183 research outputs found
Memory shapes microbial populations
Correct decision making is fundamental for all living organisms to thrive under environmental changes. The patterns of environmental variation and the quality of available information define the most favourable strategy among multiple options, from randomly adopting a phenotypic state to sensing and reacting to environmental cues. Cellular memory—the ability to track and condition the time to switch to a different phenotypic state—can help withstand environmental fluctuations. How does memory manifest itself in unicellular organisms? We describe the population-wide consequences of phenotypic memory in microbes through a combination of deterministic modelling and stochastic simulations. Moving beyond binary switching models, our work highlights the need to consider a broader range of switching behaviours when describing microbial adaptive strategies. We show that memory in individual cells generates patterns at the population level coherent with overshoots and non-exponential lag times distributions experimentally observed in phenotypically heterogeneous populations. We emphasise the implications of our work in understanding antibiotic tolerance and, in general, bacterial survival under fluctuating environments
Introspection dynamics: a simple model of counterfactual learning in asymmetric games
Social behavior in human and animal populations can be studied as an evolutionary process.Individuals often make decisions between different strategies, and those strategies that yield afitness advantage tend to spread. Traditionally, much work in evolutionary game theory considerssymmetric games: individuals are assumed to have access to the same set of strategies, and theyexperience the same payoff consequences. As a result, they can learn more profitable strategies byimitation. However, interactions are oftentimes asymmetric. In that case, imitation may beinfeasible (because individuals differ in the strategies they are able to use), or it may be undesirable(because individuals differ in their incentives to use a strategy). Here, we consider an alternativelearning process which applies to arbitrary asymmetric games,introspection dynamics. Accordingto this dynamics, individuals regularly compare their present strategy to a randomly chosenalternative strategy. If the alternative strategy yields a payoff advantage, it is more likely adopted. Inthis work, we formalize introspection dynamics for pairwise games. We derive simple and explicitformulas for the abundance of each strategy over time and apply these results to severalwell-known social dilemmas. In particular, for the volunteer’s timing dilemma, we show that theplayer with the lowest cooperation cost learns to cooperate without delay
I Servizi Ecosistemici del suolo per la pianificazione della cittĂ e del territorio
a partire dalla considerazione che la maggior parte, se non tutti, i servizi resi dal suolo hanno un’utilità diretta ed indiretta per l’uomo, e quindi una rilevanza anche in termini economici, appare quindi cruciale, nell’ambito delle politiche di gestione e di pianificazione del territorio, valutare le ricadute economiche di diverse scelte di pianificazione territoriale ed urbanistica, attraverso la stima dei costi e benefici associabili a diversi scenari di uso del suolo, e/o a politiche di tutela e indirizzi propri degli strumenti di pianificazione territoriale ed urbanistica. Partendo da questo presupposto, il progetto LIFE SAM4CP propone di contribuire ad uno degli obiettivi posti dall’UE all’interno del programma Life + 2013 nell’ambito della Politica e Governance ambientali relativo al suolo
The Ecosystem Services for a sustainable multi-level planning. The Project Life SAM4CP. 8th Conference of Ecosystem Services Partnership, 9-13 November 2015, South Africa.
The poster highlights the ways by which Ecosystem Services (ES) mapping tools and techniques can be used effectively in decision-making problems when programming and planning the city and the territory.
The supported thesis is that the analysis of ES finds the benefits of each planning option and thus help in deciding the optimum trough a multilevel governance model
GNG5 Controls the Number of Apical and Basal Progenitors and Alters Neuronal Migration During Cortical Development
Cortical development is a very complex process in which any temporal or spatial alterations can give rise to a wide range of cortical malformations. Among those malformations, periventricular heterotopia (PH) is characterized by clusters of neurons that do not migrate to the correct place. Cerebral organoids derived from patients with mutations in DCHS1 and FAT4, which have been associated with PH, exhibit higher levels of GNG5 expression in a patient-specific cluster of neurons. Here we investigate the role of GNG5 during the development of the cerebral cortex in mice and human cerebral organoids. GNG5, highly expressed in progenitors and downregulated in neurons, is critical for controlling the number of apical and basal progenitors and neuronal migration. Moreover, forced expression of GNG5 recapitulates some of the alterations observed upon downregulation of Dchs1 and Fat4 in mice and human cerebral organoids derived from DCHS1 and FAT4 patients, suggesting a critical role of GNG5 in cortical development
Sex or cannibalism: Polyphenism and kin recognition control social action strategies in nematodes
Resource polyphenisms, where single genotypes produce alternative feeding strategies in response to changing environments, are thought to be facilitators of evolutionary novelty. However, understanding the interplay between environment, morphology, and behavior and its significance is complex. We explore a radiation of Pristionchus nematodes with discrete polyphenic mouth forms and associated microbivorous versus cannibalistic traits. Notably, comparing 29 Pristionchus species reveals that reproductive mode strongly correlates with mouth-form plasticity. Male-female species exhibit the microbivorous morph and avoid parent-offspring conflict as indicated by genetic hybrids. In contrast, hermaphroditic species display cannibalistic morphs encouraging competition. Testing predation between 36 co-occurring strains of the hermaphrodite P. pacificus showed that killing inversely correlates with genomic relatedness. These empirical data together with theory reveal that polyphenism (plasticity), kin recognition, and relatedness are three major factors that shape cannibalistic behaviors. Thus, developmental plasticity influences cooperative versus competitive social action strategies in diverse animals
Non-cell-autonomous regulation of interneuron specification mediated by extracellular vesicles
Disruption in neurogenesis and neuronal migration can influence the assembly of cortical circuits, affecting the excitatory-inhibitory balance and resulting in neurodevelopmental and neuropsychiatric disorders. Using ventral cerebral organoids and dorsoventral cerebral assembloids with mutations in the extracellular matrix gene LGALS3BP, we show that extracellular vesicles released into the extracellular environment regulate the molecular differentiation of neurons, resulting in alterations in migratory dynamics. To investigate how extracellular vesicles affect neuronal specification and migration dynamics, we collected extracellular vesicles from ventral cerebral organoids carrying a mutation in LGALS3BP, previously identified in individuals with cortical malformations and neuropsychiatric disorders. These results revealed differences in protein composition and changes in dorsoventral patterning. Proteins associated with cell fate decision, neuronal migration, and extracellular matrix composition were altered in mutant extracellular vesicles. Moreover, we show that treatment with extracellular vesicles changes the transcriptomic profile in neural progenitor cells. Our results indicate that neuronal molecular differentiation can be influenced by extracellular vesicles
GC Content Increased at CpG Flanking Positions of Fish Genes Compared with Sea Squirt Orthologs as a Mechanism for Reducing Impact of DNA Methylation
Background: Fractional DNA methylation in sea squirts evolved to global DNA methylation in fish. The impact of global DNA methylation is reflected by more CpG depletions and/or more A/T to G/C changes at CpG flanking positions due to context-dependent mutations of methylated CpG sites. Methods and Findings: In this report, we demonstrate that the sea squirt genes have undergone more CpG to TpG/CpA substitutions than the fish orthologs using homologous fragments from orthologous genes among Ciona intestinalis, Ciona savignyi, fugufish and zebrafish. To avoid premature transcription, the TGA sites derived from CGA were largely converted to TGG in sea squirt genes. By contrast, a significant increment of GC content at CpG flanking positions was shown in fish genes. The positively selected A/T to G/C substitutions, in combination with the CpG to TpG/CpA substitutions, are the sources of the extremely low CpG observed/expected ratios in vertebrates. The nonsynonymous substitutions caused by the GC content increase have resulted in frequent amino acid replacements in the directions that were not noticed previously. Conclusion: The increased GC content at CpG flanking positions can reduce CpG loss in fish genes and attenuate the impact of DNA methylation on CpG-containing codons, probably accounting for evolution towards vertebrates. © 2008 Wang, Leung.published_or_final_versio
Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity.
Basal progenitors (BPs), including intermediate progenitors and basal radial glia, are generated from apical radial glia and are enriched in gyrencephalic species like humans, contributing to neuronal expansion. Shortly after generation, BPs delaminate towards the subventricular zone, where they further proliferate before differentiation. Gene expression alterations involved in BP delamination and function in humans are poorly understood. Here, we study the role of LGALS3BP, so far known as a cancer biomarker, which is a secreted protein enriched in human neural progenitors (NPCs). We show that individuals with LGALS3BP de novo variants exhibit altered local gyrification, sulcal depth, surface area and thickness in their cortex. Additionally, using cerebral organoids, human fetal tissues and mice, we show that LGALS3BP regulates the position of NPCs. Single-cell RNA-sequencing and proteomics reveal that LGALS3BP-mediated mechanisms involve the extracellular matrix in NPCs' anchoring and migration within the human brain. We propose that its temporal expression influences NPCs' delamination, corticogenesis and gyrification extrinsically
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