5,076 research outputs found
Visualizing mitochondrial FoF1-ATP synthase as the target of the immunomodulatory drug Bz-423
Targeting the mitochondrial enzyme FoF1-ATP synthase and modulating its
catalytic activities with small molecules is a promising new approach for
treatment of autoimmune diseases. The immuno-modulatory compound Bz-423 is such
a drug that binds to subunit OSCP of the mitochondrial FoF1-ATP synthase and
induces apoptosis via increased reactive oxygen production in coupled, actively
respiring mitochondria. Here we review the experimental progress to reveal the
binding of Bz-423 to the mitochondrial target and discuss how subunit rotation
of FoF1-ATP synthase is affected by Bz-423. Briefly, we report how F\"orster
resonance energy transfer (FRET) can be employed to colocalize the enzyme and
the fluorescently tagged Bz-423 within the mitochondria of living cells with
nanometer resolution.Comment: 10 pages, 2 figure
RNA Folding Pathways
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143692/1/cpnc1100.pd
Going forward
No abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/55863/1/20632_ftp.pd
Fatty acid composition of Neurospora plasma membrane
Fatty acid composition of Neurospora plasma membran
Incorporation and degradation of lignoceric acid in cel
Incorporation and degradation of lignoceric acid in ce
36 degree step size of proton-driven c-ring rotation in FoF1-ATP synthase
Synthesis of the biological "energy currency molecule" adenosine triphosphate
ATP is accomplished by FoF1-ATP synthase. In the plasma membrane of Escherichia
coli, proton-driven rotation of a ring of 10 c subunits in the Fo motor powers
catalysis in the F1 motor. While F1 uses 120 degree stepping, Fo models predict
a step-by-step rotation of c subunits 36 degree at a time, which is here
demonstrated by single-molecule fluorescence resonance energy transfer.Comment: 8 pages, 1 figur
Engineering Disulfide CrossâLinks in RNA Via Air Oxidation
This unit presents protocols for the synthesis of alkylthiolâmodified ribonucleosides, their incorporation into synthetic RNA, and the formation of intramolecular disulfide bonds in RNA by air oxidation. The disulfide bonds can be formed in quantitative yields between thiols positioned in close proximity by virtue of either the secondary or tertiary structure of the RNA. Disulfide crossâlinks are useful tools to probe solution structures of RNA, to monitor dynamic motion, to stabilize folded RNAs, and to study the process of tertiary structure folding.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143806/1/cpnc0504.pd
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