Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries

A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic evolution of ligands by exponential enrichment (SELEX). This overview unit discusses nucleic acid libraries that can be used, affinity probability distributions, an equilibrium model for SELEX, and optimal conditions including concentrations and signalâ toâ noise ratios.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143632/1/cpnc0901.pd

Using the Porter Model to analyze the U.S. elderberry industry

Paper presented at the 12th North American Agroforesty Conference, which was held June 4-9, 2011 in Athens, Georgia.In Ashton, S. F., S.W. Workman, W.G. Hubbard and D.J. Moorhead, eds. Agroforestry: A Profitable Land Use. Proceedings, 12th North American Agroforestry Conference, Athens, GA, June 4-9, 2011.Elderberry, a perennial shrub native to North America with a variety of uses and benefits, is neither well known nor widely utilized as a specialty crop in the U.S. Up-to-date information is lacking with regard to the elderberry market or market potential. This research identifies the market participants along the value chain, the current status of the industry, direction, and future trends, elderberry market limitations as well as risks and potential opportunities for elderberry producers and processors. A combination of quantitative (mail survey) and qualitative (phone interview) methods have been used. The theoretical model used for the survey and interview development and analysis is based on the Porter Five Forces Model (PFFM) which describes the competitive forces that coordinate and control the market. The PFFM has been used previously to shed light on the chestnut and shiitake mushroom specialty crop markets. Seventy-four mail survey responses and 20 follow up phone interviews provided information on the market participants, challenges, opportunities and competitive forces in the elderberry industry. Results show a nascent industry with mostly small scale participants poised for growth. Demand trends are favorable and prices are good across the value chain. Challenges include a limited domestic supply of fruit, few regionally adapted varieties suitable for commercial production, and high labor costs. Additionally, the absence of existing mechanical harvesting equipment limits future production potential and industry growth. Respondents identified low levels of competition within the industry at the present time. Based on identified market size and demand, opportunities exist to increase the domestic elderberry industry across the value chain.Mihaela M. Cernusca, Michael A. Gold and Larry D. Godsey ; The Center for Agroforestry, University of Missouri.Includes bibliographical references

From aptamer-based biomarker discovery to diagnostic and clinical applications: an aptamer-based, streamlined multiplex proteomic assay

Recently, we reported an aptamer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community

Growing and Marketing Elderberries in Missouri (2012)

The American elderberry (Sambucus canadensis, also known as Sambucus nigra subsp. canadensis) is native to much of eastern and midwestern North America. The plant is a medium to large multiple-stemmed shrub, bush or small tree. Elderberry is commonly found growing in a range of habitats throughout Missouri, but it prefers moist, well-drained, sunny sites and is often found along roadside ditches and streams.By Patrick L. Byers, Andrew L. Thomas, Mihaela M. Cernusca, Larry D. Godsey and Michael A. Gold (University of Missouri)Includes bibliographical reference

The Ursinus Weekly, March 25, 1971

Pettit reconsiders open dorm issue; USGA sets seven • Crime on campus continues; Checks, calculators copped • State of our prisons: Prison wardens defend merits of penal system • Editorial: Communications gap • Focus: Daphne Kline • Letters to the editor: Flak on Fred Flott; Haircut scholarships; Borish letter; Do-nothing air-heads; Sacred dormitories; Pettit praised • Movie critic: Friday night movies • Bloody good show • Grapplers finish winners; Ursinus\u27 big ten sighted • Delaware hosts female hoopmen • Tennis squad set; Jacob seeded 1st • Schaal honored; Named all starhttps://digitalcommons.ursinus.edu/weekly/1142/thumbnail.jp