Nutritional Characteristics of \u3ci\u3eBrachiaria ruziziensis\u3c/i\u3e (Germain & Evrard) Subjected to Different Doses of Gamma Rays

Appropriate methods of mutation induction are of high importance in pastures for increased genetic variability and improved forage performance. The objective of this research was to improve agronomic and nutritive performance of mutant lines through induced mutagenesis to seeds of a local landrace Brachiaria ruziziensis. The seeds were irradiated with 0, 10, 20, 30, and 40Gy doses of gamma radiation from Cobalt 60 (60Co). Treatments were; KE 0Gy, KE 10Gy, KE 20Gy, KE 30Gy, and KE 40Gy resulting into M1 seeds. The seeds were planted in the greenhouse in germination pots then transplanted to the field. Seeds of M6 plants (M7 seeds) were used to establish field experiment in a completely randomized block design, with three replications. Parameters measured included; tillering, leaf-stem ratio, dry matter, mineralogy, digestibility, and crude protein. Data collected was analyzed using Genstat version 18 software. Growth parameters that were affected by radiation included tillering and days to 50% flowering. Radiation increased neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude protein (CP). NDF had high positive correlation with ADF, CP had high positive correlation with leaf length. Results from agronomic performance, nutrient profiles indicated that the mutant lines differed from their parents. The mutant lines could be selected, multiplied and used in feeding trials to determine reproductive performance of dairy cattle fed on the mutant lines. Future studies should focus on total mixed ration formulation using the mutant lines, designed feeding trials, fractionation of amino and fatty acids from the lines for incorporation in poor forages and national performance trials of mutant lines with an aim of forage variety release. Application of nuclear technology to other grasses would lead to increased biomass and improved nutrition for increased animal productivity leading to food and nutrition security

Out of Site out of Mind: Quantifying the Long-term Off-site economic Impacts of Land Degradation in Kenya

This study investigated the private and social returns to Sustainable Land Management (SLM) practices with an objective of finding practices that reduce the on-farm and off-farm negative effects of land degradation. The results show that SLM practices have robust profits for farmers raising dairy cows. Farmers without dairy cows realize profits that are sensitive to input and output prices. Adoption of SLM also provides global environmental services whose value is about 10% of the net present value. The results suggest the need to promote SLM practices with multiple uses and consider ways to compensate farmers who offer significant environmental services.Environmental Economics and Policy,

CIRCULATORY CYTOKINES AND HEMATOLOGICAL PROFILES: POSSIBLE BIOMARKERS OF HIV/AIDS DISEASE PROGRESSION

Introduction: This study sought to identify circulatory cytokines and hematological profiles measureable in blood plasma in newly diagnosed HIV patients as possible biomarkers that could predict the progression of HIV and AIDS disease in the course of acute HIV infection.Methodology: A prospective cross sectional study design was used to recruit the participants at the Nakuru Provincial General hospital in Kenya.  The study group included those who were HIV positive before and after commencing therapy and those who were HIV negative. The study group composed of male and female of different ages ranging from 7-72 years.  Hematology auto analyzer system was used to analyze hematological parameters and indices.  Types and concentrations of cytokines were determined using multiplex cytokine immunoassay by flow cytometry using Becton and Dickinsonfluorescence activated cell sorter (BD FACS) count.  Descriptive statistics were applied and a p- value < 0.05 was considered statistically significant.Results: This study found a significant difference in mean Interleukin 12p70 (p<0.001), Tumor Necrosis Factor (p<0.05), Interleukin 10 (p<0.05), Interleukin 6 (p<0.005) and interleukin 1β (p<0.05) between HIV negative patients, treatment naïve HIV patients and HIV patients on highly active antiretroviral therapy (HAART). Among the treatment naïve HIV patients, significant associations were observed between IL-12p70 and HGB (p<0.05); between TNF and MPV (p<0.001); between IL-10 and PDW (p<0.005); between IL-6 and Gran# (p-0.05); between IL-1β and PDW (p<0.005).Conclusion: The early period of infection with HIV is characterized by high circulatory cytokines levels and could be useful biomarkers and indicators of early immune activation of HIV infection. The results from this study also showed that acute HIV infection induces several hematological changes, involving all the blood parameters and indices, some of which may act as indicators of HIV/AIDS disease progression

The Performance of Rapid Diagnostic Test for Malaria Parasite Diagnosis Compared to Microscopic Test in Meru South Sub-County, Tharaka-Nithi County, Kenya

Despite intensive worldwide attempt to control malaria, it remains one of the most fatal and widespread protozoan infection of mankind. About 2.4 billon people inhabit malaria prone regions which is about forty percentage of the world population in over 90 countries of Sub-Saharan Africa are affected. Prompt accurate, diagnosis and treatment is important to avert suffering of patients and malaria infection is a serious global challenge in the affected countries. The rapid diagnosis test of malaria is a recent diagnostic technique whose performance has not been evaluated in Meru South Sub-County. The main purpose of this research study was to perform immunosurveillance and evaluate performance rapid diagnosis test for malaria parasites in Meru South Sub-County, Tharaka-Nithi County. The study design was hospital based cross-sectional study in the laboratory at Chuka Level Five Hospital. Three hundred and eighty four blood specimens were used from febrile patients with clinical manifestation of malaria infection. The blood specimens were used for thin, thick smear and rapid diagnosis test. The results were analyzed by t-test to compare the mean of the two methods. A P –value of 0.953 was obtained which is greater than 0.05, therefore we accept the null hypothesis that there is no difference in performance between the Rapid Diagnostic Test (RDT) and microscopic test. The results indicated that RDT had similar performance with microscopy for both positive and negative cases of malaria infection. In conclusion RDT is appropriate for malaria diagnosis since the incidence rate of malaria was found to be high and the predominant Plasmodium falciparum was high in the study area.  The researcher recommends the use of RDTs in mass screening for malaria infection, adopt or intensify protective measures during dry seasons and monitoring antimalaria drug resistance or tolerance in all counties in Kenya

Cytokine expression in malaria-infected non-human primate placentas

Malaria parasites are known to mediate the induction of inflammatory immune responses at the maternal-foetal interface during placental malaria (PM) leading to adverse consequences like pre-term deliveries and abortions. Immunological events that take place within the malaria-infected placental micro-environment leading to retarded foetal growth and disruption of pregnancies are among the critical parameters that are still in need of further elucidation. The establishment of more animal models for studying placental malaria can provide novel ways of circumventing problems experienced during placental malaria research in humans such as inaccurate estimation of gestational ages. Using the newly established olive baboon (Papio anubis)-Plasmodium knowlesi (P. knowlesi) H strain model of placental malaria, experiments were carried out to determine placental cytokine profiles underlying the immunopathogenesis of placental malaria. Four pregnant olive baboons were infected with blood stage P. knowlesi H strain parasites on the one fiftieth day of gestation while four other uninfected pregnant olive baboons were maintained as uninfected controls. After nine days of infection, placentas were extracted from all the eight baboons through cesarean surgery and used for the processing of placental plasma and sera samples for cytokine sandwich enzyme linked immunosorbent assays (ELISA). Results indicated that the occurrence of placental malaria was associated with elevated concentrations of tumour necrosis factor alpha (TNF-{\alpha}) and interleukin 12 (IL-12). Increased levels of IL-4, IL-6 and IL-10 and interferon gamma (IFN-{\gamma}) levels were detected in uninfected placentas. These findings match previous reports regarding immunity during PM thereby demonstrating the reliability of the olive baboon-P. knowlesi model for use in further studies.Comment: Open Veterinary Journal 1st June 2012. Seven pages, Three Figures. arXiv admin note: text overlap with arXiv:1201.323

Effects Of HIV and Intestinal Parasites Co-Infection On Hematological Parameters Among Pregnant Women Attending Selected Health Facilities In Nyeri County, Kenya

 Introduction: Prevalence of HIV among women in Nyeri County increased from 2.5% in 2007 to 5.5 % in 2009 and 6.3% in 2013. The study aimed to determine effect of co-infection of HIV and intestinal parasites on hemogram among pregnant women attending health facilities in the County. Methodology: A comparative cross-sectional study was conducted among 130 participants. Interview schedule was used to collect data. Stool and blood samples were processed using standard procedures. Data was analyzed using SPSS. Results among 130 respondents 34% had intestinal protozoans infection. Results: Among 65 HIV positive respondents, 25% had Entamoeba Coli infection and 2% Iodamoeba butschlii. Among 65 HIV negative respondents, 38% had Entamoeba Coli, and 6% Iodamoeba butschlii infection. One HIV negative respondent had Hymenolepis nana infection. Co-infection of HIV and intestinal parasites had significant effect on WBC (p < 0.05), RBC (p < 0.05), Haemoglobin (p < 0.05) and haematocrit (p < 0.05). Conclusion: (i) Prevalence of co-infection of intestinal protozoan parasites and HIV was high(ii) Co-infection of HIV and intestinal protozoan parasites decreased WBC, RBC, haemoglobin and haematocrit. Recommendation: Routine screening for intestinal parasites during antenatal healthcare and more research to verify pathogenicity of Entamoeba Coli. Key words: HIV, Intestinal parasites, co-infection, pregnant women, hemogram

Genetic diversity of Kenyan Prosopis populations based on random amplified polymorphic DNA markers

Several Prosopis species and provenances were introduced in Kenya, either as a single event or repeatedly. To date, naturally established Prosopis populations are described as pure species depending on site, despite the aforementioned introduction of several species within some sites. To determine whether naturally established stands consist of a single or mixture of species, six populations from Bamburi, Bura, Isiolo, Marigat, Taveta and Turkwel were compared for relatedness with reference to Prosopis chilensis, Prosopis juliflora and Prosopis pallida using random amplified polymorphic DNA markers. Cluster analysis based on Nei’s genetic distance clustered Kenyan populations as follows: Marigat, Bura and Isiolo with P. juliflora, Bamburi with P. pallida and Taveta with P. chilensis, whereas the Turkwel population is likely to be a hybrid between P. chileneis and P. juliflora. Four populations had private markers, revealing germplasm uniqueness. Expected heterozygosity tended to be larger for Kenyan populations (ranging from 0.091 to 0.191) than in the three reference (ranging from 0.065 to 0.144). For the six Kenyan populations and two P. juliflora provenances from the Middle East, molecular variation was larger within populations than between population. Higher molecular variance among populations is attributed to their geographical separation and the low variation within populations is due to gene flow between individuals within a population. Overall, this study shows that (1) the Kenyan Prosopis populations are genetically isolated, (2) multiple introductions enhanced genetic diversity within sites and (3) P. juliflora and its hybrid are the most aggressive invaders.Key words: Prosopis chilensis, Prosopis juliflora, Prosopis pallida, multiple introductions, genetic diversity

Leishmaniasis in northern and western Africa: a review

Leishmaniasis, one of the highly neglected diseases is currently a significant health problem in northern Africa with a rising concern in western Africa because of co-infection with the Human Immunodeficiency Virus (HIV). In this review, we present a summarized analysis of the epidemiology, infective species, parasites reservoirs, diagnosis, treatment and control measures of leishmaniasis in northern and western Africa region. In northern Africa, the disease is prevalent in Morocco, Algeria, Tunisia, Egypt and Libya. Comparatively, there are low prevalence rates of the disease in West African countries including Cameroon, Ghana, Burkina Faso, Niger, Mali, Nigeria and Senegal. In North Africa, visceral leishmaniasis (VL) is caused by L. infantum and transmitted by Phlebotomus perniciosus and P. longicuspis. On the other hand, cutaneous leishmaniasis (CL) is mainly caused by L. major and transmitted by P. papatasi, P. duboscqi and P. pedifer with L. infantum and L. tropica causing lower incidences of the disease. Notably, Algeria is one of the countries that constitute 90% of CL cases worldwide. In Western Africa; CL is caused by L. major while VL is caused by L. donovani. In these regions, zoonotic and anthroponotic cutaneous and visceral leishmaniasis is a health problem that should be addressed urgently.Key words: Leishmaniasis; cutaneous leishmaniasis, visceral leishmaniasi

Protection against Cutaneous Leishmaniasis in Outbred Vervet Monkeys, Using a Recombinant Histone H1 Antigen

Infection with Leishmania major parasites results in the development of cutaneous ulcerative lesions on the skin. We investigated the protective potential of a single, recombinant histone H1 antigen against cutaneous leishmaniasis in an outbred population of vervet monkeys, using Montanide adjuvant. Protection was assessed by challenging the animals with a mixture of vector sand fly salivary-gland lysate and a low dose of in vitro-derived parasites, thus more closely mimicking natural infection induced by L. major. The course of infection in immunized monkeys was compared with that of animals that had healed from a primary infection and were immune. The monkeys immunized with recombinant histone H1 showed a reduced development of lesion size, compared with controls. Our study therefore illustrates the potential use of histone H1 as a vaccine candidate against cutaneous leishmaniasis in human

Evaluation of the Therapeutic Potential of Warburgia ugandensis, Prunus africana, and Piliostigma thonningii against Leishmania donovani in vitro and in Balb/c Mice

Leishmaniasis is a zoonotic disease caused by protozoan parasites of the genus Leishmania. Conventional chemotherapy remains to be the most preferred measure against leishmaniasis despite being associated with high toxicity and relapse rates. They are also expensive and require hospitalization. Plant-based compounds provide a better treatment alternative because they are effective, cheap, and less associated with toxicity and resistance. This study examined the therapeutic potential of Warburgia ugandensis, Prunus africana, and Piliostigma thonningii against Leishmania donovani infection in BALB/c mice. Anti-promastigote and toxicity studies were evaluated by incubating the test compound with promastigotes and Vero cells, respectively. Serum was obtained from the mice for total immunoglobulin gamma (IgG) quantification. For in vivo studies, the mice were infected with virulent Leishmania donovani then treated with methanolic extracts of Warburgia ugandensis, Prunus africana, and Piliostigma thonningii and control drug, pentostam (sodium stibogluconate). Treatment with the plant extracts and standard drug resulted to significant reduction in parasite burden. Outcomes in the mice treated with plant extracts were comparable to those treated with pentostam (P≥0.05). In the promastigote assay, all the test compounds killed more than half of the promastigotes at the highest concentration (500 µg/mL). Warburgia ugandensis, P. thonningii, and P. africana reduced the number of promastigotes from 2.0 × 106 to 7.7 × 103 , 72.0 × 103 , and 5.0 × 103 , respectively. Pentostam had the lowest IC50 (210 µg/mL), followed by Warburgia ugandensis (IC50 of 270 µg/mL). Piliostigma thonningii and P. africana were less toxic with IC50 of 720 µg/mL and 500 µg/mL, respectively. There was low production of IgG antibodies following treatment with the plant extracts and high levels in the untreated control