Antimicrobial Photodynamic Therapy as a Technique for Decontamination of Acrylic Resin Devices Provided by Different Dental Laboratories : Antimicrobial PT for Decontamination of Acrylic Resin Devices

Introduction: Dentures, occlusal splints, surgical guides and orthodontic appliances are examples of acrylic resin devices made in dental laboratories, which must be disinfected and even sterilized before insertion into the oral cavity. This study evaluated the antimicrobial effect of photodynamic therapy (PDT) applied to acrylic resin specimens received from different laboratories.Methods: Three hundred standardized specimens were ordered from six randomly selected laboratories registered in the Council of Dentistry of Ceará (n=50). The PDT consisted in the association of 22 µM erythrosine, as a photosensitizer (P), and a 520-nm LED at 38 J/cm2 (L). The specimens of each laboratory were randomly distributed into five groups: positive control, sterilized with ethylene oxide; negative control, untreated (P-L-); erythrosine control, only stained (P+L-); LED control, only irradiated (P-L+); PDT (P+L+). Then, the specimens were individually sonicated in saline solution; the suspension was diluted, plated on culture mediums (blood agar, sabouraud dextrose agar, and a non-selective chromogenic agar), and incubated for 48 hours at 37°C. Colonyforming-unit (CFU) counts were done and statistical tests of Kruskal-Wallis/Dunn were carried out.Results: The specimens from all laboratories were contaminated with bacteria and yeasts. Staphylococcus aureus, Staphylococcus saprophyticus, Escherichia coli, Enterococcus spp., Klebsiella, and Pseudomonas spp. were identified. The PDT significantly reduced CFU counts (P<0.0001), compared to P-L-.Conclusion: PDT was able to effectively decontaminate the acrylic resin specimens provided by dental laboratories

Alterated lingual frenulum associated with gagging during breastfeeding: case reporta

Objetivo: o presente trabalho tem como objetivo relatar o caso de um paciente recém-nascido prematuro com lesão lingual frênulo associado a episódios de engasgos. Caso Relatório: todos os dados foram obtidos diretamente no discurso serviço de terapia, bem como do hospital do paciente registros. A família autorizou as imagens usadas nesta apresentação. Uma vez que o recém-nascido começou oral alimentação, ele apresentou episódios de engasgos e respiração aflição. A equipe de fonoaudiologia realizou a Avaliação do frênulo lingual proposto por Martinelli e adaptado para recém-nascidos, em outros para observar aspectos funcionais e funcionais e os aspectos sucção não nutritiva e nutritiva. A avaliação concluíram que o frênulo lingual era disfuncional. Quanto à conduta terapêutica, o recém-nascido foi submetido a uma frenulotomia lingual realizada por um cirurgião oral, com apoio do fonoaudiólogo, em um ambiente ambulatorial e sem complicações. A ação interdisciplinar proporciona eficácia dos resultados, baseado no aspecto de que a função de a língua pode ser verificada imediatamente após a cirurgia. Uma semana após a frenulotomia, foi observado a ausência de engasgos, cansaço durante a amamentação, redução do período de pausa e aumento de o poder de sucção. Conclusão: este caso esclarece a discussão sobre frênulo lingual alterado e sua importância para funções essenciais como sucção e deglutição.Objective: the present work aims to report the case of a premature newborn patient with altered lingual frenulum associated with gagging episodes. Case Report: all data was obtained directly in the speech therapy service, as well as from the patient’s hospital records. The family authorized the images used on this presentation. Once the newborn started oral feeding, he presented gagging episodes and respiratory distress. The speech therapy team performed the lingual frenulum evaluation proposed by Martinelli and adapted to newborns, in other to observe anatomical and functional aspects functional aspects and the non-nutritive and nutritive suction. The evaluation concluded that the lingual frenulum was dysfunctional. As to the therapeutic conduct, the newborn was submitted to a lingual frenulotomy performed by an oral surgeon, with speech therapist support, in an outpatient setting and with no complications. The cross-disciplinary action provides effectiveness of the results, based on the aspect that the function of the tongue can be checked immediately after the surgery. One week after the frenulotomy, it was observed the absence of gagging, tiredness during breastfeeding, reduction of the pause period and increase of the suction power. Conclusion: this case clarifies the discussion about disturbed lingual frenulum and its importance to essential functions such as suction and deglutition