L’inconsistenza della diagnosi di cancro duttale in situ

Background: Classifying ductal carcinoma in situ (DCIS) remains problematical for interpretation of specified histological features and field selection where morphology is heterogeneous. Emphasis is placed on the role of intraductal proliferative lesions as risk factors of variable magnitude in subsequent development of invasive breast carcinoma. The present study is designed to investigate the relative contribution of diagnostic and treatment paradigms on the lack of consistency in the analysis of these lesions. Materials and methods: A cohort of in situ lesions of the breast was reviewed according to ductal intraepithelial neoplasia (DIN) classification adopted by the World Health Organization (WHO). In this retrospective study 458 patients with DIN diagnosed by core needle biopsy had undergone conservative or radical surgical treatment and SLNB in cases of DIN1C-DIN3. Results: Breast conservative surgery was the definitive treatment in 80% of cases. All the SLNs sampled showed 1.2 % (4/336) positivity of metastatic or micrometastatic nodal involvement by H&E stain while the IHC assessment for cytokeratin showed 4.5% (15/336) positivity. Conclusions: The widely recognised variation in the growth pattern of DCIS makes difficult to identify uniform indications for clinical procedure. Although the incidence of metastases in patient with initial diagnosis of DIN is considered to be low, SLNB remains an attractive option to guarantee oncological safety. In patients at high risk occult invasion does occur and without lymphatic analysis, undertreatment is possible

PROXIMITY EFFECT OF THE CONTACTS ON ELECTRON TRANSPORT IN MESOSCOPIC DEVICES

The Wigner function formalism is used for studying electron quantum transport in mesoscopic systems. In this work we show that, if the correlation of the electron wave function vanishes outside the region of interest (for example inside the contacts), then transport is affected inside the device. This property is verified analytically. Results show that, for very short devices, tunneling is actually influenced by the distance between the contacts. Modification in the electron density and conductivity have been numerically observed

Influence of contacts on the electron transport dynamics inside a mesoscopic system

In mesoscopic systems the Wigner function picture is used for studying electron quantum transport. In this work we present theoretical results on the effect of contact proximity in nanometric devices. If the correlation of the electron wavefunction vanishes inside the contact regions, the transport properties inside the device are affected. We have verified this influence analytically. The main effect is the dependence of the tunnel current through potential barriers on the distance between the contacts. Using three different simulation schemes we have observed modifications in the electron density and conductance for simple devices depending on the distance between the contacts

Theranostic in early detection of recurring prostate cancer: 64CuCl2

Aim: To evaluate the use of PET/CT with 64CuCl2 in recurring prostate cancer after surgery in patients with low PSA level (<2). Introduction: Previous works* demonstrated as 64CuCl2 is a PET probe in prostate cancer in mice with xenografts of prostate cancer.Moreover, it was described** in seven patients the role of 64CuCl2 PET/CT in staging of prostate cancer. Method: 32 Patients with low PSA level (<2) in recurring prostate post-surgical cancer were studied with 64CuCl2 PET/CT executed at 1, 3 and 24 h after injection of 200–400 MBq (medium activity 4 MBq/kg). A dosimetric analysis was done with manual VOI on various organs (Liver, Kidneys, Spleen, lumbar spine L4-L5 and Prostate as target). Coregistered imaging with CT to calculate VOIs, specific activity (Bq/ml) and SUVmax were executed. We analyzed time/activity curve with Olinda/EXM software to calculate absorbed dose. All patients had a control of PSA 10 days after PET/CT scan. Results: The PSA value was 1.1 ng/ml (0.4–1.9 ng/ml). All patients showed lesions with an uptake of 64CuCl2 with a ratio lesion/background grate than 10. The positive lesions (n=63) had a medium dimension of 5.2 mm (3–9 mm) in linfonodes (n=46), Prostatic lodge (n=13) and Bone (n=4). After 10 days all patients presented a reduction of PSA with a medium of 0.3 ng/ml (0.1–0.5). Sensibility 90%. Conclusion: PET/CT scan with 64CuCl2 is a high sensitivity method to detect early recurring prostate cancer after surgery. The possibility to see the lesions in early stage and in low dimensions (3–9 mm) opens a new possibility for theranostic application. The reduction value of PSA after 10 days could be related to the therapeutic effect of 64CuCl2. When the lesions are very small, a diagnostic dose could be also therapeutic. Further studies are necessary, but this could be a new approach in theranostic application

Mesenchymal progenitors aging highlights a mir-196 switch targeting HOXB7 as master regulator of proliferation and osteogenesis

Human aging is associated with a decrease in tissue functions combined with a decline in stem cells frequency and activity followed by a loss of regenerative capacity. The molecular mechanisms behind this senescence remain largely obscure, precluding targeted approaches to counteract aging. Focusing on mesenchymal stromal/stem cells (MSC) as known adult progenitors, we identified a specific switch in miRNA expression during aging, revealing a miR-196a upregulation which was inversely correlated with MSC proliferation through HOXB7 targeting. A forced HOXB7 expression was associated with an improved cell growth, a reduction of senescence, and an improved osteogenesis linked to a dramatic increase of autocrine basic fibroblast growth factor secretion. These findings, along with the progressive decrease of HOXB7 levels observed during skeletal aging in mice, indicate HOXB7 as a master factor driving progenitors behavior lifetime, providing a better understanding of bone senescence and leading to an optimization of MSC performance

Agricoltura conservativa - 8 anni di esperienze in Veneto

La pubblicazione raccoglie i risultati di una serie di Progetti svolti nell\u2019arco temporale 2011-2018 e in parte ancora in corso, condotti da Regione del Veneto, da Veneto Agricoltura e dall\u2019Universit\ue0 di Padova