Effects of Vanadium Pentoxide on the Histological and Sperm Parameters of Male Guinea Pigs

The pharmacological effects of intraperitoneal administration of different doses of vanadium pentoxide (V2O5) on the histological and sperm parameters of male guinea pigs were investigated. Also investigated were the effects of oral pretreatment with different doses of vitamin E (a known protein kinase C inhibitor) on the V2O5 -induced responses of the testis and liver of male guinea pigs. In n = 5 experiments, vanadium pentoxide in the dose range of 4.5-12.5mg/kg caused destruction of the testicular and liver architecture. This was characterized by a reduction in spermatogonia, destruction of seminiferous tubules, necrosis of the testicular tissues, necrosis of liver cells, fatty cells infiltration and vacoulation. Oral administration/ pretreatment with vitamin E in the dose range of 500-2000I.U caused a reversal of the vanadium pentoxide \u2013induced histological damages of the testis and the liver cells. Furthermore, in n = 5 animals experiments, Vanadium pentoxide (4.5-12.5 mg/kg/) caused a statistically significant increase in the percentage basal cell death, from 5.0 to 75.0 \ub1 1.0%, reduction in sperm motility from 90.0 to 31.0 \ub1 3.9%, reduction in sperm count from 80.0 x106cells/ml to 25.0\ub1 4.0 x 106cells/ml and alteration in the spermatic cell morphology ( i.e. causing a change in the cellular structure of sperm cells and an increase in abnormal cells count) of the male guinea pigs. These inhibitory effects were significant at P < 0.05 (ANOVA). These effects were all dose- and time-dependent and may have a role in oxidative pathology of vanadium pentoxide

Histopathological characteristics of female breast carcinomas seen at the University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria

No Abstract. Nigerian Journal of Medicine Vol. 14(1) 2005: 72-7

Abdominal actinomycosis, an unusual cause of intestinal obstruction

Background: Actinomycosis is a rare inflammatory disease caused by an anaerobic bacterium, Actinomyces israelii. Aim: To report a case of abdominal actinomycosis presenting as intestinal obstruction. Setting: University of Port Harcourt Teaching Hospital, Port Harcourt. Case report: A 54-year-old gentleman was involved in a road traffic accident in which he sustained fractures of the right humerus and pubic rami. A month later, he developed signs and symptoms of intestinal obstruction. At operation, a retroperitoneal mass obstructing the transverse colon and the proximal ileum was found and resected. Histological examination confirmed the mass to be due to actinomycosis infection. The patient was therefore placed on a parenteral therapy of 20 mega units of crystalline penicillin daily for three weeks and then a maintenance therapy of oral Amoxycillin for another six months. He recovered fully from the infection. Conclusion: Abdominal actinomycosis infection is an uncommon disease entity. Careful and expert histopathological analysis is essential in post operative diagnosis. Port Harcourt Medical Journal Vol. 1(1) September 2006: 65-6

Effects of lindane pretreatment on paraquat toxicity in female wistar rats (Rattus rattus)

No Abstract Supplie

Effects of Vanadium Pentoxide on the Histological and Sperm Parameters of Male Guinea Pigs

The pharmacological effects of intraperitoneal administration of different doses of vanadium pentoxide (V2O5) on the histological and sperm parameters of male guinea pigs were investigated. Also investigated were the effects of oral pretreatment with different doses of vitamin E (a known protein kinase C inhibitor) on the V2O5 -induced responses of the testis and liver of male guinea pigs. In n = 5 experiments, vanadium pentoxide in the dose range of 4.5-12.5mg/kg caused destruction of the testicular and liver architecture. This was characterized by a reduction in spermatogonia, destruction of seminiferous tubules, necrosis of the testicular tissues, necrosis of liver cells, fatty cells infiltration and vacoulation. Oral administration/ pretreatment with vitamin E in the dose range of 500-2000I.U caused a reversal of the vanadium pentoxide –induced histological damages of the testis and the liver cells. Furthermore, in n = 5 animals experiments, Vanadium pentoxide (4.5-12.5 mg/kg/) caused a statistically significant increase in the percentage basal cell death, from 5.0 to 75.0 ± 1.0%, reduction in sperm motility from 90.0 to 31.0 ± 3.9%, reduction in sperm count from 80.0 x106cells/ml to 25.0± 4.0 x 106cells/ml and alteration in the spermatic cell morphology ( i.e. causing a change in the cellular structure of sperm cells and an increase in abnormal cells count) of the male guinea pigs. These inhibitory effects were significant at P < 0.05 (ANOVA). These effects were all dose- and time-dependent and may have a role in oxidative pathology of vanadium pentoxide

Effects of Vanadium Pentoxide on the Histological and Sperm Parameters of Male Guinea Pigs

The pharmacological effects of intraperitoneal administration of different doses of vanadium pentoxide (V2O5) on the histological and sperm parameters of male guinea pigs were investigated. Also investigated were the effects of oral pretreatment with different doses of vitamin E (a known protein kinase C inhibitor) on the V2O5 -induced responses of the testis and liver of male guinea pigs. In n = 5 experiments, vanadium pentoxide in the dose range of 4.5-12.5mg/kg caused destruction of the testicular and liver architecture. This was characterized by a reduction in spermatogonia, destruction of seminiferous tubules, necrosis of the testicular tissues, necrosis of liver cells, fatty cells infiltration and vacoulation. Oral administration/ pretreatment with vitamin E in the dose range of 500-2000I.U caused a reversal of the vanadium pentoxide –induced histological damages of the testis and the liver cells. Furthermore, in n = 5 animals experiments, Vanadium pentoxide (4.5-12.5 mg/kg/) caused a statistically significant increase in the percentage basal cell death, from 5.0 to 75.0 ± 1.0%, reduction in sperm motility from 90.0 to 31.0 ± 3.9%, reduction in sperm count from 80.0 x106cells/ml to 25.0± 4.0 x 106cells/ml and alteration in the spermatic cell morphology ( i.e. causing a change in the cellular structure of sperm cells and an increase in abnormal cells count) of the male guinea pigs. These inhibitory effects were significant at P < 0.05 (ANOVA). These effects were all dose- and time-dependent and may have a role in oxidative pathology of vanadium pentoxide

Fibroadenoma cowxisting with infiltrating ductal carcinoma a case report

No Abstract. Nigerian Journal of Medicine Vol. 14(2) 2005: 221-22

Ocimum gratissimum Linn. Reverses cadmium-induced toxicity of spermatic parameters of the male guinea-pig

The influence of the aqueous crude extracts of Ocimum gratissimum Linn. leaf on cadmium (Cd)-induced toxic effects on spermatic parameters of the male guinea-pig (GP) was investigated. In n=5, Cd (0-8mg/kg) caused a dose-dependent inhibition or reduction of various spermatogenic parameters namely-number of normal sperm cells: 55.75±2.02 ×106 to 7.50±1.19 ×106/ml; number of abnormal sperm cells: 2.25±0.25 ×106 to 8.25±2.18 ×106/ml and total sperm count: 58.00±1.96 ×106 to 15.75±2.63 × 106/ml; motility: 64.25±2.39 % to 26.50±1.71%; morphology: 5.75±0.75 % to 38.25±2.72 %; and a significant increase (P less than 0.05) in particulate and primordial sperm cell counts in the male GP. However, injection of Ocimum gratissimum Linn. extract after Cd administration had little or no significant effect on the above mentioned parameters. Pre-treatment with 5mg of O. gratissimum, with subsequent administration of cadmium, blocked or reversed the Cd-induced toxicities on the various spermatogenic parameters- motility: 26.50±1.71% to 53.25±2.14%; morphology: 38.25±2.72% to 8.75±1.25%; number of normal sperm cells: 7.50±1.19 ×106 to 27.25±1.60 ×106/ml; number of abnormal sperm cells: 8.25±2.18 ×106 to 5.25±0.63 x106/ml and total sperm count: 15.75±2.63×106 to 32.50±1.85 ×106/ml. The observed influence of O. gratissimum on Cd-induced toxicity may be the consequence of the antioxidant action of the plant extract on the spermatogenic apparatus of the organism

The effects of ammonium metavanadate on biochemical hormonal, haematological and histopathological parameters of the female wistar rats

The effects of different doses of Ammonium metavanadate on the biochemical, haematological, hormonal and histopathological parameters of stilbesterol treated female Wistar rats were investigated. Ammonium metavanadate in the dose-range 0-6mg/kg caused a bi-phasic and time-dependent response on the acid (total and prostate) phosphatase.. Furthermore ammonium metavanadate caused a dose-dependent inhibition of the serum alkaline phosphatases. The maximal inhibitory response at 5mg/kg of ammonium metavanadate was 40.0 ±1.69 compared to 65.0 ±0.94 control values. Ammonium metavanadate also caused a positively correlated biphasic response in the serum female hormonal concentrations with an initial increase, followed by a time-dependent decrease in the serum values of luteinizing (LH), follicle stimulating hormone (FSH), prolactin . Furthermore ammonium metavanadate also caused time-and dose-dependent effects on the haematological parameters. The effects were biphasic-increase within 72 hours and a reduction in the values of haemoglobin and packed cell volume within 7-28 days. The white blood count and lymphocyte counts were also reduced significantly at P≤0.05. However the neutrophil counts were increased dose-and time-dependently. Finally, ammonium metavanadate caused a dose-dependent destruction of the liver and female reproductive organs namely the uterus, ovary and fallopian tubes. These were characterized by necrosis, oedema, eosinophilic deposits and vacuolation. These results may be explained by the oxidative effects caused by the free oxygen (O2 ) radical generated by the metavanadate ions

The effects of ammonium metavanadate on biochemical hormonal, haematological and histopathological parameters of the female Wistar rats

The effects of different doses of Ammonium metavanadate on the biochemical, haematological, hormonal and histopathological parameters of stilbesterol treated female Wistar rats were investigated. Ammonium metavanadate in the dose-range 0-6mg/kg caused a bi-phasic and time-dependent response on the acid (total and prostate) phosphatase.. Furthermore ammonium metavanadate caused a dose-dependent inhibition of the serum alkaline phosphatases. The maximal inhibitory response at 5mg/kg of ammonium metavanadate was 40.0 ±1.69 compared to 65.0 ±0.94 control values. Ammonium metavanadate also caused a positively correlated biphasic response in the serum female hormonal concentrations with an initial increase, followed by a time-dependent decrease in the serum values of luteinizing (LH), follicle stimulating hormone (FSH), prolactin . Furthermore ammonium metavanadate also caused time-and dose-dependent effects on the haematological parameters. The effects were biphasic-increase within 72 hours and a reduction in the values of haemoglobin and packed cell volume within 7-28 days. The white blood count and lymphocyte counts were also reduced significantly at P≤0.05. However the neutrophil counts were increased dose-and time-dependently. Finally, ammonium metavanadate caused a dose-dependent destruction of the liver and female reproductive organs namely the uterus, ovary and fallopian tubes. These were characterized by necrosis, oedema, eosinophilic deposits and vacuolation. These results may be explained by the oxidative effects caused by the free oxygen (O2 ) radical generated by the metavanadate ions