Ciencia en tu salón

Ciencia en tu salón es un espacio de UMH Sapiens que contiene muchas actividades ypasatiempos interesantes relacionados con el mundo científico en castellano, valenciano einglés. Por ejemplo, retos como el que os proponemos a continuación: hacer que unpequeño huerto urbano crezca en cas

CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803

Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux–resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, CopM, and a two-component system CopRS, which controls the expression of these two operons. Here, we report that CopM is a periplasmic protein able to bind Cu(I) with high affinity (KD ∼3 × 10−16). Mutants lacking copM showed a sensitive copper phenotype similar to mutants affected in copB, but lower than mutants of the two-component system CopRS, suggesting that CopBAC and CopM constitute two independent resistance mechanisms. Moreover, constitutive expression of copM is able to partially suppress the copper sensitivity of the copR mutant strain, pointing out that CopM per se is able to confer copper resistance. Furthermore, constitutive expression of copM was able to reduce total cellular copper content of the copR mutant to the levels determined in the wild-type (WT) strain. Finally, CopM was localized not only in the periplasm but also in the extracellular space, suggesting that CopM can also prevent copper accumulation probably by direct copper binding outside the cell.España, Ministerio de Economía y Competitividad BFU2010–15708España, Ministerio de Economía y Competitividad BFU2013–41712-

Ciencia en tu salón

Ciencia en tu salón es un espacio de UMH Sapiens que contiene muchas actividades ypasatiempos interesantes relacionados con el mundo científico en castellano, valenciano einglés. Por ejemplo, retos como el que os proponemos a continuación: hacer que unpequeño huerto urbano crezca en cas

Divus Thomas cum patribus ex prophetis locutus ... sive Dissertationes Theologicae scholastico-dogmaticae et mystico-doctrinales ... Divi Thomae ... [Texto impreso]

Sign.: [Calderon]-2[calderon]4, 3[calderon]2, A-Zzz4, Aaaa-Llll4Port. a dos tintas y con esc. xil. arzobispalTexto a dos col.Las il. son grab. calc.: "Joseph Bergara delin. J. Giner fet. Val. Añño 1750"en ¶2 retrato del Papa Benedicto XIV con S. Pedro y S. Pablo, y otro grab. calc. en p. 1 de Santo Tomás de Aquino: "R. fc. Valae. 1746

Redox control of copper homeostasis in cyanobacteria

Copper is essential for all living organisms but is toxic when present in excess. Therefore organisms have developed homeostatic mechanism to tightly regulate its cellular concentration. In a recent study we have shown that CopRS two-component system is essential for copper resistance in the cyanobacterium Synechocystis sp PCC 6803. This two-component regulates expression of a heavy-metal RND type copper efflux system (encoded by copBAC) as well as its own expression (in the copMRS operon) in response to an excess of copper in the media. We have also observed that both operons are induced under condition that reduces the photosynthetic electron flow and this induction depends of the presence of the copper-protein, plastocyanin. These findings, together with CopS localization to the thylakoid membrane and its periplasmic domain being able to bind copper directly, suggest that CopS could be involved in copper detection in both the periplasm and the thylakoid lume

Memorias historicas del Antiguo Santuario del Santo Sepulcro de Valencia

Sign.: *8, **4, A-I8, K7Grab. xil. en p. 10 y 14La h. de lám.: "Joaqn. Giner

EMG, Rate of Perceived Exertion, Pain, Tolerability and Possible Adverse Effects of a Knee Extensor Exercise with Progressive Elastic Resistance in Patients with Severe Haemophilia

In people with haemophilia (PWH), elastic band training is considered an optimal option, even though the literature is scarce. The aim was to evaluate normalized electromyographic amplitude (nEMG), rate of perceived exertion (RPE), pain, tolerability, and possible adverse effects during the knee extension exercise using multiple elastic resistance intensities in PWH. During a single session, 14 severe PWH undergoing prophylactic treatment performed knee extensions without resistance and with different intensity levels of elastic resistance. nEMG was measured for the knee extensors and participants rated their RPE, tolerability and pain intensity after each condition. Patients had to report the possible adverse effects after the session. In most of the cases, an nEMG increase is only evidenced after increasing the resistance by two to three levels. Significant associations were found between RPE and the nEMG (ρ = 0.61), as well as between the elastic resistance level and nEMG (ρ = 0.69) and RPE (ρ = 0.71). All conditions were generally tolerated, without increased pain, and no adverse effects were reported. A wide variety of elastic resistance intensities during the knee extension are safe, tolerated, and do not increase knee pain in the majority of severe PWH undergoing prophylactic treatment

Safety, Fear and Neuromuscular Responses after a Resisted Knee Extension Performed to Failure in Patients with Severe Haemophilia

Background: low–moderate intensity strength training to failure increases strength and muscle hypertrophy in healthy people. However, no study assessed the safety and neuromuscular response of training to failure in people with severe haemophilia (PWH). The purpose of the study was to analyse neuromuscular responses, fear of movement, and possible adverse effects in PWH, after knee extensions to failure. Methods: twelve severe PWH in prophylactic treatment performed knee extensions until failure at an intensity of five on the Borg CR10 scale. Normalised values of amplitude (nRMS) and neuromuscular fatigue were determined using surface electromyography for the rectus femoris, vastus medialis, and vastus lateralis. After the exercise, participants were asked about their perceived change in fear of movement, and to report any possible adverse effects. Results: Patients reported no adverse effects or increased fear. The nRMS was maximal for all the muscles before failure, the median frequency decreased, and wavelet index increased during the repetitions. The vastus lateralis demonstrated a higher maximum nRMS threshold and earlier fatigue, albeit with a lower and more progressive overall fatigue. Conclusions: severe PWH with adequate prophylactic treatment can perform knee extensions to task failure using a moderate intensity, without increasing fear of movement, or adverse effects

Dental extractions in patients on antiplatelet therapy. A study conducted by the Oral Health Department of the Navarre Health Service (Spain)

Objectives: Antiplatelet drugs are used to treat and prevent a wide range of cardiovascular pathologies and/or cerebrovascular accidents. Although the use of anticoagulants in dental extractions is highly protocolized, a clear control method has not yet been established for antiplatelet drugs. This study is directed at evaluating the clinical consequences of extractions in patients on antiplatelet therapy. Study design: The Oral Health Department of the Navarre Health Service-Osasunbidea conducted a trial on 155 patients who underwent dental extractions and were receiving antiplatelet therapy. The patients were not requested to interrupt the medication and local measures were taken to control potential haemorrhage. Results: No major haemorrhages were reported. One patient had a moderate haemorrhage that required emergency care. In the remaining patients the bleeding was controlled with local measures. With regard to subsequent bleeding, no differences were observed between the various antiplatelet drugs used. The only statistically significant relationship found was between bleeding and the number of teeth extracted. Conclusions: It can be concluded that no more than 3 teeth should be removed at any one time, and for multiple extractions, the teeth should be adjacent to each other