Induction of ovarian maturation by means of dietary hormonal treatment in Austropotamobius pallipes

The freshwater crayfish Austropotamobius pallipes is an annual species with low fecundity and a long embryonic development. Restocking programmes for this species have recently been prompted in many countries in Europe because of its ecological importance in the freshwater ecosystem. The role and interactions of neurotransmitters which intervene in crustacean reproduction have been identified but they are not still completely understood. Ovarian development appears to be under the control of two hormones: the vitellogenesis-inhibiting hormone and the gonad stimulating hormone (Fingermann, 1997)

First occurrence of Callinectes sapidus (Rathbun, 1896) within the Sacca di Goro (Italy) and surroundings

The Sacca di Goro lagoon is an area located in the northern part of Italy. This locality has benefited in the past of an occasional and later the lanned introduction of the Manila clam. Nowadays it produces more than 50% of the entire Italian clam production. Recently the crab Callinectes sapidus has been spotted and reported a few months ago on its female carrying eggs. This may signify the complete acclimation of this species in the Sacca di Goro lagoon and a potential ongoing spread to surrounding areas

Reproductive plasticity of a Procambarus clarkii population living 10\ub0C below its thermal optimum

In this study the annual reproductive biology of a Procambarus clarkii (Girard, 1852) population living in an atypical habitat with cold spring waters is investigated by monitoring Gonado-Somatic and Hepato-Somatic Indexes (GSI and HSI) and by performing cytology on ovaries. Despite its known preference for habitats with water temperature from 21 to 30 \ub0C, our results clearly confirm the adaptation of this population to the atypical thermal habitat, characterised by an annual mean water temperature value of 13.32 \ub10.08 \ub0C. Maximum gonadal development was reached in August, with maximum GSI median value of 0.64 (instead of reported values even 10 times higher for other populations), and ovigerous females were found in autumn, with mean realized fecundity of 35 \ub17 compared to 285\u2013995 reported from other habitats. Histological analysis was consistent with other studies and allowed us to follow ovarian development at cytological level. The importance of all these results is not to be underestimated: to our knowledge these findings are the first report of the coolest habitat successfully colonized by this species at the present time and so they have to be taken as a warning about the possible range expansion of P. clarkii also to northern and colder habitats that have few things in common with the native habitat of the species and, up to now, were considered \u201csafe\u201d from the invasion of the red swamp crayfish

The eyestalk transcriptome of red swamp crayfish Procambarus clarkii

The red swamp crayfish (. Procambarus clarkii, Girard 1852) is among the most economically important freshwater crustacean species, and it is also considered one of the most aggressive invasive species worldwide. Despite its commercial importance and being one of the most studied crayfish species, its genomic and transcriptomic layout has only been partially studied. Illumina RNA-sequencing was applied to characterize the eyestalk transcriptome and identify its most characterizing genes. A collection of 83,170,732 reads from eyestalks was obtained using Illumina paired-end sequencing technology. A de novo assembly was performed with the Trinity assembly software generating 119,255 contigs (average length of 1007. bp) and identifying the first sequenced transcriptome in this species.The eyestalk is a major site for the production of neurohormones and controls a variety of physiological functions such as osmotic regulation, molting, epidermal color patterns and reproduction. Hence, its transcriptomic characterization is interesting and potentially instrumental to the elucidation of genes which have not been comprehensively described yet. Moreover, the availability of such a large amount of information supported the characterization of molecular families which have never been described before. The P. clarkii eyestalk transcriptome reported here provides a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species and represents an important source of data for all the interested carcinologists

Impact of agrochemicals on non-target species: Calathus fuscipes Goeze 1777 (Coleoptera: Carabidae) as model

Carabid beetles are important in the biological control of arable crop pests. Agricultural practices can produce over time a delayed toxic effect at the organismal and population levels and can compromise the survival on these species. In this research, we quantified the cumulative sublethal effect on body size, Malpighian tubules and immune responses in Calathus fuscipes adults living in the potato field and exposed to lambda-cyhalothrin and cymoxanil-based commercial formulates. Reductions of morphological parameters such as body, pronotum and elytron in both males and females from the potato field indicated that the pre-imaginal stages (larvae and pupae) suffer the sublethal effects of exposure to the larvicide control action of lambda-cyhalothrin. Ultrastructural alterations recorded in Malpighian tubules at the level of plasma membrane, mitochondria and nucleus indicated the reduction of the detoxification capability. The basal phenoloxidase and lysozyme-like enzyme activities have measured as markers of immune competence. Spectrophometric analyses showed that the chronic exposure in field causes an increase of basal phenoloxidase enzyme activity, while the lytic activity of haemolymph was not affected. As a result, the use of larvicides and fungicides have a harmful effect on beneficial species such C. fuscipes living in the soil of potato fields. These morphological and physiological results recorded at the organismal level can provide useful information of effects at the population and community levels to preserve the biodiversity of agroecosystem

Apis mellifera ligustica, Spinola 1806 as bioindicator for detecting environmental contamination: a preliminary study of heavy metal pollution in Trieste, Italy

Honeybees have become important tools for the ecotoxicological assessment of soil, water and air metal contamination due to their extraordinary capacity to bioaccumulate toxic metals from the environment. The level of heavy metal pollution in the Trieste city was monitored using foraging bees of Apis mellifera ligustica from hives owned by beekeepers in two sites strategically located in the suburban industrial area and urban ones chosen as control. The metal concentration in foraging bees was determined by inductively coupled plasma-mass spectrometry. The chemical analysis has identified and quantified 11 trace elements accumulated in two different rank orders: Zn> Cu > Sr > Bi > Ni > Cr > Pb = Co > V > Cd > As in foraging bees from the suburban site and Zn > Cu > Sr > Cr > Ni > Bi > Co = V > Pb > As > Cd in bees from urban site. Data revealed concentrations of Cr and Cu significantly higher and concentration of Cd significantly lower in bees from urban sites. The spatial difference and magnitude order in heavy metal accumulation along the urban-suburban gradient are mainly related to the different anthropogenic activity within sampled sites and represent a risk for the human health of people living in the city. We discussed and compared results with the range of values reported in literature

Biology and distribution of Danube barbel (Barbus balcanicus) (Osteichthyes: Cyprinidae) at the Northwestern limit of its range

Presence of Danube barbel Barbus balcanicus was investigated at the westernmost portion of its distribution area (Italian portion of the Isonzo River Basin). Genetic analyses carried out on specimens collected in different watercourses confirmed B. balcanicus presence in the same locations where Barbus caninus was erroneously classified in the past. More precisely, 4 Cyt b haplotypes belonging to the Balkanic species were described, three of which were evidenced for the first time. Analysis of meristic characters also confirmed genetic results and completed the taxonomical assessment. At the same time, a target population was studied to deepen knowledge about the species ecology. In this case, 120 specimens belonging to five age classes (from 0+ to 4+) were collected with monthly frequency in Barbucina Creek, representing the model watercourse inhabited by the species in the study area. Regression between total weight (W) and standard length (SL) did not differ significantly between males and females, therefore not displaying sexual dimorphism. SL values showed wide ranges, often overlapping among age classes. Mean condition factor (Kmean) decreased significantly with growth and age, as small individuals were in better nutritional condition. From an ecological point of view, biometric parameters seemed to be affected by habitat conditions and, specifically, to the limited space of the creeks, rather than by trophic conditions. Finally, trends of gonadosomatic index (GSI) showed that B. balcanicus reproductive period stretches between April and late May/early June. Results indicated that sexual maturity is reached at 1+ age, corresponding to a mean standard length equal to 5.50 ± 0.66 for female and 5.71 ± 0.53 for male breeders. In conclusion, experimental results highlighted the presence and adaptability of this "forgotten" species of the Italian fish fauna, which certainly will deserve attention in a future amendment of the Italian IUCN Red List of vertebrates

Gene Expression Profiling of Trematomus bernacchii in Response to Thermal and Stabling Stress

The Antarctic continent is one of the most pristine environments on planet Earth, yet one of the most fragile and susceptible to the effects of the ongoing climate change. The overwhelming majority of the components of Antarctic marine trophic chain are stenotherm organisms, highly adapted to the extreme, but extremely stable, freezing temperatures of the Antarctic ocean, which have not changed significantly during the past fifteen million years. Notothenioid fishes are the most abundant representatives of ichthyofauna at these latitudes, being ubiquitously found in coastal areas across the entire continent. While different Antarctic fish species have been previously subjected to studies aimed at defining their range of thermal tolerance, or at studying the response to acute thermal stress, just a handful of authors have investigated the effects of the exposure to a moderate increase of temperature, falling within the expected forecasts for the next few decades in some areas of the Antarctica. Here, the emerald rockcod Trematomus bernacchii was used as a model species to investigate the effects of a 20-day long exposure to a +1.5 °C increase in the brain, gills and skeletal muscle, using a RNA-sequencing approach. In parallel, the experimental design also allowed for assessing the impact of stabling (including acclimation, the handling of fishes and their confinement in tanks during the experimental phase) on gene expression profiling. The results of this study clearly identified the brain as the most susceptible tissue to heat stress, with evidence of a time-dependent response dominated by an alteration of immune response, protein synthesis and folding, and energy metabolism-related genes. While the gills displayed smaller but still significant alterations, the skeletal muscle was completely unaffected by the experimental conditions. The stabling conditions also had an important impact on gene expression profiles in the brain, suggesting the presence of significant alterations of the fish nervous system, possibly due to the confinement to tanks with limited water volume and of the restricted possibility of movement. Besides providing novel insights in the molecular mechanisms underlying thermal stress in notothenioids, these findings suggest that more attention should be dedicated to an improved design of the experiments carried out on Antarctic organism, due to their extreme susceptibility to the slightest environmental alterations

Identification and Characterization of a Novel Family of Cysteine-Rich Peptides (MgCRP-I) from Mytilus galloprovincialis

We report the identification of a novel gene family (named MgCRP-I) encoding short secreted cysteine-rich peptides in the Mediterranean mussel Mytilus galloprovincialis. These peptides display a highly conserved pre-pro region and a hypervariable mature peptide comprising six invariant cysteine residues arranged in three intramolecular disulfide bridges. Although their cysteine pattern is similar to cysteines-rich neurotoxic peptides of distantly related protostomes such as cone snails and arachnids, the different organization of the disulfide bridges observed in synthetic peptides and phylogenetic analyses revealed MgCRP-I as a novel protein family. Genome- and transcriptome-wide searches for orthologous sequences in other bivalve species indicated the unique presence of this gene family in Mytilus spp. Like many antimicrobial peptides and neurotoxins, MgCRP-I peptides are produced as pre-propeptides, usually have a net positive charge and likely derive from similar evolutionary mechanisms, that is, gene duplication and positive selection within the mature peptide region; however, synthetic MgCRP-I peptides did not display significant toxicity in cultured mammalian cells, insecticidal, antimicrobial, or antifungal activities. The functional role of MgCRP-I peptides in mussel physiology still remains puzzling

Aspetti della biologia ed analisi del ciclo riproduttivo di Gobio benacensis (Pollini, 1816) nel Nordest Italia

Il presente studio si propone di approfondire la biologia ed il ciclo riproduttivo del gobione italiano (Gobio benacensis), specie endemica a corologia cisalpina inclusa nella categoria \u201cEndangered\u201d (EN) della lista rossa IUCN, con l\u2019intento di fornire informazioni utili a redigere corretti piani gestionali volti alla conservazione della specie. Le indagini sono state condotte in un corso d\u2019acqua del Bacino del Fiume Isonzo (Torrente Reca, Nordest Italia) ed hanno riguardato 86 esemplari catturati nell\u2019aprile 2016 al fine di definirne la consistenza e la struttura della popolazione e 77 esemplari, catturati mensilmente e bimestralmente tra aprile 2016 e febbraio 2017, per la definizione del ciclo riproduttivo. Le curve di regressione del peso sulla lunghezza totale per i maschi (W=0,1253TL2,8770) e per le femmine (W=0,1555TL2,7554), confrontate mediante ANCOVA, non hanno denunciato differenze significative. I valori medi \ub1 deviazione standard dell\u2019indice gonadosomatico GSI per i maschi sono compresi tra 0,61\ub10,49 e 1,75\ub10,58, mentre per le femmine tra 1,02\ub10,88 e 14,15\ub12,80. L\u2019indice epatosomatico HSI ha denunciato range compresi tra 0,05\ub10,02 e 2,68\ub11,26 e tra 0,25\ub10,19 e 1,75\ub10,58 rispettivamente per i maschi e per le femmine. Le analisi istologiche di ovari e testicoli, analizzati per la prima volta nella specie, hanno permesso di identificare gli stadi di maturazione, che unitamente all\u2019analisi del GSI indicano che la specie depone le uova a pi\uf9 riprese durante il periodo riproduttivo compreso tra aprile e giugno. Nei maschi \ue8 stata, tuttavia, evidenziata una prolungata attivit\ue0 riproduttiva che si protrae fino al mese di agosto