37 research outputs found

    Meat protein aggregation: First investigations on the role of the lipid oxidation product, 4-hydroxy-2-nonenal

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    International audienceThis paper describes for the first time how 4-hydroxy-2-nonenal (HNE) promotes protein aggregation in meat products. A whole meat extract was incubated with increasing concentrations of HNE during 30min, 1h and 2h at 25°C. In the studied conditions, HNE-protein adducts were detected. In presence of HNE, protein solubility decreased that stressed a possible involvement of HNE in the aggregation process. Different analytical methods were investigated to detect aggregates formation. The aggregation phenomenon remained low. Employed methods give us information on the implicated bonds. Electrophoresis with or without using a reducing agent coupled with western-blotting analyses revealed that HNE attacks preferentially the cysteine residues (by thiols function) and then lysine and histidine

    Meat protein aggregation: First investigations on the role of the lipid oxidation product, 4-hydroxy-2-nonenal

    No full text
    International audienceThis paper describes for the first time how 4-hydroxy-2-nonenal (HNE) promotes protein aggregation in meat products. A whole meat extract was incubated with increasing concentrations of HNE during 30min, 1h and 2h at 25°C. In the studied conditions, HNE-protein adducts were detected. In presence of HNE, protein solubility decreased that stressed a possible involvement of HNE in the aggregation process. Different analytical methods were investigated to detect aggregates formation. The aggregation phenomenon remained low. Employed methods give us information on the implicated bonds. Electrophoresis with or without using a reducing agent coupled with western-blotting analyses revealed that HNE attacks preferentially the cysteine residues (by thiols function) and then lysine and histidine

    Matrix impact on the bioaccessibility of plant polyphenols in the gastric tract

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    The consumption of fruit and vegetables (F&V) is inversely associated to the development of cardiovascular diseases, several kinds of cancers and other chronic diseases. Moreover, the cardiovascular protection could be ascribed to flavonoids, a class of polyphenols largely distributed in F&V. Polyphenols are poorly absorbed from the gastrointestinal tract (GIT) and undergo extensive metabolization by colon microflora before reaching plasma. Thus, before their absorption, polyphenols could exert a health benefit directly in the GIT. As a matter of fact, they were shown to protect polyunsaturated lipids from oxidation during gastric digestion. The bioaccessibility of polyphenols, defined as their release from the food matrix and solubilization into the bolus, is a prerequisite step
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