3 research outputs found
Quality of Lupinus albus L. (White Lupin) Seed: Extent of Genotypic and Environmental Effects
White
lupin seed can be used for traditional and functional foods
or as animal feed. This study aimed to support lupin breeders and
production stakeholders by assessing the extent of genotypic, environmental,
and genotype × environment (GE) interaction effects on seed contents
of oil, tocopherols (TOC), and quinolizidine alkaloids (QA), grain
yield, and seed weight of eight elite genotypes grown in two climatically
contrasting Italian locations for two cropping years. On average,
plants in the subcontinental climate site exhibited higher grain yield
and seed size, about 8% lower oil content, and almost 85% higher QA
content than those in the Mediterranean climate site. The range of
genotype means was 2.97–5.14 t/ha for yield, 92–110
mg/g for oil, and 0.121–0.133 mg/g for TOC. TOC amount was
largely unpredictable and featured large GE interactions that hinder
its genetic improvement. Oil and alkaloid contents and seed size are
more predictable and offer potential for selection
Exploration of Potentially Bioactive Peptides Generated from the Enzymatic Hydrolysis of Hempseed Proteins
The
seed of industrial hemp is an underexploited protein source. In view
of a possible use in functional foods, a hempseed protein concentrate
was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these
enzymes. A detailed peptidomic analysis using data-dependent acquisition
showed that the numbers of peptides identified ranged from 90 belonging
to 33 parent proteins in the peptic hydrolysate to 9 belonging to
6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates
resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme
A reductase activity when tested on the catalytic domain of the enzyme.
Using the open access tools PeptideRanker and BIOPEP, a list of potentially
bioactive peptides was generated: the alleged activities included
the antioxidant property, the glucose uptake stimulating activity,
the inhibition of dipeptidyl peptidase-IV and angiotensin-converting
enzyme I
New ACE-Inhibitory Peptides from Hemp Seed (<i>Cannabis sativa</i> L.) Proteins
A hemp
seed protein isolate, prepared from defatted hemp seed meals
by alkaline solubilization/acid precipitation, was subjected to extensive
chemical hydrolysis under acid conditions (6 M HCl). The resulting
hydrolysate was fractionated by semipreparative RP-HPLC, and the purified
fractions were tested as inhibitors of angiotensin converting enzyme
(ACE). Mono- and bidimensional NMR experiments and LC-MS analyses
led to the identification of four potentially bioactive peptides,
i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis,
and tested for ACE-inhibitory activity. The IC<sub>50</sub> values
were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and
RVR 526 ± 33 μM, confirming that hemp seed may be a valuable
source of hypotensive peptides