26 research outputs found
A remotely-operated facility for evaluation of post-combustion CO2 capture technologies on industrial sites
ACTTROM (Advanced Capture Testing in a Transportable Remotely-Operated Minilab) is a transportable test facility for bench-scale evaluation of postcombustion CO2 capture technologies using real industrial flue gases. It is designed to be remote-operable, requiring visits only once per month for maintenance and sample collection. ACTTROM is the first facility of its kind, owned and operated by academia for collaborative research in an industrial environment, and this has resulted in a number of unique developments to facilitate remote operation at an industrial host site. Specifically, it has been necessary to design the unit to automatically correct or mitigate the effects of fault conditions, and to be remotely-monitored via a user interface at 24 hour intervals
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Farnesoid X receptor and liver X receptor ligands initiate formation of coated platelets
The liver X receptors (LXRs) and farnesoid X receptor (FXR) have been identified in human platelets. Ligands of these receptors have been shown to have nongenomic inhibitory effects on platelet activation by platelet agonists. This, however, seems contradictory with the platelet hyper-reactivity that is associated with several pathological conditions that are associated with increased circulating levels of molecules that are LXR and FXR ligands, such as hyperlipidemia, type 2 diabetes mellitus, and obesity. We, therefore, investigated whether ligands for the LXR and FXR receptors were capable of priming platelets to the activated state without stimulation by platelet agonists. Treatment of platelets with ligands for LXR and FXR converted platelets to the procoagulant state, with increases in phosphatidylserine exposure, platelet swelling, reduced membrane integrity, depolarization of the mitochondrial membrane, and microparticle release observed. Additionally, platelets also displayed features associated with coated platelets such as P-selectin exposure, fibrinogen binding, fibrin generation that is supported by increased serine protease activity, and inhibition of integrin Ī±IIbĪ²3. LXR and FXR ligand-induced formation of coated platelets was found to be dependent on both reactive oxygen species and intracellular calcium mobilization, and for FXR ligands, this process was found to be dependent on cyclophilin D. We conclude that treatment with LXR and FXR ligands initiates coated platelet formation, which is thought to support coagulation but results in desensitization to platelet stimuli through inhibition of Ī±IIbĪ²3 consistent with their ability to inhibit platelet function and stable thrombus formation in vivo
Platelet factor XIII-A regulates platelet function and promotes clot retraction and stability.
Factor XIII (FXIII) is an important proenzyme in the hemostatic system. The plasma-derived enzyme activated FXIII cross-links fibrin fibers within thrombi to increase their mechanical strength and cross-links fibrin to fibrinolytic inhibitors, specifically Ī±2-antiplasmin, to increase resistance to fibrinolysis. We have previously shown that cellular FXIII (factor XIII-A [FXIII-A]), which is abundant in the platelet cytoplasm, is externalized onto the activated membrane and cross-links extracellular substrates. The contribution of cellular FXIII-A to platelet activation and platelet function has not been extensively studied. This study aims to identify the role of platelet FXIII-A in platelet function. We used normal healthy platelets with a cell permeable FXIII inhibitor and platelets from FXIII-deficient patients as a FXIII-free platelet model in a range of platelet function and clotting tests. Our data demonstrate that platelet FXIII-A enhances fibrinogen binding to the platelet surface upon agonist stimulation and improves the binding of platelets to fibrinogen and aggregation under flow in a whole-blood thrombus formation assay. In the absence of FXIII-A, platelets show reduced sensitivity to agonist stimulation, including decreased P-selectin exposure and fibrinogen binding. We show that FXIII-A is involved in platelet spreading where a lack of FXIII-A reduces the ability of platelets to fully spread on fibrinogen and collagen. Our data demonstrate that platelet FXIII-A is important for clot retraction where clots formed in its absence retracted to a lesser extent. Overall, this study shows that platelet FXIII-A functions during thrombus formation by aiding platelet activation and thrombus retraction in addition to its antifibrinolytic roles
John Grote, Cambridge University and the development of Victorian ideas, 1830 - 1870
In 2 vols.Available from British Library Document Supply Centre-DSC:DX188055 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo