Partial replacement of dietary soybean meal by high‐protein distiller's dried grains (HPDDG) supplemented with protease enzyme for European seabass, Dicentrarchus labrax fingerlings

High‐protein distillers dried grains (HPDDG) is a co‐product of ethanol production that uses prefractionation technology. A 70‐day growth trial was conducted to investigate the effect of partial replacement of dietary soybean meal by high‐protein distiller's dried grains (HPDDG) with protease enzyme supplementation (PROXYM ULTRA®) on growth performance, physiological parameters and histological changes of the intestine of European sea bass, Dicentrarchus labrax fingerlings. The results indicated that increased dietary HPDDG levels up to 50% of HPDDG supplemented with protease significantly increases growth performance and feed utilization and improved FCR of sea bass. In addition, replacement of SBM by HPDDG supplemented with protease enhanced feed intake efficiency and the health status of fish. Haematology and serum biochemistry (haemoglobin (Hb)), red blood cells (RBCs), white blood cells (WBCs) and humeral immune parameters including total protein, globulin, cholesterol, lysozyme activity and total antioxidant capacity significantly increased with increase in HPDDG supplemented with protease in the diets. Results of this study indicated that HPDDG supplemented with protease is a good alternative protein source for aquaculture feed and can be included up to 50% as a replacement of SBM without compromising growth performance and physiological parameters of sea bass

Impact of nucleotide enriched diets on the production of gilthead seabream, Sparus aurata fingerlings by modulation of liver mitochondrial enzyme activitity, antioxidant status, immune gene expression, and gut microbial ecology

Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.[EN] The objective of this study was to evaluate the effect of fish meal replacement with a mixture of plant, and animal protein supplemented with a commercial nucleotide NucleofomeFish (TM), on growth, feed utilization, whole-body composition, biometric indices; liver mitochondrial enzyme activities, selected gene expression and influence on the gut micmbiome of gilthead seabream, Sparus aurata. Six hundred and thirty gilthead seabream with an average initial body weight of 0.358 +/- 0.002 g/fish, were used. Six experimental diets were formulated to be isonitrogenous, 45% crude protein (CP) and isocaloric 20 MJ/kg gross energy (GE). The control diet (Dl) contained 25% of fish meal (FM25) and had no NucleoforceFish (TM) added (FM25/0), diets 2-3 contained FM at a level of 25% and supplemented with 250 (FM25/250) and 500 (FM25/500) mg/kg NucleoforceFish (TM), respectively. Diets 4-6 were a non-fishmeal formulation and had no NucleoforceFish (TM), (diet 4, FM0/0), 0%FM with 250mg/kg NucleoforceFish (TM) (diet 5, FM0/250), and 0%FM with 500mg/kg NucleofomeFish (TM) (diet 6, FM0/500). The results revealed that dietary nucleotides supplemented at 250mg/kg or 500mg/kg enhanced final body weight, weight gain, and specific growth rate of seabream either with dietary level of 25% FM diet (FM25) or non-fishmeal diet (FM0). All mitochondrial liver complex enzyme activities were recorded as having a significant positive response to the nucleotide dietary supplement. Gilthead seabream fingerlings fed FM0/250 diet for 150 days had significantly elevated liver mitochondrial enzyme (Complex III, Complex IV, Complex I and II) activities. In addition, gilthead seabream fed FM(0/500 )had significantly increased the gene expression of TCR-B and IL1-B, while fish fed FM25/250 increased significantly the gene expression of IGF1, while fish fed FM(25/500 )increased significantly the gene expression of Hepcidin (Hep) and the iron homeostasis hormone. There was a marked influence on specific bacteria comprising the gut microbiome namely Photobacterium; damselae; Vibrio anguillarum; Lactobacillus plantarum; Lactobacillus Enterococcus faecium subtilis. It can be concluded that FM25/250 and FM25/500 diets are expressing the best result, which explains that FM must be in the feed of gilthead seabream, and addition of NucleoforceFish (TM) at 250 mg/kg feed may further increase the performance.El-Nokrashy, AM.; El-Banna, R.; Edrise, B.; Abdel-Rahim, M.; Jover Cerda, M.; Tomas-Vidal, A.; Prince, A.... (2021). Impact of nucleotide enriched diets on the production of gilthead seabream, Sparus aurata fingerlings by modulation of liver mitochondrial enzyme activitity, antioxidant status, immune gene expression, and gut microbial ecology. Aquaculture. 535:1-13. https://doi.org/10.1016/j.aquaculture.2021.736398S11353