159 research outputs found
Computational analysis of Variable Thrust Engine (VTE) performance
- Publication venue
- Publication date
- Field of study
The Variable Thrust Engine (VTE) of the Orbital Maneuvering Vehicle (OMV) uses a hypergolic propellant combination of Monomethyl Hydrazine (MMH) and Nitrogen Tetroxide (NTO) as fuel and oxidizer, respectively. The performance of the VTE depends on a number of complex interacting phenomena such as atomization, spray dynamics, vaporization, turbulent mixing, convective/radiative heat transfer, and hypergolic combustion. This study involved the development of a comprehensive numerical methodology to facilitate detailed analysis of the VTE. An existing Computational Fluid Dynamics (CFD) code was extensively modified to include the following models: a two-liquid, two-phase Eulerian-Lagrangian spray model; a chemical equilibrium model; and a discrete ordinate radiation heat transfer model. The modified code was used to conduct a series of simulations to assess the effects of various physical phenomena and boundary conditions on the VTE performance. The details of the models and the results of the simulations are presented
CFD Analysis of Spray Combustion and Radiation in OMV Thrust Chamber
- Publication venue
- Publication date
- Field of study
The Variable Thrust Engine (VTE), developed by TRW, for the Orbit Maneuvering Vehicle (OMV) uses a hypergolic propellant combination of Monomethyl Hydrazine (MMH) and Nitrogen Tetroxide (NTO) as fuel and oxidizer, respectively. The propellants are pressure fed into the combustion chamber through a single pintle injection element. The performance of this engine is dependent on the pintle geometry and a number of complex physical phenomena and their mutual interactions. The most important among these are (1) atomization of the liquid jets into fine droplets; (2) the motion of these droplets in the gas field; (3) vaporization of the droplets (4) turbulent mixing of the fuel and oxidizer; and (5) hypergolic reaction between MMH and NTO. Each of the above phenomena by itself poses a considerable challenge to the technical community. In a reactive flow field of the kind occurring inside the VTE, the mutual interactions between these physical processes tend to further complicate the analysis. The objective of this work is to develop a comprehensive mathematical modeling methodology to analyze the flow field within the VTE. Using this model, the effect of flow parameters on various physical processes such as atomization, spray dynamics, combustion, and radiation is studied. This information can then be used to optimize design parameters and thus improve the performance of the engine. The REFLEQS CFD Code is used for solving the fluid dynamic equations. The spray dynamics is modeled using the Eulerian-Lagrangian approach. The discrete ordinate method with 12 ordinate directions is used to predict the radiative heat transfer in the OMV combustion chamber, nozzle, and the heat shield. The hypergolic reaction between MMH and NTO is predicted using an equilibrium chemistry model with 13 species. The results indicate that mixing and combustion is very sensitive to the droplet size. Smaller droplets evaporate faster than bigger droplets, leading to a well mixed zone in the combustion chamber. The radiative heat flux at combustion chamber and nozzle walls are an order of negligible less than the conductive heat flux. Simulations performed with the heat shield show that a negligible amount of fluid is entrained into the heat shield region. However, the heat shield is shown to be effective in protecting the OMV structure surrounding the engine from the radiated heat
Combustion chamber analysis code
- Publication venue
- Publication date
- Field of study
A three-dimensional, time dependent, Favre averaged, finite volume Navier-Stokes code has been developed to model compressible and incompressible flows (with and without chemical reactions) in liquid rocket engines. The code has a non-staggered formulation with generalized body-fitted-coordinates (BFC) capability. Higher order differencing methodologies such as MUSCL and Osher-Chakravarthy schemes are available. Turbulent flows can be modeled using any of the five turbulent models present in the code. A two-phase, two-liquid, Lagrangian spray model has been incorporated into the code. Chemical equilibrium and finite rate reaction models are available to model chemically reacting flows. The discrete ordinate method is used to model effects of thermal radiation. The code has been validated extensively against benchmark experimental data and has been applied to model flows in several propulsion system components of the SSME and the STME
Amyloid precursor-like protein 2 (APLP2) affects the actin cytoskeleton and increases pancreatic cancer growth and metastasis.
- Author
- Batra Surinder K.
- Caplan Steve
- Das Srustidhar
- Giridharan Sai Srinivas Panapakkam
- Haridas Dhanya
- Hollingsworth Michael A.
- Kaur Sukhwinder
- MacDonald Richard G.
- McKenna Nicole R.
- Meza Jane L
- Naslavsky Naava
- Pan Zenggang
- Pandey Poomy
- Peters Haley L.
- Rachagani Satyanarayana
- Radhakrishnan Prakash
- Seshacharyulu Parthasarathy
- Sheinin Yuri
- Smith Brittney L.
- Solheim Joyce C.
- Publication venue
- DigitalCommons@UNMC
- Publication date
- 11/12/2014
- Field of study
Amyloid precursor-like protein 2 (APLP2) is aberrantly expressed in pancreatic cancer. Here we showed that APLP2 is increased in pancreatic cancer metastases, particularly in metastatic lesions found in the diaphragm and intestine. Examination of matched human primary tumor-liver metastasis pairs showed that 38.1% of the patients had positive APLP2 expression in both the primary tumor and the corresponding liver metastasis. Stable knock-down of APLP2 expression (with inducible shRNA) in pancreatic cancer cells reduced the ability of these cells to migrate and invade. Loss of APLP2 decreased cortical actin and increased intracellular actin filaments in pancreatic cancer cells. Down-regulation of APLP2 decreased the weight and metastasis of orthotopically transplanted pancreatic tumors in nude mice
Novel substituted methylenedioxy lignan suppresses proliferation of cancer cells by inhibiting telomerase and activation of c-myc and caspases leading to apoptosis
- Author
- A Balakrishnan
- A Strasser
- CB Thompson
- D Broccoli
- D Rachid
- F Marks
- G Evan
- GI Evan
- GM Cragg
- J Liu
- JW Shay
- K Narayanan
- K Perumal
- Kang Boem Kown
- M Barnett
- MA White
- MS Soengas
- N P Karthikeyan
- NB Mulchandani
- NO Karpinich
- NW Kim
- NW Kim
- P Giridharan
- P Juin
- P Schotte
- R A Vishwakarma
- R Velmurugan
- S Haldar
- S T Somasundaram
- TG Graeber
- YE Keqiang
- Publication venue
- Nature Publishing Group
- Publication date
- Field of study
Conventional solvent fractionation and bioactivity based target assays were used to identify a new anti-cancer molecule from Phyllanthus urinaria, a herbal medicinal plant used in South India. At each step of the purification process the different fractions that were isolated were tested for specific anti-proliferative activity by assays measuring the inhibition of [3H]thymidine incorporation, and trypan blue drug exclusion. The ethyl acetate fraction that contained the bioactivity was further purified and resolved by HPLC on a preparative column. The purity of each of the fractions and their bioactivity were checked. Fraction 3 demonstrated a single spot on TLC and showed maximum anti-proliferative activity. This fraction was further purified and the structure was defined as 7âČ-hydroxy-3âČ,4âČ,5,9,9âČ-pentamethoxy-3,4-methylene dioxy lignan using NMR and mass spectrometry analysis. The pure compound and the crude ethyl acetate fraction which showed anti-proliferative activities were examined for ability to target specific markers of apoptosis like bcl2, c-myc and caspases and for effects on telomerase. Four specific cancer cell lines HEp2, EL-1 monocytes, HeLa and MCP7 were used in this study. The results indicate that 7âČ-hydroxy-3âČ,4âČ,5,9,9âČ-pentamethoxy-3,4-methylene dioxy lignan was capable of inhibiting telomerase activity and also could inhibit bcl2 and activate caspase 3 and caspase 8 whose significance in the induction of apoptosis is well known. We believe that this compound could serve as a valuable chemotherapeutic drug after further evaluations
Reactions to symptoms of mental disorder and help seeking in Sabah, Malaysia.
- Author
- Publication venue
- 'SAGE Publications'
- Publication date
- 01/01/2017
- Field of study
Abstract Background: A better understanding is needed about how people make decisions about help seeking. Materials: Focus group and individual interviews with patients, carers, healthcare staff, religious authorities, traditional healers and community members. Discussion: Four stages of help seeking were identified: (1) noticing symptoms and initial labelling, (2) collective decision-making, (3) spiritual diagnoses and treatment and (4) psychiatric diagnosis and treatment. Conclusion: Spiritual diagnoses have the advantage of being less stigmatising, giving meaning to symptoms, and were seen to offer hope of cure rather than just symptom control. Patients and carers need help to integrate different explanatory models into a meaningful whole.N/
Biological properties of Endophytic Fungi
- Author
- Abraham S
- Aguilar AC
- Arivudainambi USE
- Arnold AE
- Barraquio WL
- Baskar K
- Bensaci OA
- Boddey RM
- Bungihan ME
- Chen ZM
- Devaraju R
- dos Santos RMG
- FirĂĄkovĂĄ S
- Giridharan P
- Goodman RN
- Guo B
- Hamayun M
- Huang WY
- Jayanthi G
- Johnson MC
- Kathirvelu Baskar
- Khan AL
- Khan AR
- Kumaresan V
- Larran S
- Li JY
- Li X
- Liang H
- Loiret FG
- Lu H
- Lv Y
- Ma Y
- Malinowski DP
- Malinowski DP
- Meng X
- Naif Abdullah Al-Dhabi
- Nath A
- Oses R
- Page M
- Pandi M
- Patil MP
- Pimentel MR
- Posada F
- Ramar Govindaraj
- Rodriguez RJ
- Ruma K
- Sandhiya GS
- Santiago C
- Schardl CL
- Selim KA
- Senthilkumar N
- Shi YW
- Soleimani M
- Soleimani M
- Strobel G
- Strobel G
- Sturz AV
- Suja M
- Tayung K
- Tomita F
- Veeramuthu Duraipandiyan
- Vega FE
- Venkatesan Sudha
- Wang FW
- Wani MC
- Webber J
- Zhang HW
- Zhao J
- Publication venue
- 'FapUNIFESP (SciELO)'
- Publication date
- 01/01/2016
- Field of study
Hepatoprotective efficacy of Hypnea muciformis ethanolic extract on CCl4 induced toxicity in rats
- Author
- Anggadiredja J
- Blunden G
- Borzelleca JF
- Bousquet BF
- Boyd CE
- Boyd EM
- Cavin C
- Chhaya Gadgoli
- Chinnaiah Amutha
- Chun-Kwan W
- Fossati P
- Giridharan Bupesh
- Girish S achliya
- Gornall A
- Harris RN
- Indegaard M
- Kind PN
- King J
- Klaassen CD
- Konig GM
- Lahaye M
- Luly P
- Malloy HT
- Matsukawa R
- Naidoo K
- Natarajan Kavithalakshmi
- Natesan Manoharan
- Ohkawa Î
- Ooi VEC
- Opoku AR
- Periyasamy Subramanian
- Raju Krishnamoorthy
- Ramalingam Senthil Raja
- Recknagel R
- Reitman S
- Sakthivel Vasanth
- Siva Kumar K
- Slater TF
- Smiddsrod R
- Snedecor GW
- Uday B
- Upadhyay RK
- Yan X
- Publication venue
- 'FapUNIFESP (SciELO)'
- Publication date
- Field of study
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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- A. -G. Wu
- A. C. Ma
- A. K. Au
- Abdel-Aziz A. K.
- Abdelfatah S.
- Abdellatif M.
- Abdoli A.
- Abel S.
- Abeliovich H.
- Abildgaard M. H.
- Abudu Y. P.
- Acevedo-Arozena A.
- Adamopoulos I. E.
- Adeli K.
- Adolph T. E.
- Adornetto A.
- Aflaki E.
- Agam G.
- Agarwal A.
- Aggarwal B. B.
- Agnello M.
- Agostinis P.
- Agrewala J. N.
- Agrotis A.
- Aguilar P. V.
- Ahmad S. T.
- Ahmed Z. M.
- Ahumada-Castro U.
- Aits S.
- Aizawa S.
- Akkoc Y.
- Akoumianaki T.
- Akpinar H. A.
- Al-Abd A. M.
- Al-Akra L.
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- Alaoui-Jamali M. A.
- Alberti S.
- Alcocer-Gomez E.
- Alessandri C.
- Ali M.
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- Aliwaini S.
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- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
- Abdoli A
- Abel S
- Abeliovich H
- Abildgaard MH
- Abudu YP
- Acevedo-Arozena A
- Adamopoulos IE
- Adeli K
- Adolph TE
- Adornetto A
- Aflaki E
- Agam G
- Agarwal A
- Aggarwal BB
- Agnello M
- Agostinis P
- Agrewala JN
- Agrotis A
- Aguilar PV
- Ahmad ST
- Ahmed ZM
- Ahumada-Castro U
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- Aizawa S
- Akkoc Y
- Akoumianaki T
- Akpinar HA
- Al-Abd AM
- Al-Akra L
- Al-Gharaibeh A
- Alaoui-Jamali MA
- Alberti S
- Alcocer-GĂłmez E
- Alessandri C
- Ali M
- Alim Al-Bari MA
- Aliwaini S
- Alizadeh J
- Almacellas E
- Almasan A
- Alonso A
- Alonso GD
- Altan-Bonnet N
- Altieri DC
- Alves da Costa C
- Alves S
- Alzaharna MM
- Amadio M
- Amantini C
- Amaral C
- Ambrosio S
- Amer AO
- Ammanathan V
- An Z
- Andersen SU
- Andrabi SA
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- Angelini S
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- Anozie UC
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- Zhu B
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- Zhuang H
- Zhuang X
- Zientara-Rytter K
- Zimmermann CM
- Ziviani E
- Zoladek T
- Zong W-X
- Zorov DB
- Zorzano A
- Zou W
- Zou Z
- Zou Z
- Zuryn S
- Zwerschke W
- Ălvarez ĂMC
- Ăvalos Y
- Ănal G
- ĂstĂŒn S
- ÄoliÄ M
- ÄokiÄ J
- Ćœerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
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