On the Disalignment of Interstellar Grains

Several mechanisms have been proposed to explain the alignment of grains with the interstellar magnetic field, including paramagnetic dissipation, radiative torques, and supersonic gas-grain streaming. These must compete with disaligning processes, including randomly directed torques arising from collisions with gas atoms. I describe a novel disalignment mechanism for grains that have a time-varying electric dipole moment and that drift across the magnetic field. Depending on the drift speed, this mechanism may yield a much shorter disalignment timescale than that associated with random gas atom impacts. For suprathermally rotating grains, the new disaligning process may be more potent for carbonaceous dust than for silicate dust. This could result in efficient alignment for silicate grains but poor alignment for carbonaceous grains.Comment: 19 pages, 3 figures, submitted to Ap

Ambulation protocols leading to decreased postoperative complications and hospital stay

Background: In the postoperative course, patients are routinely encouraged to ambulate as frequently as possible. Typically in the hospital this can become burdensome to the staff and often becomes low priority. Patients are also not aware of the frequency and quality of the ambulation that is sufficient in the postoperative period. At present, patients on the surgical floor who are completely independent and without any devices (eg. Oxygen, nasogastric tubes, chest tubes) are freely able to ambulate at will although there is no reliable way to track this progress. Other patients with devices are limited to waiting for nursing or ancillary staff to assist them with securing the devices that they require in the postoperative period. Ambulation has been positively associated with decreased postoperative complications ranging from bowel function to deep venous thrombosis to pneumonia.https://jdc.jefferson.edu/patientsafetyposters/1065/thumbnail.jp

Relation of lineaments to sulfide deposits: Bald Eagle Mountain, Centre County, Pennsylvania

The author has identified the following significant results. Discrete areas of finely-fractured and brecciated sandstone float are present along the crest of Bald Mountain and are commonly sites of sulfide mineralization, as evidenced by the presence of barite and limonite gossans. The frequency distributions of the brecciated float as the negative binomial distribution supports the interpretation of a separate population of intensely fractured material. Such zones of concentrated breccia float have an average width of one kilometer with a range from 0.4 to 1.6 kilometers and were observed in a quarry face to have subvertical dips. Direct spatial correlation of the Landsat-derived lineaments to the fractured areas on the ridge is low; however, the mineralized and fracture zones are commonly assymetrical to the lineament positions. Such a systematic dislocation might result from an inherent bias in the float population or could be the product of the relative erosional resistance of the silicified material in the mineralized areas in relation to the erosionally weak material at the stream gaps

Xenopus Drf1, a Regulator of Cdc7, Displays Checkpoint-dependent Accumulation on Chromatin during an S-phase Arrest

We have cloned a Xenopus Dbf4-related factor named Drf1 and characterized this protein by using Xenopus egg extracts. Drf1 forms an active complex with the kinase Cdc7. However, most of the Cdc7 in egg extracts is not associated with Drf1, which raises the possibility that some or all of the remaining Cdc7 is bound to another Dbf4-related protein. Immunodepletion of Drf1 does not prevent DNA replication in egg extracts. Consistent with this observation, Cdc45 can still associate with chromatin in Drf1-depleted extracts, albeit at significantly reduced levels. Nonetheless, Drf1 displays highly regulated binding to replicating chromatin. Treatment of egg extracts with aphidicolin results in a substantial accumulation of Drf1 on chromatin. This accumulation is blocked by addition of caffeine and by immunodepletion of either ATR or Claspin. These observations suggest that the increased binding of Drf1 to aphidicolin-treated chromatin is an active process that is mediated by a caffeine-sensitive checkpoint pathway containing ATR and Claspin. Abrogation of this pathway also leads to a large increase in the binding of Cdc45 to chromatin. This increase is substantially reduced in the absence of Drf1, which suggests that regulation of Drf1 might be involved in the suppression of Cdc45 loading during replication arrest. We also provide evidence that elimination of this checkpoint causes resumed initiation of DNA replication in both Xenopus tissue culture cells and egg extracts. Taken together, these observations argue that Drf1 is regulated by an intra-S-phase checkpoint mechanism that down-regulates the loading of Cdc45 onto chromatin containing DNA replication blocks

Lineaments and Mineral Occurrences in Pennsylvania

The author has identified the following significant results. A conservative lineament map of Pennsylvania interpreted from ERTS-1 channel 7 (infrared) imagery and Skylab photography was compared with the distribution of known metallic mines and mineral occurrences. Of 383 known mineral occurrences, 116 show a geographical association to 1 km wide lineaments, another 24 lie at the intersection of two lineaments, and one lies at the intersection of three lineaments. The Perkiomen Creek lineament in the Triassic Basin is associated with 9 Cu-Fe occurrences. Six Pb-Zn occurrences are associated with the Tyrone-Mount Union lineament. Thirteen other lineaments are associated with 3, 4, or 5 mineral occurrences each

The Effects of the Spatial Pattern of Defoliation on Regrowth of a Tussock Grass

The influence of the spatial pattern of foliage removal on regrowth was investigated in the field with a tussock grass, Agropyron desertorum. Tussocks were hand clipped in different spatial patterns that represented extremes of defoliation patterns which could be inflicted by natural herbivores. All defoliated tussocks exhibited increases in specific growth rates following clipping in mid-May. When foliage was removed from the upper portion of the canopy (younger foliage), regrowth rates and season-long aboveground biomass production were less than if the same amount of foliage was removed from low in the canopy (older foliage). The spatial pattern of defoliation also influenced tussock regrowth in a late-May clipping experiment, but differences in the effects of the clipping patterns were associated with the removal of apical meristems rather than with the age or location of foliage removed. Changes in tussock carbon dioxide and water vapor exchange that were associated with changes in growth following mid-May clipping were explored. All clipped tussocks showed increases in integrated daytime carbon dioxide uptake per unit foliage area after defoliation. Differences among treatments in the response of net daytime carbon gain during the first 24 hours after clipping corresponded well with differences in tussock regrowth over a 14-day period following clipping. Increased carbon gain of clipped tussocks was associated with increases in tussock water vapor conductance and intercellular carbon dioxide concentration, and decreases in the ratio of carbon dioxide uptake to water vapor loss. Differences among treatments in daytime carbon gain and regrowth were paralleled by the response of instantaneous rates of light-saturated net photosynthesis for entire tussocks. Defoliation increased the proportion of foliage directly illuminated within the tussock at solar noon. Changes in the fraction of sunlit foliage and the relative amounts of different-aged foliage in tussock canopies were correlated with the responses of light-saturated photosynthesis. Thus, the effects of the spatial pattern of foliage removal on canopy light microclimate and the age of remaining foliage had important implications for carbon gain and regrowth of tussocks following mid-May defoliation

Roles of replication fork-interacting and Chk1-activating domains from claspin in a DNA replication checkpoint response

Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. Claspin associates with replication forks upon origin unwinding. We show that Claspin contains a replication fork-interacting domain (RFID, residues 265–605) that associates with Cdc45, DNA polymerase ε, replication protein A, and two replication factor C complexes on chromatin. The RFID contains two basic patches (BP1 and BP2) at amino acids 265–331 and 470–600, respectively. Deletion of either BP1 or BP2 compromises optimal binding of Claspin to chromatin. Absence of BP1 has no effect on the ability of Claspin to mediate activation of Chk1. By contrast, removal of BP2 causes a large reduction in the Chk1-activating potency of Claspin. We also find that Claspin contains a small Chk1-activating domain (residues 776–905) that does not bind stably to chromatin, but it is fully effective at high concentrations for mediating activation of Chk1. These results indicate that stable retention of Claspin on chromatin is not necessary for activation of Chk1. Instead, our findings suggest that only transient interaction of Claspin with replication forks potentiates its Chk1-activating function. Another implication of this work is that stable binding of Claspin to chromatin may play a role in other functions besides the activation of Chk1