Influence of Pongamia, Mahua and Neem cakes on finger millet productivity and soil fertility

A field experiment conducted at Bio-fuel park, Agricultural Research Station, Madenur, Hassan in Kharif season of 2009 to asses the performance of finger millet (Eleusine coracana L.) under different organic manure treatment consisting of four treatments viz., recommended FYM and NPK through inorganic fertilizers as control, Pongamia, Mahua and Neem cake with 5 replications laid in randomized complete block design. The results revealed that application of recommended FYM along with neem cake equivalent to 100% recommended N performedbetter in respect of finger millet productivity and maintenance of soil fertility followed by recommended FYM with 100% NPK through fertilizers. Nutrient supplementation with different oilcakes proved superior in respect of soil sustainability

Transport properties of Layer-Antiferromagnet CuCrS2: A possible thermoelectric material

The electrical, thermal conductivity and Seebeck coefficient of the quenched, annealed and slowly cooled phases of the layer compound CuCrS2 have been reported between 15K to 300K. We also confirm the antiferromagnetic transition at 40K in them by our magnetic measurements between 2K and 300K. The crystal flakes show a minimum around 100K in their in-plane resistance behavior. For the polycrystalline pellets the resistivity depends on their flaky texture and it attains at most 10 to 20 times of the room temperature value at the lowest temperature of measurement. The temperature dependence is complex and no definite activation energy of electronic conduction can be discerned. We find that the Seebeck coefficient is between 200-450 microV/K and is unusually large for the observed resistivity values of between 5-100 mOhm-cm at room temperature. The figure of merit ZT for the thermoelectric application is 2.3 for our quenched phases, which is much larger than 1 for useful materials. The thermal conductivity K is mostly due to lattice conduction and is reduced by the disorder in Cu- occupancy in our quenched phase. A dramatic reduction of electrical and thermal conductivity is found as the antiferromagnetic transition is approached from the paramagnetic region, and K subsequently rises in the ordered phase. We discuss the transport properties as being similar to a doped Kondo-insulator

U(1) Gauge Theory as Quantum Hydrodynamics

It is shown that gauge theories are most naturally studied via a polar decomposition of the field variable. Gauge transformations may be viewed as those that leave the density invariant but change the phase variable by additive amounts. The path integral approach is used to compute the partition function. When gauge fields are included, the constraint brought about by gauge invariance simply means an appropriate linear combination of the gradients of the phase variable and the gauge field is invariant. No gauge fixing is needed in this approach that is closest to the spirit of the gauge principle. We derive an exact formula for the condensate fraction and in case it is zero, an exact formula for the anomalous exponent. We also derive a formula for the vortex strength which involves computing radiation corrections.Comment: 15 pages, Plain LaTeX, final published versio

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

CRISPR/Cas9 technology enables the rapid generation of loss-of-function mutations in a targeted gene in mammalian cells. A single cell harboring those mutations can be used to establish a new cell line, thereby creating a CRISPR-induced knockout clone. These clonal cell lines serve as crucial tools for exploring protein function, analyzing the consequences of gene loss, and investigating the specificity of biological reagents. However, the successful derivation of knockout clones can be technically challenging and may be complicated by multiple factors, including incomplete target ablation and interclonal heterogeneity. Here, we describe optimized protocols and plasmids for generating clonal knockouts in mammalian cell lines. We provide strategies for guide RNA design, CRISPR delivery, and knockout validation that facilitate the derivation of true knockout clones and are amenable to multiplexed gene targeting. These protocols will be broadly useful for researchers seeking to apply CRISPR to study gene function in mammalian cells. © 2019 The Authors. © 2019 The Authors

Single-Particle Green Functions in Exactly Solvable Models of Bose and Fermi Liquids

Based on a class of exactly solvable models of interacting bose and fermi liquids, we compute the single-particle propagators of these systems exactly for all wavelengths and energies and in any number of spatial dimensions. The field operators are expressed in terms of bose fields that correspond to displacements of the condensate in the bose case and displacements of the fermi sea in the fermi case. Unlike some of the previous attempts, the present attempt reduces the answer for the spectral function in any dimension in both fermi and bose systems to quadratures. It is shown that when only the lowest order sea-displacement terms are included, the random phase approximation in its many guises is recovered in the fermi case, and Bogoliubov's theory in the bose case. The momentum distribution is evaluated using two different approaches, exact diagonalisation and the equation of motion approach. The novelty being of course, the exact computation of single-particle properties including short wavelength behaviour.Comment: Latest version to be published in Phys. Rev. B. enlarged to around 40 page

A Boost for the Emerging Field of RNA Nanotechnology: Report on the First International Conference on RNA Nanotechnology

This Nano Focus article highlights recent advances in RNA nanotechnology as presented at the First International Conference of RNA Nanotechnology and Therapeutics, which took place in Cleveland, OH, USA (October 23-25, 2010) (http;//www.eng.uc.edu/nanomedidne/RNA2010/), chaired by Peixuan Guo and co-chaired by David Rueda and Scott Tenenbaum. The conference was the first of its kind to bring together more than 30 invited speakers in the frontier of RNA nanotechnology from France, Sweden, South Korea, China, and throughout the United States to discuss RNA nanotechnology and Its applications. It provided a platform for researchers from academia, government, and the pharmaceutical industry to share existing knowledge, vision, technology, and challenges in the field and promoted collaborations among researchers interested in advancing this emerging scientific discipline. The meeting covered a range of topics, including biophysical and single-molecule approaches for characterization of RNA nanostructures; structure studies on RNA nanoparticles by chemical or biochemical approaches, computation, prediction, and modeling of RNA nanoparticle structures; methods for the assembly of RNA nanoparticles; chemistry for RNA synthesis, conjugation, and labeling; and application of RNA nanoparticles in therapeutics. A special invited talk on the well-established principles of DNA nanotechnology was arranged to provide models for RNA nanotechnology. An Administrator from National Institutes of Health (NIH) National Cancer Institute (NCI) Alliance for Nanotechnology in Cancer discussed the current nanocancer research directions and future funding opportunities at NCl. As indicated by the feedback received from the invited speakers and the meeting participants, this meeting was extremely successful, exciting, and informative, covering many groundbreaking findings, pioneering ideas, and novel discoveries