5,711 research outputs found
Recommended from our members
Targeted gene disruption of the endogenous c-abl locus by homologous recombination with DNA encoding a selectable fusion protein
We have introduced a substitution mutation into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene. Model constructs were initially generated that consisted of a promoterless selectable neomycin resistance marker inserted into the v-abl gene of the complete Abelson murine leukemia virus genome, designed to be expressed either as a fusion protein or by translational restart. Tests of these viral genomes for transmission of v-abl and neo markers showed more stable coexpression in a protein fusion construct. The neo fusion was subcloned from this v-abl construct into a promoterless c-abl fragment, and the resulting DNA was used to transform embryonic stem cells. Direct screening of genomic DNAs showed that a high proportion of drug-resistant clones arose from homologous recombination into the endogenous c-abl locus
Diffusive counter dispersion of mass in bubbly media
We consider a liquid bearing gas bubbles in a porous medium. When gas bubbles
are immovably trapped in a porous matrix by surface-tension forces, the
dominant mechanism of transfer of gas mass becomes the diffusion of gas
molecules through the liquid. Essentially, the gas solution is in local
thermodynamic equilibrium with vapor phase all over the system, i.e., the
solute concentration equals the solubility. When temperature and/or pressure
gradients are applied, diffusion fluxes appear and these fluxes are faithfully
determined by the temperature and pressure fields, not by the local solute
concentration, which is enslaved by the former. We derive the equations
governing such systems, accounting for thermodiffusion and gravitational
segregation effects which are shown not to be neglected for geological
systems---marine sediments, terrestrial aquifers, etc. The results are applied
for the treatment of non-high-pressure systems and real geological systems
bearing methane or carbon dioxide, where we find a potential possibility of the
formation of gaseous horizons deep below a porous medium surface. The reported
effects are of particular importance for natural methane hydrate deposits and
the problem of burial of industrial production of carbon dioxide in deep
aquifers.Comment: 10 pages, 5 figures, 1 table, Physical Review
Mars oxygen production system design
The design and construction phase is summarized of the Mars oxygen demonstration project. The basic hardware required to produce oxygen from simulated Mars atmosphere was assembled and tested. Some design problems still remain with the sample collection and storage system. In addition, design and development of computer compatible data acquisition and control instrumentation is ongoing
Development of a pilot data management infrastructure for biomedical researchers at University of Manchester – approach, findings, challenges and outlook of the MaDAM Project
Management and curation of digital data has been becoming ever more important in a higher education and research environment characterised by large and complex data, demand for more interdisciplinary and collaborative work, extended funder requirements and use of e-infrastructures to facilitate new research methods and paradigms. This paper presents the approach, technical infrastructure, findings, challenges and outlook (including future development within the successor project, MiSS) of the ‘MaDAM: Pilot data management infrastructure for biomedical researchers at University of Manchester’ project funded under the infrastructure strand of the JISC Managing Research Data (JISCMRD) programme. MaDAM developed a pilot research data management solution at the University of Manchester based on biomedical researchers’ requirements, which includes technical and governance components with the flexibility to meet future needs across multiple research groups and disciplines
Off-diagonal Interactions, Hund's Rules and Pair-binding in Hubbard Molecules
We have studied the effect of including nearest-neighbor, electron-electron
interactions, in particular the off-diagonal (non density-density) terms, on
the spectra of truncated tetrahedral and icosahedral ``Hubbard molecules,''
focusing on the relevance of these systems to the physics of doped C.
Our perturbation theoretic and exact diagonalization results agree with
previous work in that the density-density term suppresses pair-binding.
However, we find that for the parameter values of interest for the
off-diagonal terms {\em enhance} pair-binding, though not enough to offset the
suppression due to the density-density term. We also find that the critical
interaction strengths for the Hund's rules violating level crossings in
C, C and C are quite insensitive to the
inclusion of these additional interactions.Comment: 20p + 5figs, Revtex 3.0, UIUC preprint P-94-10-08
The phase diagram of the lattice Calogero-Sutherland model
We introduce a {\it lattice} version of the Calogero Sutherland model adapted
to describe pairwise interacting steps with discrete positions on a
vicinal surface. The configurational free energy is obtained within a transfer
matrix method. The full phase diagram for attractive and for repulsive
interaction is deduced. For attraction, critical temperatures of faceting
transitions are found to depend on step density.Comment: latex PRBCalogSuth.tex, 6 files, 4 pages [SPEC-S00/900
- …