The Space of Reception: Framing Autonomy and Collaboration

In this paper we analyse the ideas implicit in the style of exhibition favoured by contemporary galleries and museums, and argue that unless the audience is empowered to ascribe meaning and significance to artwork through critical dialogue, the power not only of the audience is undermined but also of art. We argue that galleries and museums preside over an experience economy devoid of art, unless (i) indeterminacy is understood, (ii) the critical rather than coercive nature of art is facilitated, and (iii) the conditions for inter-subjectivity are met

Molecular Biological Studies On The Rep Gene Of Escherichia Coli

My work has involved the sequencing of the Escherichia coli rep gene. This gene codes for the Rep helicase, a ssDNA-dependent ATPase required by some phages ({dollar}\Phi{dollar}X174, fd, P2) for their replication.;The section of E. coli chromosome known to contain the rep gene, which I have sequenced, has only one transcribed open reading frame of the correct size to encode the Rep protein. The predicted N-terminal amino acids and the predicted total amino acid composition are in agreement with those determined from purified Rep protein.;The proposed amino acid sequence of Rep contains in its N terminus a peptide common to all ATP-binding enzymes: G/A-X{dollar}\sb4{dollar}-GKT-X{dollar}\sb6{dollar}-I. It is also possible to use the primary amino acid sequence data to predict a secondary structure for the Rep protein. The amino acid sequence of the Rep protein was compared with others already present in the protein sequence data bank; DNA helicase II the uvrD gene product, was identified as the only protein with significant homology with Rep.;In order to correlate structural features with functional protein domains two approaches were used. The first approach involved creating minor deletions in the gene and assaying for some of Rep\u27s activities.;The second approach has been to investigate the dasC mutation (das stands for dnaA suppressor). This mutation had been mapped to the vicinity of the rep gene. The dasC phenotype (growth at 42{dollar}\sp\circ{dollar}C of an E. coli strain carrying the dnaA46 temperature sensitive allele) appears to be due to a lesion in the DNA downstream from the rep gene dasC which represses the ability of Rep to complement the dnaA46 mutant. The rep alleles isolated from dasC and wild type strains confer the temperature-resistant phenotype

Gestural Abstraction in Australian Art 1947 – 1963: Repositioning the Work of Albert Tucker

Gestural abstraction in the work of Australian painters was little understood and often ignored or misconstrued in the local Australian context during the tendency‟s international high point from 1947-1963. Distinguished by the mark of the artist evident in the finished work, gestural abstraction included a number of international variations such as European tachisme or l’art informel, l’art brut, matière or matter painting and American Abstract Expressionism. This thesis will examine the reasons for the lack of significance attributed to gestural abstraction by Australian art critics and art historians by taking a unique analytical approach which is developed in the first half of the thesis. This approach draws on interdisciplinary sources to establish a framework for repositioning examples of Australian painting which would be better appreciated as examples of gestural abstraction. The work of Australian artist Albert Tucker produced during the relevant period will be the main focus in the artist chapters. This thesis will demonstrate that certain art works can benefit from a reclassification which brings out qualities which enhance the perceived significance of the work. Such reconfiguration of elements can lead to better informed reception. Art historical approaches have not as a rule focused on aesthetic theory, concentrating rather on art historical precedent. This can involve overlooking the relevant social and historical aspects of the work‟s making and reception in favour of dominant styles favoured at the time or location of reception. Once the critical precedent is set for an artist‟s work of a particular period, it can be difficult to revise; such is the role of precedent in art criticism of certain periods, particularly within Australia at the time of this study. What is deemed the relevant art historical precedent for any given work in part determines how the work is received. However, this is rarely acknowledged. Instead, art historical classifications eventually are treated as objective properties of the work. Aesthetic theory, in contrast, reveals the contingency of such classifications. In addition to philosophical aesthetics, this thesis will draw on sociological field theory to account for the dynamic process through which aesthetic categorisations become active. This reveals the relevant contextual elements necessary to confirm the appropriate category, enabling optimal placement of the work to best appreciate its historical significance. A robust analytical methodology is developed on the basis of which art historical accounts can be evaluated. This involves examining the Australian art historiography and curatorial practices which led to the perception of these works in categories other than those in which they were produced and initially exhibited by the artist.Thesis (Ph.D.) -- University of Adelaide, Art History, 201

Azabicyclo[3.2.0]heptan-7-ones (carbapenams) from pyrrole

The azabicyclo[3.2.0]heptan-7-ones 4, 10, 16 and 24 have been prepared from pyrrole. The same general approach has been used for all these derivatives; namely, substitution of pyrrole at the 2- and 5-carbon atoms, catalytic hydrogenation to produce pyrrolidine-2-acetic acid derivatives, and cyclisation using tris(1,3-dihydro-2-oxobenzoxazol-3-yl)phosphine oxide 6. The catalytic hydrogenation of 2,5-disubstituted pyrroles gives only the corresponding cis-2,5-disubstituted pyrrolidines. The hydrogenation proceeds more easily when the nitrogen atom bears a tert-butoxycarbonyl substituent. The N-tert-butoxycarbonylpyrroles 8 and 21 bearing an á-substituent in the acetate side chain were hydrogenated with a high degree of facial stereoselectivity. This allowed the 6-phthalimidoazabicyclo- [3.2.0]heptan-7-one 24 to be isolated as a single diastereoisomer. The X-ray crystal structure of a precursor, the triester, 22a, has been obtained

Entamoeba bangladeshi nov. sp., Bangladesh.

: TO THE EDITOR: Diarrheal diseases have a major effect on global health, particularly the health of malnourished children (1). The enteric parasites Entamoeba histolytica and E. moshkovskii are potential causes of diarrheal disease in children (2). For the past 20 years, we have been studying Entamoeba infections in children from the urban slum of Mirpur in Dhaka, Bangladesh (3)

Regulation of Virulence of Entamoeba histolytica by the URE3-BP Transcription Factor

It is not understood why only some infections with Entamoeba histolytica result in disease. The calcium-regulated transcription factor upstream regulatory element 3-binding protein (URE3-BP) was initially identified by virtue of its role in regulating the expression of two amebic virulence genes, the Gal/GalNac lectin and ferredoxin. Here we tested whether this transcription factor has a broader role in regulating virulence. A comparison of in vivo to in vitro parasite gene expression demonstrated that 39% of in vivo regulated transcripts contained the URE3 motif recognized by URE3-BP, compared to 23% of all promoters (P < 0.0001). Amebae induced to express a dominant positive mutant form of URE3-BP had an increase in an elongated morphology (30% ± 6% versus 14% ± 5%; P = 0.001), a 2-fold competitive advantage at invading the intestinal epithelium (P = 0.017), and a 3-fold increase in liver abscess size (0.1 ± 0.1 g versus 0.036 ± 0.1 g; P = 0.03). These results support a role for URE3-BP in virulence regulation

Rapid assessment of tetanus vaccine-induced immunity in Bangladesh and the Gambia.

We have developed recombinant fragment C based rapid point of care dipstick devices to assess tetanus immunization status using plasma or whole blood. The devices demonstrated specificity of 0.90 and sensitivity of 0.90 (whole blood)/0.94 (plasma) at field sites in Bangladesh and The Gambia when compared to a commercial ELISA with the immune cut-off titer set as ≥0.1IU/mL

Targets of the Entamoeba histolytica Transcription Factor URE3-BP

The Entamoeba histolytica transcription factor Upstream Regulatory Element 3-Binding Protein (URE3-BP) is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (Upstream Regulatory Element 3 (URE3)) in the promoter regions of hgl5 and fdx1. In this work, precise definition of the consensus URE3 element was performed by electrophoretic mobility shift assays (EMSA) using base-substituted oligonucleotides, and the consensus motif validated using episomal reporter constructs. Transcriptome profiling of a strain induced to produce a dominant-positive URE3-BP was then used to identify additional genes regulated by URE3-BP. Fifty modulated transcripts were identified, and of these the EMSA defined motif T[atg]T[tc][cg]T[at][tgc][tg] was found in over half of the promoters (54% p<0.0001). Fifteen of the URE3-BP regulated genes were potential membrane proteins, suggesting that one function of URE3-BP is to remodel the surface of E. histolytica in response to a calcium signal. Induction of URE3-BP leads to an increase in tranwell migration, suggesting a possible role in the regulation of cellular motility