 165 amostras analisadas, 33 (20%) apresentaram contaminação por Salmonella estando, portanto, imprĂłprias para o consumo conforme legislação brasileira.<br>Food of animal origin represents an important role in the epidemiology of human salmonellosis. In spite of the technological improvement, the chicken meat is subjected to bacterial contamination, mainly by microorganisms of the genus Salmonella that can be found in the intestinal tract or elsewhere on the chicken body. The aim of this study was to investigate the occurrence of Salmonella in chicken meat and cuts from the Northeast region of SĂŁo Paulo State, Brazil. By conventional cultivation microbiological methods, 45 samples of carcasses, 60 samples of mechanically deboned meat (MDM), 25 samples of chicken sausages, 20 samples of chest, and 15 samples of chicken leg and thigh. Salmonella was found in 13.3% (6/45) of the carcass, 25% (15/60) of the MDM, 16% (4/25) of the sausages, 30% (6/20) of the chests and 13.3% (2/15) of the tight analysed. The results showed that 33 (20%) out of 165 samples were contaminated by Salmonella. Therefore, these samples were inappropriate for consumption, according to the Brazilian legislation
Perfil de sensibilidade antimicrobiana e detecção do gene ISS pela reação em cadeia da polimerase na tipificação de Escherichia coli patogĂȘnica em codornas de corte sob inspeção sanitĂĄria Profile of antimicrobial resistance and detection of iss gene by the polymerase chain reaction in the typification of pathogenic Escherichia coli in meat type quails under sanitary inspection
A patogenicidade das cepas de Escherichia coli estĂĄ relacionada Ă expressĂŁo de fatores de virulĂȘncia encontrados em elementos genĂ©ticos denominados plasmĂdios. O patotipo APEC, responsĂĄvel por diferentes tipos de doenças em aves, pode apresentar o gene iss que aumenta a resistĂȘncia das cepas de E. coli aos efeitos lĂticos do soro, alĂ©m da resistĂȘncia a diversos antimicrobianos. Este estudo foi conduzido para detectar E. coli em traquĂ©ias de codornas destinadas ao abate e avaliar, pela presença do gene iss e o perfil de susceptibilidade antimicrobiana, o potencial patogĂȘnico para aves e humanos dos isolados obtidos. Foram coletadas 180 traquĂ©ias de codornas para detecção de E. coli, determinação do perfil de resistĂȘncia a agentes antimicrobianos e posterior detecção, por reação em cadeia da polimerase (PCR), do gene iss. Das traquĂ©ias analisadas, 8,9 % (16/180) foram positivas para E. coli, sendo obtidos 20 isolados deste agente. A maioria dos isolados foi resistente Ă Tetraciclina (16/20), seguida pela Ceftazidima (13/20) e Ăcido NalidĂxico (12/20), sendo apenas um resistente Ă Amoxicilina. A detecção do gene iss ocorreu em 55% (11/20) dos isolados. A presença do gene iss e a resistĂȘncia a mĂșltiplos antimicrobianos dos isolados obtidos neste estudo pode indicar um possĂvel potencial patogĂȘnico das cepas de E. coli tanto para codornas quanto para outros tipos de aves e animais e mesmo para o ser humano que fique em contato com as mesmas.<br>The pathogenicity of Escherichia coli strains is partially related to the expression of virulence factors genes, present in genetic elements called plasmids. APEC strains responsible for diseases in birds may present the iss gene which increases the resistance of E. coli strains to the lityc effect of the host's serum, besides resistance to several antimicrobials. This study was conduced in order to detect E. coli in tracheae of meat-type quails and to evaluate, by the presence of the iss gene and the profile of antimicrobial susceptibility, the pathogenic potential of the isolated samples for birds and humans. One hundred and eighty tracheae of quails were collected for detection of E. coli, antimicrobial sensitivity tests, and for polymerase chain reaction (PCR), for detection of iss gene. From the examined quails, 8.9 % (16/180) were positive for E. coli, from which 20 strains of this bacterium were obtained. Most of them were resistant to Tetracycline (16/20), followed by Ceftadizime (13/20) and Nalidixic-acid (12/20) and only one isolate was resistant to Amoxicillin. The detection of iss gene occurred in 55% (11/20) of the isolates, indicating that these strains had the potential to be pathogenic not only for quails, but also for other kinds of birds, other animals and even human beings that would be in contact with these E. coli isolates