Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Azo dyes represent the major class of dyestuffs. They are metabolised to the corresponding amines by liver enzymes and the intestinal microflora following incorporation by both experimental animals and humans. For safety evaluation of the dermal exposure of consumers to azo dyes from wearing coloured textiles, a possible cleavage of azo dyes by the skin microflora should be considered since, in contrast to many dyes, aromatic amines are easily absorbed by the skin. A method for measuring the ability of human skin flora to reduce azo dyes was established. In a standard experiment, 361011 cells of a culture of Staphylococcus aureus wereincubatedinsyntheticsweat (pH 6.8, final volume 20 mL) at 288C for 24 h with Direct Blue 14 (C.I. 23850, DB 14). The reaction products were extracted and analysed using HPLC. The reduction product o-tolidine (3,3'-dimethylbenzidine, OT) could indeed be detected showing that the strain used was able to metabolise DB 14 to the corresponding aromatic amine. In addition to OT, two further metabolites of DB 14 were detected. Using mass spectrometry they were identified as 3,3'-dimethyl-4-amino-4'-hydroxybiphenyl and 3,3'-di methyl-4-aminobiphenyl. The ability to cleave azo dyes seems to be widely distributed among human skin bacteria, as, under these in vitro conditions, bacteria isolated from healthy human skin and human skin bacteria from strain collections also exhibited azo reductase activity. Further studies are in progress in order to include additional azo dyes and coloured textiles. At the moment, the meaning of the results with regard to consumer health cannot be finally assessed

An Approach to Oobtain a PLC Program from a DEVS Model

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Analytic regularity for a singularly perturbed system of reaction-diffusion equations with multiple scales: proofs

We consider a coupled system of two singularly perturbed reaction-diffusion equations, with two small parameters $0< \epsilon \le \mu \le 1$, each multiplying the highest derivative in the equations. The presence of these parameters causes the solution(s) to have \emph{boundary layers} which overlap and interact, based on the relative size of $\epsilon$ and $$. We construct full asymptotic expansions together with error bounds that cover the complete range $0 < \epsilon \leq \mu \leq 1$. For the present case of analytic input data, we derive derivative growth estimates for the terms of the asymptotic expansion that are explicit in the perturbation parameters and the expansion order

High power heating of magnetic reconnection in merging tokamak experimentsa)

Significant ion/electron heating of magnetic reconnection up to 1.2 keV was documented in two spherical tokamakplasma merging experiment on MAST with the significantly large Reynolds number R∼10⁵. Measured 1D/2D contours of ion and electron temperatures reveal clearly energy-conversion mechanisms of magnetic reconnection: huge outflow heating of ions in the downstream and localized heating of electrons at the X-point. Ions are accelerated up to the order of poloidal Alfven speed in the reconnection outflow region and are thermalized by fast shock-like density pileups formed in the downstreams, in agreement with recent solar satellite observations and PIC simulation results. The magnetic reconnection efficiently converts the reconnecting (poloidal) magnetic energy mostly into ion thermal energy through the outflow, causing the reconnectionheating energy proportional to square of the reconnecting (poloidal) magnetic field Brec²  ∼  Bp². The guide toroidal field Bt does not affect the bulk heating of ions and electrons, probably because the reconnection/outflow speeds are determined mostly by the external driven inflow by the help of another fast reconnection mechanism: intermittent sheet ejection. The localized electron heating at the X-point increases sharply with the guide toroidal field Bt, probably because the toroidal field increases electron confinement and acceleration length along the X-line. 2D measurements of magnetic field and temperatures in the TS-3 tokamak merging experiment also reveal the detailed reconnectionheating mechanisms mentioned above. The high-power heating of tokamak merging is useful not only for laboratory study of reconnection but also for economical startup and heating of tokamakplasmas. The MAST/TS-3 tokamak merging with Bp > 0.4 T will enables us to heat the plasma to the alpha heating regime: Ti > 5 keV without using any additional heating facility.This work was supported by a Grant-in-Aid for Scientific Research (A) No 22246119 and JSPS Core-to-Core program No 22001, the JSPS Institutional Program for Young Researcher Overseas Visits and NIFS Collaboration Research Programs (NIFS11KNWS001, NIFS12KLEH024, NIFS11KUTR060). This work was funded partly by the RCUK Energy Program under Grant No. EP/I501045 and the European Communities under the contract of CCFE

Spin-Nematic Squeezed Vacuum in a Quantum Gas

Using squeezed states it is possible to surpass the standard quantum limit of measurement uncertainty by reducing the measurement uncertainty of one property at the expense of another complementary property. Squeezed states were first demonstrated in optical fields and later with ensembles of pseudo spin-1/2 atoms using non-linear atom-light interactions. Recently, collisional interactions in ultracold atomic gases have been used to generate a large degree of quadrature spin squeezing in two-component Bose condensates. For pseudo spin-1/2 systems, the complementary properties are the different components of the total spin vector , which fully characterize the state on an SU(2) Bloch sphere. Here, we measure squeezing in a spin-1 Bose condensate, an SU(3) system, which requires measurement of the rank-2 nematic or quadrupole tensor as well to fully characterize the state. Following a quench through a nematic to ferromagnetic quantum phase transition, squeezing is observed in the variance of the quadratures up to -8.3(-0.7 +0.6) dB (-10.3(-0.9 +0.7) dB corrected for detection noise) below the standard quantum limit. This spin-nematic squeezing is observed for negligible occupation of the squeezed modes and is analogous to optical two-mode vacuum squeezing. This work has potential applications to continuous variable quantum information and quantum-enhanced magnetometry

Proton Driven Plasma Wakefield Acceleration

Plasma wakefield acceleration, either laser driven or electron-bunch driven, has been demonstrated to hold great potential. However, it is not obvious how to scale these approaches to bring particles up to the TeV regime. In this paper, we discuss the possibility of proton-bunch driven plasma wakefield acceleration, and show that high energy electron beams could potentially be produced in a single accelerating stage.Comment: 13 pages, 4 figure

Effects of dental probing on occlusal surfaces - A scanning electron microscopy evaluation

The aim of this clinical-morphological study was to investigate the effects of dental probing on occlusal surfaces by scanning electron microscopy (SEM). Twenty sound occlusal surfaces of third molars and 20 teeth with initial carious lesions of 17- to 26-year-old patients (n = 18) were involved. Ten molars of each group were probed with a sharp dental probe (No. 23) before extraction; the other molars served as negative controls. After extraction of the teeth, the crowns were separated and prepared for the SEM study. Probing-related surface defects, enlargements and break-offs of occlusal pits and fissures were observed on all occlusal surfaces with initial carious lesions and on 2 sound surfaces, respectively. No traumatic defects whatsoever were visible on unprobed occlusal surfaces. This investigation confirms findings of light-microscopic studies that using a sharp dental probe for occlusal caries detection causes enamel defects. Therefore, dental probing should be considered as an inappropriate procedure and should be replaced by a meticulous visual inspection. Critical views of tactile caries detection methods with a sharp dental probe as a diagnostic tool seem to be inevitable in undergraduate and postgraduate dental education programmes. Copyright (c) 2007 S. Karger AG, Basel

Coupled Systems of Differential-Algebraic and Kinetic Equations with Application to the Mathematical Modelling of Muscle Tissue

We consider a coupled system composed of a linear differential-algebraic equation (DAE) and a linear large-scale system of ordinary differential equations where the latter stands for the dynamics of numerous identical particles. Replacing the discrete particles by a kinetic equation for a particle density, we obtain in the mean-field limit the new class of partially kinetic systems. We investigate the influence of constraints on the kinetic theory of those systems and present necessary adjustments. We adapt the mean-field limit to the DAE model and show that index reduction and the mean-field limit commute. As a main result, we prove Dobrushin's stability estimate for linear systems. The estimate implies convergence of the mean-field limit and provides a rigorous link between the particle dynamics and their kinetic description. Our research is inspired by mathematical models for muscle tissue where the macroscopic behaviour is governed by the equations of continuum mechanics, often discretised by the finite element method, and the microscopic muscle contraction process is described by Huxley's sliding filament theory. The latter represents a kinetic equation that characterises the state of the actin-myosin bindings in the muscle filaments. Linear partially kinetic systems are a simplified version of such models, with focus on the constraints.Comment: 32 pages, 18 figure