RODENT HEMOGLOBIN STRUCTURE: A COMPARISON OF SEVERAL SPECIES OF MICE *

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72885/1/j.1749-6632.1974.tb21897.x.pd

Gz, a guanine nucleotide-binding protein with unique biochemical properties

Cloning of a complementary DNA (cDNA) for Gz alpha, a newly appreciated member of the family of guanine nucleotide-binding regulatory proteins (G proteins), has allowed preparation of specific antisera to identify the protein in tissues and to assay it during purification from bovine brain. Additionally, expression of the cDNA in Escherichia coli has resulted in the production and purification of the recombinant protein. Purification of Gz from bovine brain is tedious, and only small quantities of protein have been obtained. The protein copurifies with the beta gamma subunit complex common to other G proteins; another 26- kDa GTP-binding protein is also present in these preparations. The purified protein could not serve as a substrate for NAD-dependent ADP- ribosylation catalyzed by either pertussis toxin or cholera toxin. Purification of recombinant Gz alpha (rGz alpha) from E. coli is simple, and quantities of homogeneous protein sufficient for biochemical analysis are obtained. Purified rGz alpha has several properties that distinguish it from other G protein alpha subunit polypeptides. These include a very slow rate of guanine nucleotide exchange (k = 0.02 min^-1), which is reduced greater than 20-fold in the presence of mM concentrations of Mg2+. In addition, the rate of the intrinsic GTPase activity of Gz alpha is extremely slow. The hydrolysis rate (kcat) for rGz alpha at 30 degrees C is 0.05 min^-1, or 200-fold slower than that determined for other G protein alpha subunits. rGz alpha can interact with bovine brain beta gamma but does not serve as a substrate for ADP-ribosylation catalyzed by either pertussis toxin or cholera toxin. These studies suggest that Gz may play a role in signal transduction pathways that are mechanistically distinct from those controlled by the other members of the G protein family

Factorization Contributions and the Breaking of the ΔI=1/2\Delta I=1/2 Rule in Weak ΛNρ\Lambda N\rho and ΣNρ\Sigma N\rho Couplings

We compute the modified factorization contributions to the $\Lambda\rightarrow N\rho$ and $\Sigma\rightarrow N\rho$ couplings and demonstrate that these contributions naturally include $\Delta I=3/2$ terms which are comparable ($\simeq 0.4$ to $-0.8$ times) in magnitude to the corresponding $\Delta I=1/2$ terms. As a consequence, we conclude that models which treat vector meson exchange contributions to the weak conversion process $\Lambda N\rightarrow NN$ assuming such weak couplings to satisfy the $\Delta I=1/2$ rule are unlikely to be reliable.Comment: 13 pages, uses REVTEX Entire manuscript available as a ps file at http://www.physics.adelaide.edu.au/theory/home.html . Also available via anonymous ftp at ftp://adelphi.adelaide.edu.au/pub/theory/ADP-95-5.T172.ps To appear in Physical Review

Observations on mold development and on deterioration in stored yellow dent shelled corn

Examinations during 3 consecutive years were made in Iowa for mold development in yellow dent shelled corn stored in steel bins of 1,000-2,740 bushel capacities. The first was made during August and September 1941, the second in March 1942 and the third in April 1943. Different bins were examined in each of the three inspections. Bins reportedly containing some corn of 14 percent or more moisture were examined and probed in the first examination, those with corn of 13.5 percent or more moisture in the second examination; no selection of bins for moisture content was made in the third examination. All of the bins examined had been filled during the fall previous to sampling

SU(3) Decomposition of Two-Body B Decay Amplitudes

We present the complete flavor SU(3) decomposition of decay amplitudes for decays of the triplet (B^+_u, B^0_d, B^0_s) of B mesons nonleptonically into two pseudoscalar mesons. This analysis holds for arbitrarily broken SU(3) and can be used to generate amplitude relations when physical arguments permit one to neglect or relate any of the reduced amplitudes.Comment: 31 pages, revtex, no figure

Kinematic dynamo action in a sphere. I. Effects of differential rotation and meridional circulation on solutions with axial dipole symmetry

A sphere containing electrically conducting fluid can generate a magnetic field by dynamo action, provided the flow is sufficiently complicated and vigorous. The dynamo mechanism is thought to sustain magnetic fields in planets and stars. The kinematic dynamo problem tests steady flows for magnetic instability, but rather few dynamos have been found so far because of severe numerical difficulties. Dynamo action might, therefore, be quite unusual, at least for large-scale steady flows. We address this question by testing a two-parameter class of flows for dynamo generation of magnetic fields containing an axial dipole. The class of flows includes two completely different types of known dynamos, one dominated by differential rotation (D) and one with none. We find that 36% of the flows in seven distinct zones in parameter space act as dynamos, while the remaining 64% either fail to generate this type of magnetic field or generate fields that are too small in scale to be resolved by our numerical method. The two previously known dynamo types lie in the same zone, and it is therefore possible to change the flow continuously from one to the other without losing dynamo action. Differential rotation is found to promote large-scale axisymmetric toroidal magnetic fields, while meridional circulation (M) promotes large-scale axisymmetric poloidal fields concentrated at high latitudes near the axis. Magnetic fields resembling that of the Earth are generated by D > 0, corresponding to westward flow at the surface, and M of either sign but not zero. Very few oscillatory solutions are found

Determination of CKM phases through rigid polygons of flavor SU(3) amplitudes

Some new methods for the extraction of CKM phases $\alpha$ and $\gamma$ using flavor SU(3) symmetry have been suggested through the construction of rigid polygons in the complex plane with sides equal to the decay amplitudes of B mesons into two mesons belonging to the light (charmless) pseudoscalar octet. These rigid polygons incorporate all the possible amplitude triangles and, being overdetermined, also serve as consistency checks and in estimating the rates of some decay modes. The same techniques also lead to numerous useful amplitude triangles when octet-singlet mixing has been taken into account and nearly physical $\eta,\eta'$ are used.Comment: A few detailed explanations added, some rearrangement of sections and a few minor changes in notation. 19 pages, 1 PostScript figure, uses psfig.st

Rare Kaon Decays in the 1/Nc1/N_c-Expansion

We study the unknown coupling constants that appear at order $p^4$ in the Chiral Perturbation Theory analysis of $K \to \pi \gamma^* \to \pi l^+ l^-$, $K^{+-} \to \pi^{+-} \gamma \gamma$ and $K \to \pi \pi \gamma$ decays. To that end, we compute the chiral realization of the $\Delta S \, = \, 1$ Hamiltonian in the framework of the $1/N_c$-expansion of the low-energy action. The phenomenological implications are also discussed.Comment: 18 pages, LaTeX, CPT-92/P.279