Prevalência de paracoccidioidomicose-infecção determinada através de teste sorológico em doadores de sangue na região Noroeste do Paraná, Brasil

Buysson Robert du. Notes sur divers Hyménoptères. In: Bulletin de la Société entomologique de France, volume 5 (17),1900. pp. 342-343

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

The objective of this work was to evaluate biochemical parameters in Paracoccidioides brasiliensis infected mice and the effect of Canova medicine on these parameters. Mice infected with the isolate Paracoccidioides brasiliensis Pb18 and treated with Canova for 17 weeks were used. The biochemical parameters analysed were the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and amylase, and the concentration of total proteins, albumin and globulins. The results suggested that the animals that were treated with Canova had less functional alterations in their internal organs.A Paracoccidioidomicose é uma micose sistêmica endêmica em alguns países da América Latina. Essa doença afeta principalmente pulmões, gânglios e mucosas, além de causar diminuição da imunidade celular. O medicamento Canova tem sido utilizado com sucesso por pacientes imunodeprimidos, como por exemplo portadores de câncer e de AIDS. O objetivo desse trabalho foi avaliar aspectos bioquímicos em camundongos infectados com Paracoccidioides brasiliensis e os efeitos do medicamento Canova sobre esses parâmetros. Foram utilizados camundongos infectados com o isolado de Paracoccidioides brasiliensis Pb18 e tratados com o medicamento Canova durante dezessete semanas. Os parâmetros bioquímicos analisados foram níveis de atividade de aspartato amino transferase, alanina amino transferase, fosfatase alcalina e amilase, concentração de proteínas totais, albumina e globulinas. Os resultados obtidos sugerem que os animais que receberam tratamento com o medicamento Canova tiveram menores alterações funcionais nos seus órgãos internos

Highlights Regarding Host Predisposing Factors to Recurrent Vulvovaginal Candidiasis: Chronic Stress and Reduced Antioxidant Capacity.

We studied host factors that could predispose women to develop recurrent vulvovaginal candidiasis (RVVC), including glycemia, insulin resistance, chronic stress, antioxidant capacity, overall immune status, local inflammation and vaginal microbiota. The presence of yeasts in vaginal culture was screened in 277 women, with or without signs and symptoms of VVC and RVVC. The presence of an inflammatory process and microbiota were analyzed through vaginal bacterioscopy and cervical-vaginal cytology, respectively. Fasting-blood samples were collected by standard venipuncture for biochemical analyses. Flow cytometry was employed to obtain the T helper/T cytotoxic lymphocyte ratio, and insulin resistance was assessed by the HOMA index (HI). Yeasts were isolated from 71 (26%) women: 23 (32.4%) with a positive culture but without symptoms (COL), 22 (31%) in an acute episode (VVC), and 26 (36.6%) with RVVC. C. albicans was the main yeast isolated in all clinical profiles. The control group (negative culture) comprised 206 women. Diabetes mellitus and insulin resistance were more associated with the positive-culture groups (COL, VVC and RVVC) than with negative ones. The RVVC group showed lower mean levels of cortisol than the control group and lower antioxidant capacity than all other groups. The T Helper/T cytotoxic lymphocyte ratio was similar in all groups. The RVVC group showed a similar level of vaginal inflammation to the control group, and lower than in the COL and VVC groups. Only the CVV group showed a reduction in vaginal lactobacillus microbiota. Our data suggest that both chronic stress (decreased early-morning cortisol levels) and reduced antioxidant capacity can be host predisposing factors to RVVC

Cervical-vaginal cytology and bacterioscopy findings related to inflammation process and vaginal microbiota, respectively.

<p>A. Percentage of women with inflammatory processes demonstrated in microscopic analysis (400X) of vaginal cytology stained by Papanicolaou, according to clinical profiles. Samples were considered positive for inflammatory processes when smears showed leukocyte and cellular-inflammation characteristics of moderate and/or intense grade, in at least 20 different fields under optical microscopy at 400x magnification. Women with RVVC had a similar rates of vaginal inflammatory process in relation to women of control group (<i>p</i> = 0.188; OR = 1.96; 95% CI, 0.83–0.87%), while more women with VVC and COL had a vaginal inflammatory process than the control ones (*<i>p</i> = 0.0001; OR = 9.47; 95% CI, 4.03–16.44) and (*<i>p</i> = 0.0003; OR = 5.22; 95% CI, 1.97–8.69). B. Percentage of women with abundant vaginal <i>Lactobacillus</i> spp. by bacterioscopy, according to clinical profiles. Women with RVVC had no reduction in the quantities of <i>Lactobacillus</i> spp. compared to control group (<i>p</i> = 0.5223; OR = 1.429; 95% CI, 0.593–3.44).</p

Selected epidemiologic characteristics of 277 women who participated of study.

<p>Selected epidemiologic characteristics of 277 women who participated of study.</p