Diferencias inter-individuales y variabilidad intersesión de IgA salival como potencial biomarcador del dolor en sujetos sanos bajo condiciones de ambiente controlado

Hasta el momento, el dolor solo puede medirse con escalas subjetivas puesto que es lo único que está validado, pero es evidente que se necesitan medidas objetivas de dolor. Los biomarcadores en saliva analizados mediante la recogida de muestras por secreción pasiva es un método sencillo, libre de estrés y rápido, encontrándose entre ellos la IgAs que constituye el 15% de las inmunoglobulinas séricas y encontrándose científicamente demostrada su relación con el dolor. La mejor forma de estudiar el efecto de las condiciones ambientales en humanos es la de someterlos a condiciones controladas, aislándolos del ambiente externo a través de la cámara de ambiente controlado. El objetivo de este estudio es analizar la variabilidad interindividual y la variabilidad intersesión de los valores de IgAs establecidos de dolor en la saliva de sujetos sanos sometidos a unas condiciones de ambiente controlado; 22ºC y 50% de humedad. Inicialmente se realizó una búsqueda bibliográfica e la base de datos científica de PubMed. Posteriormente, se ha realizado un estudio clínico, prospectivo y observacional en el que se recogen dos muestras de saliva de individuos sanos entre 30-40 años, ambas separadas por un tiempo mínimo de 24h y tras haber permanecido durante 30 min en una cámara de ambiente controlado (Tª 22ºC y H 50%). Se reclutaron 8 sujetos a los que se recogió muestras salivales. No se encontraron diferencias estadísticamente significativas entre el sexo para las concentraciones de IgAs (p> 0,05), CCI: 0,41 y cuyo análisis de Bland- Altman muestra que la diferencia entre las concentraciones es independiente de la media. A pesar de los resultados obtenidos en este trabajo, donde las concentraciones de IgAs muestran una fiabilidad aceptable, se continuará con el estudio aumentando la muestra de participantes, así como los biomarcadores a analizar que serán incluidos con la IgAs, la Tsal, el cortisol, la AEA y el sTNFαRII, con el fin de realizar una comparación exacta coincidiendo lo más posible con el estudio de Sobas EM, (2016).Departamento de EnfermeríaMáster en Enfermería Oftalmológic

Mishel’s Model of uncertainty describing categories and subcategories in fibromyalgia patients, a scoping review

Producción CientíficaThe aim of this review was to demonstrate the presence of categories and subcategories of Mishel’s model in the experiences of patients with fibromyalgia by reviewing qualitative studies. Uncertainty is defined as the inability to determine the meaning of disease-related events. A scoping review of qualitative studies was carried out. Twenty articles were included, with sample sizes ranging from 3 to 58 patients. Articles from different countries and continents were included. Three categories of the model and eight subcategories could be shown to be present in the experiences of fibromyalgia patients through the scoping review. The first category, concerning antecedents of uncertainty in patients with fibromyalgia, is constituted by the difficulty in coping with symptoms, uncertainty about the diagnosis and uncertainty about the complexity of the treatment. The second concerns the cognitive process of anxiety, stress, emotional disorder and social stigma. The third category refers to coping with the disease, through the management of social and family support and the relationship with health care professionals

Muscular Strength and Quality of Life in Older Adults: The Role of ACTN3 R577X Polymorphism

As longevity is increasing, the 65-year-old and older population is projected to increase in the next decades, as are the consequences of age-related muscle deterioration on the quality of life. The purpose of this study was to examine the associations of the ACTN3R577X polymorphism with quality of life and muscular strength in an older Spanish population. In total, 281 older adults participated in this study. Anthropometric measurements, chronic diseases, prescribed medications, quality of life, hand grip strength, and physical activity and nutritional status data were collected. ACTN3 R577X genotyping was determined using Taqman probes. Multivariate regression analysis revealed in adjusted model that, in men, the ACTN3 R577X genotype was significantly associated with hand grip strength (HGS), regression coefficient (β) = 1.23, p = 0.008, dimension 1 of the five-dimension questionnaire EuroQoL (EQ-5D, mobility), (β) = −1.44, p = 0.006, and clinical group risk (CGR) category (β) = −1.38, p = 0.006. In women, a marginal association between the ACTN3 R577X genotype and the CGR category was observed, with a regression coefficient of (β) = −0.97, (p = 0.024). Our findings suggest that the ACTN3 R577X genotype may influence the decline in muscle strength and quality of life in older Spanish adult males

Haplotypes in the GC, CYP2R1 and CYP24A1 Genes and Biomarkers of Bone Mineral Metabolism in Older Adults

Candidate gene studies have analyzed the effect of specific vitamin D pathway genes on vitamin D availability; however, it is not clear whether genetic variants also affect overall bone metabolism. This study evaluated the association between genetic polymorphisms in GC, CYP2R1 and CYP24A1 and serum levels of total 25(OH)D, iPTH and other mineral metabolism biomarkers (albumin, total calcium and phosphorus) in a sample of 273 older Spanish adults. We observed a significant difference between CYP2R1 rs10741657 codominant model and total 25(OH)D levels after adjusting them by gender (p = 0.024). In addition, the two SNPs in the GC gene (rs4588 and rs2282679) were identified significantly associated with iPTH and creatinine serum levels. In the case of phosphorus, we observed an association with GC SNPs in dominant model. We found a relationship between haplotype 2 and 25(OH)D levels, haplotype 4 and iPTH serum levels and haplotype 7 and phosphorus levels. In conclusion, genetic variants in CYP2R1 and GC could be predictive of 25(OH)D and iPTH serum levels, respectively, in older Caucasian adults. The current study confirmed the role of iPTH as one of the most sensitive biomarkers of vitamin D activity in vivo