PHYTOCHEMICAL COMPOSITIONS AND ORGAN WEIGHT EFFECTS OF MUCUNA SLOANEI (FABACEAE) IN ALBINO RATS (RATTUS NOVERGICUS)

This research was done to assess the phytochemical composition and possible effects for 28 days oral administration of aqueous crude seed extracts of Mucuna sloanei on organ weights of normal albino rats at weekly intervals. Forty eight adult male albino rats weighing between 150 to 250 g were divided into three treatment groups and one control group of twelve rats each. Each group consists of three replicates of four rats per replicate. The treatment groups were administered orally, 100 mg/kg, 200 mg/kg and 400 mg/kg of aqueous extracts of M. sloanei seed respectively. The results of the phytochemical screening of the aqueous extract showed that crude extracts of M. sloanei seed has flavonoids composition of (5.500±0.115 ), cyanide (0.054±0.001), alkaloids (1.000±0.058), tannins (0.540±0.006), phytates (14.50±0.058), saponins (6.100±0.058) and phenols (1.800±0.068) thus, having phytates as the most abundant and cyanide the least. There was no overall dose dependent and significant difference (p>0.05) observed in the organ weights in all the weeks when compared with the control, except the spleen, which showed a significant decrease (P<0.05) at 100 mg/kg at week one. Also, there was no significant difference (p>0.05) in the duration of treatments in the organ weights of kidney and lungs, but, a significant increase (p<0.05) was observed in the liver at week 4 when compared with week 1 and 2 at dose levels of 100 mg/kg. Similarly, there was an observed significant decrease (P<0.05) in the heart and spleen in 100 mg/kg and 400 mg/kg treated rats respectively when compared with the control. In conclusion, the aqueous extracts of the plant seems to have antimalarial, antioxidant, antihypertensive, hypocholesterolmic and anticarcinogenic effects owing to presence of such phytochemicals as alkaloids, tannins, flavonoids, phytate, phenol and saponins. It has also shown that the plant extract did not cause inflammation or constriction at the cellular level of the organs. Thus, the M. sloanei seed is good for consumption.Key words: Phytochemical composition, Organ weight, Aqueous extract, Mucuna sloanei, Albino rat

Human Immunodeficiency Virus Self‑Testing: Perspectives from Primary Healthcare Workers in Enugu State, Southeast Nigeria

Background: Stigmatization remains an intractable issue surrounding human immunodeficiency virus (HIV) management. Testing services are the gateway to treatment and prevention. HIV‑self testing (HIVST) is a panacea to this stigmatization. It is a simple friendly testing technique. This study assessed issues surrounding the HIVST from the perspectives of the primary health‑care workers in Enugu State. Methods: A cross‑sectional study was carried out using a semi‑structured interviewer‑administered questionnaire. A convenience sampling of 238 respondents were sampled from the 17 local government areas of the state. Analyses were performed using IBM‑SPSS version 23. Ethical approval was obtained from the Enugu State Ministry of Health. Results: The majority of the respondents were within the age of 31–50 years 84 (35.3%), predominantly females 203 (85.3%) and mainly married 191 (80.3%). Half of them had practiced for over 10 years and 145 (60.9%) had good knowledge of HIV screening. However, 23.9% understood HIVST as a  testing modality approved by the World Health Organization. A handful of them, 25 (10.5%) correctly identified the 5Cs of HIV testing. Only 162 (68.1%) of the health‑care workers reported stigma as the most perceived problems of the present testing modality and 146 (61.3%) expressed a  preference for HIVST over previous methods. Age was found to be a determinant of good knowledge of HIVST, as being within the age group of  20–40 years has a 1.83 greater odds of good knowledge of HIVST than, 41–60 years age group (Adjusted odds ratios = 1.830; 95% confidence interval  1.081–3.099). Conclusion: Most of the Primary health‑care workers in Enugu State had poor knowledge of HIVST. These workers could benefit from awareness creation and training on HIVST by public health specialists to improve their knowledge. Keywords: Enugu state, human immunodeficiency virus self‑testing, perspectives, primary health‑care worker

Effect of Vernonia amygdalina Ethanolic Root Extract on the Hepato- and Nephro-Protective Properties of Albino Rats (Rattus novergicus)

The hepato- and nephro-protective potentials of Vernonia amygdalina ethanolic root extract was evaluated for 14 days using standard bioassay in 45 normal male albino rats. The rats were divided into four treatment groups I – IV and a control group V. Groups I – IV were given 100mg.kg-1, 200mg.kg-1, 400mg.kg-1 and 600mg.kg-1 body weight, respectively while the control (group V) was given equal volume of feed and water. The extracts were administered orally to the animals for 14 days. Blood samples were collected using the ocular puncture method before and weekly after administration to evaluate the extracts’ effects on aspartate transaminase (AST), acid phosphatase (ACP), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin, creatinine and blood urea nitrogen (BUN) levels. The mean serum levels of the liver marker enzymes AST, ACP, ALT, ALP, total bilirubin and direct bilirubin ranged from 10.00±0.53 to 11.44±0.44, 31.29±0.64 to 33.14±0.56, 27.22±0.94 to 29.67±0.37 and 37.83±0.59 to 40.57±1.02, 3.42±0.08 to 3.61±0.07 and 2.06±0.11 to 2.51±0.05 respectively. The mean levels of the nephrotic enzymes, creatinine and BUN also ranged from 39.87±1.79 to 43.04±1.57 and 6.62±0.21 to 15.98±0.17 accordingly. Although no significant difference (p>0.05) was observed in the serum levels of the liver marker enzymes and creatinine when compared with the control, a dose and duration dependent significant increase (p<0.05) occurred in the BUN level. This tends to suggest that the ethanolic root extract of Vernonia amygdalina on a short term basis has some hepato-protective property while its nephro-protective ability is still doubtful. Keywords: Vernonia amygdalina, Ethanolic root extract, Liver maker enzymes, Nephrotic enzymes, Albino rat

Impact of Mobile Technologies on Cervical Cancer Screening Practices in Lagos, Nigeria (mHealth-Cervix): A Randomized Controlled Trial

Purpose We assessed the impact of mHealth on Pap test screening uptake and also determined the factors that affect screening uptake among women in Lagos, Nigeria. Materials and Methods A randomized controlled trial was carried out among women in two tertiary health institutions in Lagos, Nigeria, between July 2020 and March 2021. Participants were randomly assigned to either a text message (mHealth) intervention or usual care arm. The main study outcome was the uptake of Pap smear testing within 6 months of enrollment in the study. We tested the associations between two groups of continuous variables using the unpooled independent-sample t-test (normal distribution) and that of two groups of categorical variables with the chi-square (χ2) test. Using a multinomial logistic regression model, we adjusted for relevant sociodemographic and clinical predictors of uptake of Pap smear screening. Statistical significance was defined as P < .05. Results There was a significantly higher rate of uptake of Pap smear screening among women in the mHealth arm compared with those in the usual care arm (51.0% v 35.7%, P = .031). Following adjustment in the final multivariate model, level of income (odds ratio [OR] = 5.13, 95% CI, 1.55 to 16.95), awareness of Pap smear (OR = 16.26; 95% CI, 2.49 to 76.64), General Outpatient clinic attendance, and introduction of mHealth intervention during follow-up (OR = 4.36; 95% CI, 1.44 to 13.22) were the independent predictors of Pap smear uptake. Conclusion The use of mHealth technologies intervention via short-text message services is a feasible solution for cervical cancer prevention in low- and middle-income countries, and thus, the widespread use of mHealth services by health care providers and policymakers could contribute to the implementation of cervical cancer prevention services in Nigeria and in the settings of other low- and middle-income countries

Comparative phytochemical and antioxidant profile of crude seed powder, aqueous and methanolic seed extracts of Buchholzia coriacea

The increasing discovery of more medicinal plants have triggered increased scientific screening of their bioactivity in order to provide data that will help physicians and patients make wise decision before using them. This study was designed to elucidate comparative phytochemical and antioxidant properties of crude seed powder, aqueous and methanolic seed extracts of Buchholzia coriacea. The results showed that crude seed powder had the highest alkaloid and phenol content of 3.98 ± 0.00% and 0.92 ± 0.00%, while aqueous extract had the least alkaloid and phenol content of 1.00 ± 0.00% and 0.12 ± 0.00% respectively. Methanolic extracts had the highest phytochemical components among the three extracts with flavonoids (12.03 ± 0.0), saponins (1.99 ± 0.01), terpenoids (2.00 ± 0.00), tannin (0.10 ± 0.00) and phytate (2.02 ± 0.01) compositions, while aqueous extracts had the highest hydrogen cyanide (0.30 ± 0.00) and glycoside (0.35 ± 0.00). Antioxidant (DPPH) activities of B. coriacea showed that aqueous extract and crude seed powder had inhibition concentration (IC50) of 4.65 mg/ml while methanolic extract had IC50 of 5.85 mg/ml. The result of the LD50 of the extracts showed the each extracts was well tolerated at a dose of 5000 mg/kg, an indication of high safety profile. The study therefore clearly demonstrated that methanolic extracts of B. coriacea have antioxidant, antihypertensive, hypocholesterolmic and anticarcinogenic properties owing to the presence of high levels of phytochemical components than the aqueous and crude seed powder.

Eco-physiological adaptation of the land snail Achatina achatina (Gastropoda: Pulmonata) in tropical agro-ecosystem

The survival of land snails in an adverse environmental condition depends on the integral physiological, morphological and behavioural adaptations. These adaptations are essential in understanding the species-specific habitat requirements and in predicting their environmental responses. In this study, the monthly and the periodic patterns of eco-physiological adaptation of land snail, Achatina achatina in Nsukka tropical agro-ecosystem were assessed from December 2012 to July 2013. Standard methods were employed in sampling the land snail and determination of the water content, biochemical fuel reserves and enzyme concentrations of the samples. The present results showed that lipids were high at the beginning of aestivation and depleted as the aestivation progressed. Glycogen was significantly low throughout the aestivation months (December–March) and increased in the active months (April–July). Protein content recorded a definite pattern all through the months studied. Catabolism of lactate and a decrease in activity of LDH during aestivation and substantial increase upon activation were observed. Data showed that transaminase and aspartate enzymes depleted during the aestivation months indicating that the snails may have developed potential cell injury due to oxidative stress and thermal heat. A disassociation between the physiological responses and climatic data was recorded. The physiological adaptation of A. achatina ensures regular adjustment under extreme conditions and compensates for its metabolic regulation in the tropics. It is concluded that survival of A. achatina is not environmentally predicted; rather it depends on the species-specific inherent process in predicting responses for survival

Adaptation of the Wound Healing Questionnaire universal-reporter outcome measure for use in global surgery trials (TALON-1 study): mixed-methods study and Rasch analysis

BackgroundThe Bluebelle Wound Healing Questionnaire (WHQ) is a universal-reporter outcome measure developed in the UK for remote detection of surgical-site infection after abdominal surgery. This study aimed to explore cross-cultural equivalence, acceptability, and content validity of the WHQ for use across low- and middle-income countries, and to make recommendations for its adaptation.MethodsThis was a mixed-methods study within a trial (SWAT) embedded in an international randomized trial, conducted according to best practice guidelines, and co-produced with community and patient partners (TALON-1). Structured interviews and focus groups were used to gather data regarding cross-cultural, cross-contextual equivalence of the individual items and scale, and conduct a translatability assessment. Translation was completed into five languages in accordance with Mapi recommendations. Next, data from a prospective cohort (SWAT) were interpreted using Rasch analysis to explore scaling and measurement properties of the WHQ. Finally, qualitative and quantitative data were triangulated using a modified, exploratory, instrumental design model.ResultsIn the qualitative phase, 10 structured interviews and six focus groups took place with a total of 47 investigators across six countries. Themes related to comprehension, response mapping, retrieval, and judgement were identified with rich cross-cultural insights. In the quantitative phase, an exploratory Rasch model was fitted to data from 537 patients (369 excluding extremes). Owing to the number of extreme (floor) values, the overall level of power was low. The single WHQ scale satisfied tests of unidimensionality, indicating validity of the ordinal total WHQ score. There was significant overall model misfit of five items (5, 9, 14, 15, 16) and local dependency in 11 item pairs. The person separation index was estimated as 0.48 suggesting weak discrimination between classes, whereas Cronbach's α was high at 0.86. Triangulation of qualitative data with the Rasch analysis supported recommendations for cross-cultural adaptation of the WHQ items 1 (redness), 3 (clear fluid), 7 (deep wound opening), 10 (pain), 11 (fever), 15 (antibiotics), 16 (debridement), 18 (drainage), and 19 (reoperation). Changes to three item response categories (1, not at all; 2, a little; 3, a lot) were adopted for symptom items 1 to 10, and two categories (0, no; 1, yes) for item 11 (fever).ConclusionThis study made recommendations for cross-cultural adaptation of the WHQ for use in global surgical research and practice, using co-produced mixed-methods data from three continents. Translations are now available for implementation into remote wound assessment pathways