Heliconius doris RSEM mapped count data

File is count data (expression level) output of Trinity contigs from RSEM for Heliconius doris. Column headers contain species name, sex (M or F), specimen ID (see Table S1 for details), and tissue type ("ant" = antennae, "leg" = legs, and "mou" = mouthparts)

Heliconius doris Trinity assembly

This is a fasta format file containing all mRNA contigs for Heliconius doris. The assembly was performed in Trinity version "20130216" and is a raw output that includes all assembled contigs (see Table 1 for contig counts). Assembly was carried out as described in the Methods section of the publication

Heliconius sara Trinity assembly

This is a fasta format file containing all mRNA contigs for Heliconius sara. The assembly was performed in Trinity version "20130216" and is a raw output that includes all assembled contigs (see Table 1 for contig counts). Assembly was carried out as described in the Methods section of the publication

Eueides isabella Trinity assembly

This is a fasta format file containing all mRNA contigs for Eueides isabella. The assembly was performed in Trinity version "20130216" and is a raw output that includes all assembled contigs (see Table 1 for contig counts). Assembly was carried out as described in the Methods section of the publication

Eueides isabella RSEM mapped count data

File is count data (expression level) output of Trinity contigs from RSEM for Eueides isabella. Column headers contain species name, sex (M or F), specimen ID (see Table S1 for details), and tissue type ("ant" = antennae, "leg" = legs, and "mou" = mouthparts)

Heliconius erato RSEM mapped count data

File is count data (expression level) output of Trinity contigs from RSEM for Heliconius erato. Column headers contain species name, sex (M or F), specimen ID (see Table S1 for details), and tissue type ("ant" = antennae, "leg" = legs, and "mou" = mouthparts)

Heliconius melpomene Trinity assembly

This is a fasta format file containing all mRNA contigs for Heliconius melpomene. The assembly was performed in Trinity version "20130216" and is a raw output that includes all assembled contigs (see Table 1 for contig counts). Assembly was carried out as described in the Methods section of the publication

Annotation file of open reading frames from 5 butterfly species

This is an annotation file for open reading frames (ORFs) from five species of heliconiine butterflies. The file includes ORFs from all five species, top BLAST hits, and the ortholog group the ORF belongs to. Column headers are as follows: "Row#" (row number), "Seq_ID" (sequence ID, including: 3 letter species code [Eis = Eueides isabella, Hdo = Heliconius doris, Her = Heliconius erato, Hmel = Heliconius melpomene, Hsa = Heliconius sara], Trinity 'gene' contig ID [e.g., compXXXX_cX], and ORF coordinates within contig), "H-ID" (hit ID), "H-Eval" (hit e-value), "H-Desc" (hit description), "H-Species" (hit species), "H-Type" (hit type), "H-Tag" (hit database), "H-Len" (hit length), and "OM" (orthocluster membership). NOTE: The program TCW produces two BLAST hits: 1. Best e-value, 2. best e-value with best evidence. The ones presented here are number 2

Heliconius sara RSEM mapped count data

File is count data (expression level) output of Trinity contigs from RSEM for Heliconius sara. Column headers contain species name, sex (M or F), specimen ID (see Table S1 for details), and tissue type ("ant" = antennae, "leg" = legs, and "mou" = mouthparts)

IMa2 input files

Input files for sequence analysis in IMa2. Files in the archive include 3 pairwise comparisons of populations from Smith et al data and 3 from Machado et al (2007), labelled by population comparison (see manuscript) and data source. The last file is for comparison of all populations for the Smith et al data. All files contain locus ID, DNA sequence (aligned using MAFFT - see manuscript for details) and locus specific mutation rates. Line 1= general info, line 2 = number of populations, line 3 = tree topology, line 4 = number of loci, thereafter = sequence data