Heme triggered-coagulation activation is tissue factor-dependent.

<p>A human inhibitory anti-tissue factor antibody (Ref 4509 Sekisui) significantly delayed the coagulation initiation, prolonging the clotting time (A) and the time to maximal velocity of clot formation (C). No change was observed in the clot formation time (B) or area under curve (D). Two tailed Wilcoxon test was performed and p-values < 0.05 were considered significant. CFT: clot formation time; MAXV-T: time of maximal velocity; AUC: area under curve; Anti-TF: Tissue factor inhibiting antibody.</p

Activation of coagulation and clot formation induced by heme.

<p>Comparisons were made for heme <i>vs</i> vehicle after 4h of incubation. Histograms were obtained for selected Rotem parameters and show mean (±SEM). Heme induced a significant reduction of clotting time (A), clot formation time (B) and time for maximal velocity (C). No significant change was observed for the area under the curve (D). Two tailed Wilcoxon test was performed and p-values < 0.05 were considered significant. CFT: clot formation time; MAXV-T: time of maximal velocity; AUC: area under curve.</p