Feeding regimes affected the circadian rhythms of pancreatic digestive enzymes and somatic growth in flathead grey mullet (Mugil cephalus) fry

The effect of different feeding regimes on the circadian rhythms of pancreatic digestive enzyme activities was evaluated in Mugil cephalus fry weighting 0.34 ± 0.01 g. Feeding regimes (feeding ration = 3% stocked biomass) differed on the number of meals offered per day: one, two and ten meals per day (R1, R2 and R3, respectively). The number of meals per day affected somatic growth; in particular, fry from the R3 group (0.80 ± 0.01 g) grew better than their congeners from R2 (0.70 ± 0.01 g) and R1 (0.63 ± 0.01 g) groups (P < 0.05). Feeding behaviour was modulated by the feeding regime, being the maximal gut fullness values found just after meal distribution in R1 and R2 groups, whereas this trend was not observed when feed was offered continuously during light hours (R3). Fry from R1 and R2 groups showed hyperphagia as they tended to store in their gut as much as possible feed particles to be later digested due to the limited daily meals. This strategy negatively affected feed digestion due to inappropriate enzyme to substrate ratio, changes in digestive enzyme activities and chyme transit times, which ultimately impaired growth performance. Enzyme activities were modulated by the number of meals, the more frequent the meals offered, the lower enzyme activities, supporting the hypothesis that digestive function is adapted to obtain a maximum benefit of the ingested nutrients. Present results showed that feeding grey mullet fry continuously during day light hours optimized feed digestion and promoted fry growth.info:eu-repo/semantics/acceptedVersio

Editorial: Physiological Impacts of Global Warming in Aquatic Organisms

Climate change is reshaping our planet.Warming surface waters, acidification, and deoxygenation are the most critical effects of climate change in aquatic environments. Increasing mean water temperatures modify species distribution, alters their basal metabolic rates, the occurrence and intensity of marine diseases, and the timing of pivotal biological events, among others. Ocean acidification results in physiological stress and inhibits the growth and calcification of endo- and exo-skeletons, while ocean deoxygenation, and particularly hypoxic events, may alter the distribution, aerobic scope, and survival of organisms (Reid et al., 2019). As climate change is projected to continue over this century and beyond, it is expected that the above-mentioned stressors will be intensified, further altering the structure, and functioning of marine ecosystems (Benedetti et al., 2021). Understanding and predicting the effects of climate change is one of the most pressing challenges in marine science, since this knowledge has an impact on fisheries, aquaculture, conservation, and applied ecology. Under this scenario, this Research Topic was conceived for updating and increasing the knowledge of ocean water rise on the biology and physiology of aquatic species, resulting in a Research Topic of four works on crustaceans, fish, and phytoplankton.Versión del edito

Improving pikeperch larviculture by combining environmental, feeding and populational factors

Pikeperch (Sander lucioperca) has a high potential for inland aquaculture diversification in Europe. Bottlenecks (i.e. low survival, cannibalism and deformity rates) hamper further expansion of pikeperch culture, because of the weak production performances at the nursery level. To improve the production of pikeperch juveniles under recirculation system we used a pilot scale larval rearing system (700 L tanks) and multifactorial designs. Three successive larval rearing trials (duration: 35-49 days) were conducted to identify the best combination of environmental, feeding and population factors. Considering the main significant effects observed on survival, growth, swim bladder inflation rates and biomass gain, a favourable combination of twelve factor (F) modalities was proposed (F1- initial density: 100 larvae.L-1, F2- no sorting of fish jumper, F3- no sibling population, F4- eggs from large females, F5- discontinuous feeding, F6- no co-feeding, F7- light intensity: 50 lx, F8- beginning of the weaning at 16 dph (days post-hatching), F9- weaning duration: 9 days, F10- water renewal rate of 100 % per hour, F11- tank cleaning during morning and F12- tank bottom-up water current). A final validation step was realized over a last trial (seven replicates, duration: 49 days), and validated with the best productive results obtained over the global experimental period (2015-2018). These results were: a final body weight of 815.64 ± 95.34 mg, a survival rate of 16.9 ± 1.7 %, a specific growth rate of 15.1 ± 5.9 %.d-1, a final fish biomass of 9.55 ± 0.23 kg, a swim bladder inflation rate of 92.6 ± 3.2 % and a food conversion rate of 0.65 ± 0.02 (dry food). The final stocking density was 13.6 kg.m-3 of rearing volume. Authors were able to validate and provide a reliable basic protocol for pikeperch larval rearing using recirculating units.info:eu-repo/semantics/publishedVersio

Dyke or pipe? Contributions of magnetic fabrics to the reconstruction of the geometry of an eroded subvolcanic body (Cadí basin, Pyrenees)

The reconstruction of the geometry of igneous bodies in inverted basins is often a difficult task, because most of their volume can be either below or above the topographic surface. Indirect methods, such as the determination of their internal fabric can give clues to constrain geometrical reconstructions. The Sant Salvador intrusion, located in the Late Carboniferous-Permian Cadí basin (Central-Eastern Pyrenees), is a good example for testing the application of magnetic fabrics to shallow igneous bodies because of (i) its characteristics in terms of crystal composition/orientation, showing a homogeneous petrofabric controlled by plagioclase crystals 0.4 mm in size, (ii) its good, though partial, preservation, (iii) its position in the stratigraphical succession, between volcaniclastic sediments and unconformably overlying red beds, and (iv) the many unknown factors related to the intrusion, including the geometry of the body and its depth and the amount of erosion after intrusion. Furthermore, the Sant Salvador intrusion provides opportunity for checking the correspondence between the petrofabric and the magnetic fabrics when the magnetic carriers of susceptibility (with an average value of 5217·10−6 SI) are mainly ferromagnetic. In this case the main magnetic carriers are magnetite and also hematite, the latter resulting from oxidation processes at the paleosurface. The results of Anisotropy of Magnetic Susceptibility show a bimodality in the K1 disposition after bedding restitution: vertical in the deeper areas of the intrusion and subhorizontal, E-W, in the uppermost stratigraphic positions with an intermediate necking zone in which transition from vertical to horizontal flow is interpreted to occur. Bands deflecting the mineral foliation at the micro scale have also a tectonic or thermal origin but do not seem to interfere with the magnetic ellipsoid axes. Columnar jointing is interpreted to be parallel to the long axis of the intrusion (and therefore to magma flow, and perpendicular to the paleosurface. All these features indicate that the Sant Salvador intrusion can be interpreted as a subvertical pipe feeding a sill that was eroded before the sedimentation of the overlying red beds

Is a cannibal different from its conspecifics? A behavioural, morphological, muscular and retinal structure study with pikeperch juveniles under farming conditions

Cannibalism is defined as the act of killing and consuming the whole, or major part, of an individual belonging to the same species, irrespective of its stage of development. Intra-cohort cannibalism in fish larval or juvenile stages, which is a major economic problem, has been widely studied in captive fish populations. In our study, we investigated the influence of animal personality (with cross-maze and conspecific choice tests) on intra-cohort cannibalism using pikeperch Sander lucioperca as a model species. Furthermore, we investigated the morphological (geometric morphological analysis) and anatomical (histological analysis of retinal and muscle tissue sections) differences between cannibal (C) fish (TL = 34.6 ± 9.4 mm, n = 25) and conspecific fish randomly sampled from rearing tanks, herein called ‘potential non-cannibal fish’ (PNC) (TL = 31.4 ± 10.5 mm, n = 42). We did not find any behavioural differences (swimming activity, exploration, conspecific choice) between cannibal and potential non-cannibal fish that could explain asynchrony in the onset of cannibalism. Moreover, we did not observe any morphological differences between the two groups (C and PNC fish). However, we did detect anatomical differences in two retinal layers (ganglion cell layer and inner nuclear layer) that were thicker for cannibals. These two layers are involved in the collection of information by photoreceptors and allow the shapes, colours and movements of objects to be detected in the water column. The onset of cannibalism therefore appears to be linked to environmental condition-dependent individual development, with some individuals exhibiting precocious anatomical, and probably physiological, development, rather than to individual personality.info:eu-repo/semantics/acceptedVersio

Effects of soy protein base diet supplemented with lysine and methionine on digestive enzymes activity and hematological parameters in silvery-black porgy (Sparidentex hasta) juveniles

The effect of dietary partial replacement of fish meal (FM) by soybean protein (SP) alone or in combination with lysine (Lys) and methionine (Met) supplementation were tested in a 60-days feeding trial for silvery-black porgy (Sparidentex hasta) juveniles. Seven isoproteic (ca. 50% crude protein) and isoenergetic (ca. 22.4 MJ/kg) diets were formulated in which 45% (SP45), 60% (SP60) and 75% (SP75) of FM protein were replaced by SP and the control diet (FM) was prepared with FM as the major source of protein. In SP45+, SP60+ and SP75+ diets, 45 to 75% of FM was replaced by SP with supplementing blends of Lys and Met (98% of purity). The activities of the trypsin, lipase and α-amylase were higher in fish fed SP diets with crystalline amino acids supplementation than in the other groups (P < 0.05). Fish fed SP75 and SP75+ diets had the lowest red blood cell count and hematocrit level (P < 0.05). The results of the current study indicated that anti-nutritional factors in a soy-protein based diet rather than lysine and methionine deficiencies may have adverse effects on digestive enzymes activities and health condition in silvery-black porgy juveniles.info:eu-repo/semantics/publishedVersio

Lysine and methionine supplementation in high soy protein content diets for silvery-black porgy (Sparidentex hasta) juveniles

A two-month feeding trial was conducted to evaluate the effect of partial replacement of fish meal (FM) by soybean protein (SP) alone or in combination with lysine (Lys) and methionine (Met) supplementation in practical diets for silvery-black porgy juveniles (16.7±0.1 g). Seven isoproteic (ca. 50% crude protein) and isoenergetic (ca. 22.4 MJ kg-1) diets were formulated in which 45% (SP45), 60% (SP60) and 75% (SP75) of FM protein were replaced by SP and the control diet (FM) was prepared with FM as the major source of protein. In SP45+, SP60+ and SP75+ diets, 45 to 75% of FM was replaced by SP with supplementing blends of Lys and Met. Growth performance, feed utilization, and protein and lipid digestibility decreased with increasing dietary SP levels (p<0.05). Fish fed SP75 had the highest whole body lipid content, but with the lowest whole body arginine, lysine, histidine, phenylalanine and taurine concentrations (p<0.05). Supplementing Lys and Met in SP based diets did not improve growth performance, suggesting that the anti-nutritional factors in soybean protein products rather than Met and Lys deficiency might limit FM substitution with SP in silvery-black porgy juveniles.info:eu-repo/semantics/publishedVersio

Ontogeny of the digestive tract in stinging catfish, Heteropneustes fossilis (Bloch) larvae

Heteropneustes fossilis (Bloch) is an important candidate species for diversification of freshwater aquaculture in India. However, high mortality rate during larval rearing is the most serious bottleneck in commercial production of this species. A proper understanding of the ontogenic development of digestive system provides the basis to understand the nutritional physiology of larvae and develop appropriate feeding strategies. In the present study, the ontogenical development of the digestive tract in H. fossilis larvae was studied from hatching until 30 day post-hatching (dph) at 29 °C. At hatching (2.8 ± 0.2 mm standard length, SL), the digestive tract was undifferentiated and attached dorsally to the yolk sac. At 1 dph (2.9 ± 0.2 mm SL), the mouth opened and oral valves were visible. At 2 dph (3.0 ± 0.3 mm SL), goblet cells were observed in the buccoparyngaeal cavity. At this age, exogenous feeding started and the intestine was differentiated into the anterior and posterior regions, and the rudimentary liver and pancreas were also seen. Small supranuclear vacuoles were observed in the enterocytes of the posterior intestine at 2 dph. Zymogen granules were observed in acinar cells of pancreas by 3 dph, and islets of Langerhans were visible at 4 dph (3.5 ± 0.1 mm SL). At the same age, most of the yolk sac reserves were consumed, whereas they were completely exhausted by 5 dph (3.9 ± 0.5 mm SL). Between 4 and 6 dph, the liver elongated in size and started to accumulate lipids in the hepatocytes. Gastric glands were detected at 4 dph, and the pyloric sphincter was completely differentiated at 9 dph (6.1 ± 0.4 mm SL) as an epithelial fold that separated stomach from the anterior intestine. By 13 dph (8.6 ± 0.2 mm SL), profuse gastric glands were visible inside longitudinal mucosal folds of the stomach. The formation of gastric glands and their development were noticed as the last events in the development of the digestive tract in H. fossilis. This indicated the end of the larval period and the commencement of the juvenile stage. Considering these observations, it is suggested that H. fossilis larvae have a morphologically complete digestive tract by 13 dph. The findings of the study on the development of the digestive system in H. fossilis may help in synchronising the larval stage of development and feeding strategies and would be helpful in improving larval rearing techniques for catfish species.info:eu-repo/semantics/acceptedVersio

Spray-dried plasma promotes growth, modulates the activity of antioxidant defenses, and enhances the immune status of gilthead sea bream (Sparus aurata) fingerlings

Terrestrial animal byproduct meals, including nonruminant blood meal and blood products, represent the largest and largely untapped safe source of animal protein available within the international market for the aquafeed industry. Spray-dried blood and spray-dried plasma (SDP) proteins have long been recognized as high-quality feed ingredients for farmed animals. In this study, we evaluated the inclusion of SDP from porcine blood (SDPP) in growing diets for gilthead sea bream. Three isonitrogenous (CP = 51.2%) and isolipidic (fat = 12.4%) diets manufactured by cold extrusion (0.8 to 1.5 mm pellet size) were prepared by substituting high-quality fish meal with 0, 3, and 6% SDPP. The diets were tested for a period of 60 d at 22°C with 4 replicates each (400-L cylindroconical tanks, 150 fish per tank, and initial density = 0.5 kg/ m3). The SDPP inclusion in diets for gilthead sea bream fingerlings were evaluated in terms of growth performance, feed utilization, histological organization of the intestinal mucosa, activity of oxidative stress enzymes (catalase, glutathione S-transferase, glutathione peroxidase, and glutathione reductase) in the intestine, and nonspecific serum immune parameters (lysozyme and bactericidal activity). Results from this study indicated that dietary SDPP promoted fish growth in terms of BW and length; fish fed 3% SDPP were 10.5% heavier (P < 0.05) than those fed the control diet. Spray-dried plasma from porcine blood modulated the activity of the antioxidative defenses in the intestine (P < 0.05) and increased the density of goblet cells in the intestine (P < 0.05) and benefited the host by providing an effective immune barrier against gut pathogenic microbiota. The nonspecific serum immune response in fish fed diets with SDPP was greater (P < 0.05) than in fish fed the control diet. These results indicated that the inclusion of SDPP in gilthead sea bream feed could be beneficial for the fish by enhancing intestinal and serum innate immune function and the activity of antioxidative stress enzymes of the intestine and promoting growth performance.info:eu-repo/semantics/publishedVersio