Aspergillus section fumigati pneumonia and oxalate nephrosis in a foal

Equine pulmonary aspergillosis is a rare deep mycosis often due to the hematogenous spread of hyphae after gastrointestinal tract disease. We describe herein the main clinic-pathological findings observed in a foal, which spontaneously died after showing diarrhea and respiratory distress. Necropsy and histopathological investigations allowed to diagnose pulmonary aspergillosis, which likely developed after necrotic typhlitis-colitis. Biomolecular studies identified Aspergillus section Fumigati strain as the causative agent. Notably, severe oxalate nephrosis was concurrently observed. Occasionally, oxalate nephropathy can be a sequela of pulmonary aspergillosis in humans. The present case report suggests that the renal precipitation of oxalates can occur also in horses affected by pulmonary aspergillosis and could likely contribute to the fatal outcome of the disease

Risk of introduction of Clinostomum complanatum (Digenea: Clinostomidae) to Sicily through use of Cobitis bilineata (Canestrini, 1865) as live baits

Clinostomum complanatum (Rudolphi, 1814) is a digenetic trematode infecting fish-eating birds, reptiles and occasionally humans. Freshwater snails are the first intermediate hosts and many fish species and amphibians are second intermediate hosts. The aim of this study was to report Cobitis bilineata as a new intermediate host of C. complanatum. In this study, we found infections of C. complanatum metacercariae in 12 out of 30 (P= 40%) C. bilineata originating from northern Italy. Infected fish had 2 or 3 encysted metacercariae close to the gill operculum and lower jaw. Parasites were removed and identified both morphologically and molecularly using sequences of a mitochondrial gene, cytochrome c oxidase I, and the ribosomal markers. Some cysts extracted from dead fish were processed for histopathological investigations. The risk of the introduction of the parasitosis also in other new areas, such as Sicily, using live baits is discussed

Ingestion of plastic and non-plastic microfibers by farmed gilthead sea bream (Sparus aurata) and common carp (Cyprinus carpio) at different life stages

Environmental pollution by plastic particles is of major global concern, as a potential threat to aquatic organisms and ecosystems. The accumulation of microplastics in freshwater and marine environments has strong ecological implications due to their long persistence, potential toxicity, and ability to adsorb other pollutants, acting as vectors of pathogens. Nevertheless, while the number of studies on the presence of microplastics in the wild fish increased, less attention has been paid to the farmed fish species. Here, we investigated the occurrence of microparticles in the digestive tracts of Sparus aurata and Cyprinus carpio at different life stages and reared by an intensive and semi-intensive production system, respectively. Our results showed the presence of natural microfibers and microplastics, with microfibers (~ 90%) being the dominant type. In both fish species, the presence of microparticles was not revealed at larval stage. Fry and adult S. aurata specimens showed microfiber abundances of 0.21 and 1.3 items/individual, respectively. A lower load of microparticles (p < 0.05) occurred in fry (0.06 items/individual) and adult C. carpio specimens (0.25 items/individual). As to the chemical composition of the micro-items, natural (20%), semi-synthetic (28%), and single or blended synthetic fibers (52%) were identified in S. aurata. Linen, rayon, lyocell, cotton: polyester and polyester (12.5% concentration for each polymer) fibers were identified in C. carpio, while PTFE (37.5%) was present as fragments. Rayon was the most frequent chemical type (21.2%). The polymer composition of the extracted microparticles showed significant differences between the fish species analysed (p < 0.05). Notably, a considerably lower contamination level of synthetic polymers (average 0.11 items/individual) was detected in the farmed fishes compared with the data obtained in other studies on feral ones. To the best of our knowledge, this is the first study reporting the plastic and the non-plastic microfiber contamination in the farmed S. aurata and C. carpio at different life stages