Metabolic profiling reveals coordinated switches in primary carbohydrate metabolism in grape berry (Vitis vinifera L.), a non-climacteric fleshy fruit

Changes in carbohydrate metabolism during grape berry development play a central role in shaping the final composition of the fruit. The present work aimed to identify metabolic switches during grape development and to provide insights into the timing of developmental regulation of carbohydrate metabolism. Metabolites from central carbon metabolism were measured using high-pressure anion-exchange chromatography coupled to tandem mass spectrometry and enzymatic assays during the development of grape berries from either field-grown vines or fruiting cuttings grown in the greenhouse. Principal component analysis readily discriminated the various stages of berry development, with similar trajectories for field-grown and greenhouse samples. This showed that each stage of fruit development had a characteristic metabolic profile and provided compelling evidence that the fruit-bearing cuttings are a useful model system to investigate regulation of central carbon metabolism in grape berry. The metabolites measured showed tight coordination within their respective pathways, clustering into sugars and sugar-phosphate metabolism, glycolysis, and the tricarboxylic acid cycle. In addition, there was a pronounced shift in metabolism around veraison, characterized by rapidly increasing sugar levels and decreasing organic acids. In contrast, glycolytic intermediates and sugar phosphates declined before veraison but remained fairly stable post-veraison. In summary, these detailed and comprehensive metabolite analyses revealed the timing of important switches in primary carbohydrate metabolism, which could be related to transcriptional and developmental changes within the berry to achieve an integrated understanding of grape berry development. The results are discussed in a meta-analysis comparing metabolic changes in climacteric versus non-climacteric fleshy fruits

A novel approach to modelling and simulating the contact behaviour between a human hand model and a deformable object

A deeper understanding of biomechanical behaviour of human hands becomes fundamental for any human hand-operated Q2 activities. The integration of biomechanical knowledge of human hands into product design process starts to play an increasingly important role in developing an ergonomic product-to-user interface for products and systems requiring high level of comfortable and responsive interactions. Generation of such precise and dynamic models can provide scientific evaluation tools to support product and system development through simulation. This type of support is urgently required in many applications such as hand skill training for surgical operations, ergonomic study of a product or system developed and so forth. The aim of this work is to study the contact behaviour between the operators’ hand and a hand-held tool or other similar contacts, by developing a novel and precise nonlinear 3D finite element model of the hand and by investigating the contact behaviour through simulation. The contact behaviour is externalised by solving the problem using the bi-potential method. The human body’s biomechanical characteristics, such as hand deformity and structural behaviour, have been fully modelled by implementing anisotropic hyperelastic laws. A case study is given to illustrate the effectiveness of the approac

Suppression of two-bounce windows in kink-antikink collisions

We consider a class of topological defects in $(1,1)$-dimensions with a deformed $\phi^4$ kink structure whose stability analysis leads to a Schr\"odinger-like equation with a zero-mode and at least one vibrational (shape) mode. We are interested in the dynamics of kink-antikink collisions, focusing on the structure of two-bounce windows. For small deformation and for one or two vibrational modes, the observed two-bounce windows are explained by the standard mechanism of a resonant effect between the first vibrational and the translational modes. With the increasing of the deformation, the effect of the appearance of more than one vibrational mode is the gradual disappearance of the initial two-bounce windows. The total suppression of two-bounce windows even with the presence of a vibrational mode offers a counterexample from what expected from the standard mechanism. For even larger deformation, some two-bounce windows reappear, but with a non-standard structure.Comment: 13 pages, 6 figure

Analysis of ground potential distribution under lightning current condition

The grounding system of a lightning protection scheme is designed basically to avoid arcing and dangerous step potentials. The grounding impedance of the system varies depending on soil structure and frequency. This paper describes the effect of harmonic impedance (also called frequency dependence of soil) on potential distribution under lightning strike to a metal tower with single grounding path, for different soil types. The results show that the peak value of ground potential rise (GPR) and step voltage (SP) may reach extremely hazardous values even at distances in the order of 90 m from the tower footing, especially when soil resistivity is high. Hence, we emphasise that, in contrast to power grounding, when designing of grounding systems that are meant to handle transient or high frequency currents as well, the frequency dependent soil parameters should be considered to avoid hazardous situations, especially at locations with a high probability of lightning strikes such as metal towers